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Citations for Millipore Sigma :
8401 - 8450 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... were seeded in DMEM supplemented with 10 % FBS in 96 well plates incubated with increased concentrations of compound 2 and treated with 3-NP at 10 mM (#N5636, Sigma). Then ...
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bioRxiv - Cell Biology 2020Quote: ... cells were replica-plated onto pABA− (2% glucose, w/v) plates with 0 µM or 25 µM linolenic acid (C18:3, Sigma). Colonies that grew on 25 µM linolenic acid were picked into YEPD overnight cultures and struck on YEPD plates ...
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bioRxiv - Biochemistry 2022Quote: ... cells were washed in 60% isopropanol and stained in an ORO solution (2:3 v/v H2O: isopropanol, containing 0.5% ORO, Sigma O0625) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The surface was regenerated with two pulses (30 s at 100 µL/min) of Isopropanol:50 mM NaOH (2:3) solution followed by a 20 mM CHAPS (Sigma) or 40mM Octyl-beta-Glucoside (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 3 days post-fertilization (dpf) zebrafish larvae were anaesthetized for 2 - 3 minutes in embryo medium E3 containing 0.2 mg/ml Tricaine (Sigma-Aldrich). Thereafter ...
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bioRxiv - Neuroscience 2021Quote: ... next permeabilized for 20 minutes in PBST (PBS with 0.3% Triton X-100; BioShop) and blocked for 2 h in 3% BSA (Sigma-Aldrich) in PBST at RT ...
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bioRxiv - Cell Biology 2019Quote: ... injected embryos were dechorionated and embedded in 2-mm or 3-mm glass capillaries (Hilgenberg GmbH) filled with 0.9% low melting temperature agarose (Type VII, Sigma Aldrich) prepared in zebrafish system water ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Microbiology 2020Quote: Caco-2 cells were seeded at 2 × 105 cells/well onto polycarbonate Millicell culture-plate inserts (12-mm diameter, 3-μm pore size; Millipore) and cultured for 5 days ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed 3 times for 2 min in 0.01 M PBS containing 0.2% Triton X-100 (Tx) (PBS-Tx; Sigma-Aldrich, T8787) and then were pre-treated with 3% H2O2 in PBS-Tx for 10 min ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... sodium dodecyl phosphate (SDS) 0.1%, 25mM Tris) supplemented with protease and phosphates inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3) (Sigma-Aldrich) and containing 4% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2021Quote: Primary polyps were incubated in freshly filtered (0.2 μm) seawater with 3 μM calcein blue (Sigma–Aldrich 54375-47-2) for 4-5 hours ...
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bioRxiv - Immunology 2020Quote: ... 8-20 weeks after reconstitution chimeric mice were injected intraperitoneally for 3 d with 2 mg/day of tamoxifen (Sigma) re-suspended at 20 mg/ml in corn oil (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Slices were transferred to Millicell-CM membrane inserts (0.4 μm pore size, 30 mm diameter, 2-3 slices per insert; Millipore Corp) in six well tissue culture treated plates containing MEM with 25% horse serum and 6.5 mg/mL of glucose ...
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bioRxiv - Immunology 2021Quote: ... were pelleted at 3.000g for 3 minutes and induced by resuspension in YNB-CAA + 2% D-(+)-Galactose (Sigma G0625-500G) + phosphate buffer + P/S induction medium at a final OD600 of 0.5 ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were kept at dim light (2-3 μE) for 16 h and plated on fresh TAP plates containing 10 μg/mL Paramomycin (Sigma). Plates were incubated for 10 d at 30-50 μE constant light ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 500 μL 2% Alizarin Red solution (Sigma-Aldrich, 05600) diluted in UPW for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 0.1 % Tween-20 and 2 mM EDTA before incubating in a blocking solution (3 % acetylated bovine serum albumin (Sigma, MO), 0.1 % Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... Liquid cultures were grown in a mixture of 2:3 ratio of Todd-Hewitt media with 0.5% yeast extract (Sigma-Aldrich), and Brain-Heart Infusion media (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... media was exchanged for 2 mL of culture media supplemented with 500 µM 3-indoleacetic acid (IAA, Millipore Sigma, I2886). Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... cells were synchronised in early S phase by two consecutive rounds of treatment with 2 mM thymidine and 3 ug/ml (Sigma) aphidicolin ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were incubated for 30 minutes with 3 µmol/L 4,5-diaminofluorescein diacetate (DAF-2 DA; Sigma-Aldrich, D225). After that ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were housed singly for 2–3 days after the procedure and were subsequently head-fixed to a custom mount with eicosane (Sigma) and imaged on a Movable Objective Microscope with resonant scanners (MOM ...
