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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Plant Biology 2022Quote: ... 1x phosphatase inhibitor cocktail 2 and 3 from Sigma) to release membrane proteins ...
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bioRxiv - Neuroscience 2022Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma)) and incubated on ice for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... EHMT1/2 inhibitor (Sigma-Aldrich, 3 mg/mL), SB747651A ...
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bioRxiv - Cell Biology 2022Quote: ... and Phosphatase inhibitors cocktail 2 and 3 (Sigma). Lysed cells were harvested by scraping ...
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bioRxiv - Cell Biology 2024Quote: ... and phosphatase inhibitors cocktail 2 and 3 (Sigma) one ice for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors (cocktail 2 and 3, Sigma). Samples were then sonicate for 1 minute and a BCA assay was used to determine the protein concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors cocktail 2 and 3 (Sigma). The cells were lysed for 30 to 45 minutes at 4°C followed by centrifugation at 14000 RPM for 40 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Developmental Biology 2024Quote: The SHMT2 gene was cloned from cDNA of HeLa cells using primers 5’-GCCCATATGGCCATTCGGGCTCAGCAC-3’ (forward) and 5’-GCCCTCGAGATGCTCATCAAAACCAGGCA-3’ (reverse) and subcloned into the pET41a vector (Novagen, WI, USA) at restriction enzyme sites of NdeI and XhoI ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Immunology 2021Quote: Three- to 4-week-old mice were injected intraperitoneally twice with 0.25 mg 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 2 hours apart ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Microbiology 2024Quote: ... in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst or 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI)) or ExtrAvidinCy3 (Sigma) solution (1:100 in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst) ...
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bioRxiv - Biochemistry 2022Quote: ... by plating them on NB agar medium (2% glucose, Ade, Ura) supplemented with different concentrations (5-50mM) of 3-aminotriazole (3AT; Sigma-Aldrich) histidine inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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bioRxiv - Microbiology 2020Quote: ... were passaged 2-3 times weekly and grown at 37° C with 5% CO2 in DMEM containing 10 mM L-glutamine (Sigma, M8537), supplemented with 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Microbiology 2021Quote: ... or the same isosmotic solution with 100 µM of the general anion channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, Sigma-Aldrich), which inhibits malarial new permeation pathways (59) ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... Flash-frozen plant samples (0.2 g) were ground and extracted using 2 mL of 3 % 5-sulfosalicylic acid (Sigma, ON, Canada). The extract was centrifuged for 10 min at 8050 x g at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Dissected tumors were washed in PBS and homogenized at room temperature in urea lysis buffer (8 M urea, 40 mM Tris pH 7.6, 5% SDS supplemented with phosphatase inhibitor cocktails 2, 3 (Sigma P5726, P0044)) ...
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bioRxiv - Bioengineering 2022Quote: ... HA-TBA was dissolved in anhydrous DMSO (2 wt%) with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (BC-1, BCBL-1) or 20% (BC-2, BC-3, BC-5, BJAB) Serum Plus-II (Sigma-Aldrich, catalog number 14009C ...
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bioRxiv - Immunology 2021Quote: ... and MEM supplemented with 1 μg/ml N-tosyl-L-phenylalanine chloromethyl ketone (TPCK)-treated trypsin (Sigma) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 5×10−5 M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), recombinant murine IL-3 (0.2 ng/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μM 5-fluoro-2’-deoxyuridine (Sigma Aldrich 200-072-5), penicillin ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM 4-thiouracil (4tU) (Sigma, 440736-1G) was added to each condition during the final 6 minutes of auxin treatment ...
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bioRxiv - Biochemistry 2024Quote: ... 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in DMSO was used ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 ng/mL IL-4 (Sigma, I1020). The cells were cultured for 72 hours at 37°C in a 5% CO2 incubator.
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bioRxiv - Neuroscience 2021Quote: ... 5 μM carbonyl cyanide 3-chlorophenylhydrazone (Sigma Cat # C2759) and 5 μM valinomycin (Sigma Cat # V0627) ...
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bioRxiv - Genomics 2021Quote: ... a 3-day puromycin (5 μg/mL, Sigma, P8833) selection was performed 48 hours after the spin-inoculation ...
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bioRxiv - Biophysics 2020Quote: ... mglBΔCT-R (5’-CGTAAAGCTTTTACACCAGGCTCTCGAAGATCTTCGTGAGCTC-3’ synthesized by Sigma-Aldrich, India ...
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bioRxiv - Neuroscience 2021Quote: ... or non-targeting siLUC (5’-UAAGGCUAUGAAGAGAUAC-3’, Sigma-Aldrich) were mixed with 54 μl of RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... we used the siRNA duplex 5′-UUCUCCGAACGUGUCACGUdTdT-3′ (Sigma). The cells were further processed according to the experimental design and depletion was assessed by immunofluorescence ...
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bioRxiv - Genetics 2024Quote: ... moxidectin (3 nM) (Millipore Sigma, Catalog # 113507-06-5), ricobendazole (25 μM ...
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bioRxiv - Cell Biology 2022Quote: ... 50 nM Rab14 siRNA (5′-CAACUACUCUUACAUCUUU-3′, Sigma-Aldrich) for 72 h using Lipofectamine 2000 ...
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bioRxiv - Genetics 2023Quote: ... scrambled (NT) shRNA (5’-GCGATAGCGCTAATAATTT-3’ SHC202; Sigma-Aldrich) or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence ...
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bioRxiv - Microbiology 2024Quote: ... a 5% solution of 3-Aminopropyltriethoxysilane (APTES, Sigma-Aldrich) in tetrahydrofuran (THF ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Biochemistry 2023Quote: ... and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich). Eluate was concentrated at 4°C (Corning Spin-X concentrators) ...
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bioRxiv - Genomics 2024Quote: ... Samples were stained with 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542) for 5-10 minutes at room temperature manually and rinsed three times with 1xTBS with 0.1% (v/v ...
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bioRxiv - Genomics 2024Quote: ... formamide in 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542). Readout probes ...