Labshake search
Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
-
bioRxiv - Cell Biology 2022Quote: ... activated eggs were cultured for 3 additional hours in KSOM + amino acids media (Sigma Aldrich, Cat# MR-106-D). For parthenogenetic activation of eggs ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with 50 µL of a 2 mg/mL solution of (3-(4,5-dimethyl-thiazole-2-yl)-2,5-biphenyl tetrazolium (MTT, Sigma-Aldrich) in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO ...
-
bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
-
bioRxiv - Microbiology 2022Quote: Cells were then pelleted at 7000 × g for 3 min and resulting supernatants filtered through a 0.2 μm PVDF filter (Millipore). The no bacteria “fresh” 5-FU controls were also filtered in this way to account for any potential loss of 5-FU by filtration ...
-
bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
-
bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
-
bioRxiv - Biochemistry 2022Quote: ... or 300 mM (octamer-mix + APLFAD) ammonium acetate at pH 7.5 using 3 kDa MWCO Amicon Ultra Centrifugal Filter Units (Merck Millipore). After buffer exchange the volume of each sample was ∼40 µL ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The silanization solution was freshly prepared by mixing MilliQ water with 0.4% (v/v) 3-(Trimethoxysilyl)propylmethacrylat (TMSPMA, Sigma) and 0.4% (v/v ...
-
bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
-
bioRxiv - Molecular Biology 2023Quote: ... mice were intraperitoneally injected with tamoxifen (0.08mg/g body weight per day for 3 days; Sigma-Aldrich, T5648-1G). For chemical-treated animals’ assay ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were harvested and lysed by 3 cycles of thawing and freezing followed by treatment with benzoase nuclease (Millipore). After pelleting down cell debris (16.9 g/rcf ...
-
bioRxiv - Neuroscience 2023Quote: ... and then received 0.1mL of either 1.5-3% Fluorescein isothiocyanate–dextran (FITC) or Texas Red dextran (70kDa, Sigma-Aldrich #46945 and Thermofisher D1830 ...
-
bioRxiv - Neuroscience 2022Quote: ... Fish were then anesthetized by applying 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521) to the fish mounted in agarose ...
-
bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 0.5 mL centrifugal filter units (3-kDa molecular weight cutoff, Millipore), using centrifugation at 14,000 g ...
-
bioRxiv - Neuroscience 2023Quote: ... From day 3 to day 15 1µM retinoic acid (SigmaAldrich, R2625) and 1µM Sonic Hedgehog Signaling Agonist (Millipore, 566660) were added ...
-
bioRxiv - Plant Biology 2023Quote: ... above the tips with the help of forceps and placed into a 35-mm-diameter dish containing a 70 µm strainer and 3 ml enzyme solution (1.25% [w/v] Cellulase [“ONOZUKA” R10, Yakult], 0.1% [w/v] Pectolyase [P-3026, Sigma-Aldrich] ...
-
bioRxiv - Systems Biology 2023Quote: Buffy Coat was diluted with PBS at a 1:3 dilution and underlayed with Histopaque®-1077 (Sigma-Aldrich), centrifuged at 350g for 30 minutes at 24°C with no brake ...
-
bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report70 ...
-
bioRxiv - Neuroscience 2023Quote: ... 100 μg/mL streptomycin and 1 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic sodium monophosphate salt (dbcAMP) (Sigma®). Cells were maintained with the differentiation medium at 37 °C in 5% CO2 for 3 days.
-
bioRxiv - Physiology 2022Quote: ... cells were treated with the medium supplemented with 0.5 mM 3’-Isobutyl-1-methylxanthine (IBMX) (Sigma, Cat. I7018-1G), 1 µM dexamethasone (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
-
bioRxiv - Cell Biology 2022Quote: ... 6- to 8-week-old mice of the indicated genotypes were intraperitoneally injected with 2.5 ml 3% thioglycollate (T9032, Sigma). On day 4 after the thioglycollate injection ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL aliquots were taken from the main culture for chemical fixation with 3% paraformaldehyde (PFA, Sigma-Aldrich, #F8775) at 20 min at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
-
bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hrs for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich).
-
bioRxiv - Plant Biology 2022Quote: Analytical reference standards were used for the following analytes: indole-3-acetic acid (IAA; Sigma-Aldrich St. Louis, MO), N-(3-indolylacetyl)-DL-aspartic acid (IAA-Asp ...
-
bioRxiv - Developmental Biology 2022Quote: ... BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat. M8226], 0.1% IGEPAL [v/v; Sigma, I8896] ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 mM HEPES-KOH, pH 7.2 [Fisher, Cat. BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat ...
-
bioRxiv - Bioengineering 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% dimethylsulfoxide (DMSO ...
-
bioRxiv - Plant Biology 2024Quote: ... After immunolabeling the coverslips were incubated in Acryloyl-X-SE 0.1 mg/mL in PBS (ThermoFischer) overnight at RT and then in the monomer solution (11.7% NaCl, 3 % acrylamide (Sigma), 0.15% N,N′-methylenebisacrylamide (Sigma) ...
-
bioRxiv - Neuroscience 2024Quote: ... microglia were treated with the respective concentrations of selected drugs (see Table 3) or DMSO (Sigma-Aldrich, Cat #:472301) as control and incubated for 6hrs and 24hrs before harvest for RNA extraction ...
-
bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
-
bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1402-9500) containing 3 μL of SMART-seq3 lysis buffer (0.5 μL PEG 8000 (40% solution, Sigma Aldrich, P1458), 0.03 μL Triton X-100 (10% solution) ...
-
bioRxiv - Neuroscience 2023Quote: ... Germany) containing the steroid hormone RU486 (200 μg/ml, CAS No.: 84371-65-3, Sigma-Aldrich, St. Louis, MO) for two days.
-
bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
-
bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: Articular cartilage explants from the non-OA donors were treated with 3 μM of Wnt agonist CHIR99021 (Sigma-Aldrich) or with 1 μL/mL of its vehicle (V ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with blocking buffer (PBS containing 0.1% Triton X-100 and 3% fatty-acid-and globulin-free BSA [A7030, Lot No. SLCH7959, SIGMA]) for 1 h at room temperature (approximately 26-28°C ...
-
bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
-
bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
-
bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
-
bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
-
bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
-
bioRxiv - Molecular Biology 2023Quote: ... pregnant females were ovariectomized on E2.5 and afterwards injected with 3 mg medroxyprogesterone 17-acetate subcutaneously (Sigma, M-1629) on E3.5 and E5.5 ...
-
bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...