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bioRxiv - Genetics 2023Quote: ... animals of similar age were depleted of AID::GFP expression by culturing for 2 h on NGM plates containing 100 µM auxin (3-indoleacetic acid; Sigma). Directly after depletion ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were inoculated in YEP containing 3% lactic acid and treated with a final concentration of 2% galactose (Sigma-Aldrich). For the inducible degradation of the proteins ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mm3 tissue pieces from 2-3 brains were pooled and enzymatically digested in a dissociation medium containing 0.16 mg/mL cysteine (Sigma C9768), 7 U/mL Papain (Sigma P3125) ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Immunology 2023Quote: ... The activity of trypsin was inhibited by dropping the pH to 2-3 by the addition of 10% trifluoroacetic acid (TFA, Sigma). The samples were loaded on Evosep tips to separate the digested peptides using nanoflow reversed-phase chromatography with an Evosep One liquid chromatography (LC ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis Ws-2 CCR2::LUC WT seeds were surface sterilized and plated on 1x MS medium containing 3% w/v sucrose (Sigma), 1.5% w/v phytoagar (Duchefa) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Microbiology 2021Quote: ... The blocks were infiltrated with a 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich, St. Louis, USA) for 1 h at room temperature and 1.5 h incubation with 2% OsO4 ...
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bioRxiv - Neuroscience 2019Quote: ... All cells used in this study were exposed to 1 µM 4-hydroxytamoxifen (Sigma, Cat#: H6278) for 24 hours prior to studies of Ucp2 function.
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bioRxiv - Neuroscience 2019Quote: ... All cells used in this study were exposed to 1 µM 4-hydroxytamoxifen (Sigma, Cat#: H6278) for 24 hours prior to studies of Ucp2 function.
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bioRxiv - Developmental Biology 2019Quote: ... Sections were incubated overnight at 4°C with monoclonal mouse anti-NG2 (Sigma, C8035, 1:100), mouse anti-SOX2 (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... for 1 hour at 4°C prior to permeabilization with 0.1% triton X-100 (Sigma-Aldrich) and incubation in blocking buffer containing 10% goat serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: Amoxicillin trihydrate: potassium clavulanate (4:1) (AMC) was diluted in molecular grade water (both Sigma, UK) to a stock concentration of 1 mg/ml and filter sterilised through a 0.22 μM polyethersulfone filter unit (Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... was added to the medium at 4 mM together with 1% Igepal CA-630 (Sigma-Aldrich). Cells were stained with 0.5 μg/ml BODIPY 493/503 (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Guts were stained over night at 4°C with Phalloidin-Atto-550 (1:3000; Sigma-Aldrich), washed three times with PBS and embedded in Vectashield (Vector Laboratories).
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated overnight at 4 °C with the polyclonal antibody ATB0,+ (1:100, Sigma-Aldrich), then washed with PBS and further incubated for 45 min with Alexa Fluor 488 antibody (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of matrix solution composed of saturated α-cyano-4-hydroxycinnamic acid (Sigma, Lyon, France), 50% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Neuroscience 2022Quote: ... on 25 × 75 × 1 mm glass slides using Mowoil 4-88 mounting medium (Sigma-Aldrich: 81381). Mounting medium was set at 4°C and slides were imaged within 1 hour.
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with rabbit anti-tyrosine hydroxylase (1:1000 Millipore, USA) in PBS-T and 2% NDS solution ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 1 hour at room temperature with 4% BSA (Millipore Sigma, #82-067) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4 °C in guinea pig anti-VGLUT1 (1:1000, Millipore AB5905) or guinea pig anti-VGLUT2 (1:8000 ...
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bioRxiv - Cancer Biology 2021Quote: ... SHP2i was diluted in 50 mM Sodium Citrate Buffer pH = 4 with 1% Hydroxyethylcellulose (Sigma-Aldrich), 0.25% Tween (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were subsequently incubated overnight at 4°C with primary antibodies (S100β 1/200, Sigma S2532 ...
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bioRxiv - Neuroscience 2021Quote: Colonies at selected phases (Tables 1-4) were anaesthetized with MS222 (tricaine methanesulfonate, Sigma, A5040-25) and fixed in 4% PFA in MOPS (0,1 M MOPS ...