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Citations for Millipore Sigma :
8601 - 8650 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... resuspended at 2 × 107 / ml and incubated with 2 µg / ml anti BrdU antibodies (Sigma B8434) for 2 hrs at RT ...
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bioRxiv - Bioengineering 2019Quote: ... HDI crosslinker (#52649) and 2-(trifluoromethyl)phenyl isocyanate (2-TPI) (#159379) were purchased from Sigma Aldrich. N,N-dimethylformamide (DMF ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein sample (400 μg) was reduced by adding 2 μl of Tris(2-carboxyethyl)phosphine (Sigma) and incubating samples at 60 °C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... The retinas were dissected and embedded in 2% low melting agar (2-hydroxymethyl agarose, Sigma Aldrich), mounted on a vibratome (DSK Microslicer ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 2 buffer with 25% w/v (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD, Sigma-Aldrich). Treatments were aliquoted into single and combined daily doses and frozen at -80 ºC ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated for 2 hours at room temperature with 2% normal donkey serum (Sigma, G6767) in PBS overnight at 4 °C with c-Fos primary antibodies (226003 ...
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bioRxiv - Microbiology 2024Quote: ... 2-hydroxyibuprofen (2-OH-IBU) and carboxyibuprofen (CBX-IBU) were obtained from Sigma-Aldrich (Steinheim, Germany). All chemicals and solvents used were of the highest purity available.
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was applied on an already equilibrated 2-(2-pyridyl)ethyl columns (Sigma 54127-U) with 1 ml of 50% MeOH with 2% acetic acid ...
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bioRxiv - Plant Biology 2023Quote: ... 2% ß-mercaptoethanol and 2 tablets/10ml of complete EDTA free protease inhibitor (Sigma Aldrich, USA), 100μM E-64 cysteine protease inhibitor (Bera et al. ...
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The RIFF: an automated environment for studying the neural basis of auditory-guided complex behaviorbioRxiv - Neuroscience 2021Quote: ... and 1:1 mixture of 4% sucrose and 0.1 % saccharine solutions (sucrose and saccharin, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Cell Biology 2019Quote: ... for 30 minutes and incubated overnight at 4°C with anti-TAT1 (1:250) and/or anti-GFP (1:500, Sigma) antibodies ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Frozen brain tissue was ground with a motorized pestle for 30 s in 100 µl of chilled (4:1) analytical grade methanol:ddH2O with 1 mM norluecine (Sigma Aldrich) standard ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with guinea pig anti-vGlut2 antiserum (1:12,000, cat# AB2251-1, Millipore Sigma) in PBS-T containing 2% normal donkey serum ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636 ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... Universal matrix composition: mixture of 1:1 of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (2,5-DHB) (Sigma-Aldrich solubilized in 78:22 MeCN in H2O + 0.1% TFA.
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bioRxiv - Neuroscience 2020Quote: ... for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I; guinea pig anti-vGlut1, 1:1000, Millipore AB5905 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Primary antibodies were incubated overnight at 4°C and included Col2 (1/100, Thermo) and Sox9 (1/1000, EMD Millipore). Negative controls included a mouse IgG (2µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were cultured in 4 mL CgXII medium containing 13C-1-glucose or 13C-1-fructose (Sigma-Aldrich, Taufkirchen, Germany) as sole carbon sources ...
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bioRxiv - Neuroscience 2019Quote: ... afterwards with secondary antibody mix for 1 day overnight at 4°C and finally with DAPI (1:10000, Sigma-Aldrich) for 8 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Microbiology 2021Quote: ... tissues were incubated until dissolved at 37°C in 495 μL extraction buffer [(4 M urea, 0.2 M NaCl, 1 mM trisodium citrate, 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Phage 80α stock was diluted in phage buffer (50 mM Tris-HCl pH 7.8, 1 mM MgSO4, 4 mM CaCl2 and 1 g/L gelatin; Sigma–Aldrich), and added to the master mix to reach the desired starting concentration in plaque forming units per mL (pfu/mL) ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed 4 times with PBS and resuspended in PBS containing 1% BSA and DAPI (1 μg/mL; Sigma) as a nuclear stain ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Microbiology 2022Quote: ... MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], malt extract 10 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2023Quote: ... the reaction between peptide conjugate and crosslinkers (PEG-4VS and PEG-4 ACLT) was performed in stoichiometric ratio 1:1 in30mM HEPES buffer at pH 8 (Sigma) diluted in 1x HBSS (Gibco) ...
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bioRxiv - Physiology 2023Quote: ... sections were exposed overnight at 4°C to antibodies detecting CARNS1 (rabbit polyclonal, 1:100 in PBS with 1% BSA, HPA038569, Sigma). The next day ...
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bioRxiv - Immunology 2023Quote: Mouse lungs were diced into small pieces using razor blades and incubated for 1 h in digestion medium (RPMI-1640 with 1 mg/mL collagenase type 4, Sigma) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... washed with distilled water and fixed on a shaker for 20 minutes in a 1:1 mix of 4% Paraformaldehyde (Proscitech) in 1xPBS and Heptane (Sigma). Embryos were then washed 3 times with absolute methanol and 3 times with PBS/T and blocked for 1 hour in 0.1% PBS/T containing 10% normal goat serum (NGS) ...
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bioRxiv - Cancer Biology 2021Quote: ... N-N-Dimethyl formamide and pLKO.1 vector was purchased from Sigma-Aldrich (St.Louis, MO, USA). BFF-122 was purchased from Axon Medchem (Groningen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM ethylene glycol-bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic acid (EGTA; Sigma, E3889), 1 mM MgSO4 (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... Detergent N,N-dimethyldodecylamine N-oxide (LDAO) was from Sigma. Columns Biosep 4000 GFC/SEC were purchased from Phenomenex ...
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bioRxiv - Cancer Biology 2021Quote: ... For AR and FOXA1 ChIP organoids were double fixed (Singh et al., 2019) with 2 mM Di(N-succinimidyl) glutarate (Millipore Signal, Cat #80424-5MG-F) and then 1% formaldehyde ...
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bioRxiv - Cell Biology 2020Quote: ... 3-Isobutyl-1-methylxanthine (IBMX, ≥ 99%), N-acetyl-L-cysteine (NAC, ≥ 99%) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥ 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pregnant mare serum gonadotropin (PMSG ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
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bioRxiv - Neuroscience 2022Quote: ... The aqueous phase was transferred to a tube containing 500 μl of isopropanol (Fisher, A416P-4) and 5 μg of glycogen (Sigma, G1767-1VL). Then samples were pelleted at 18,213 x g for 15 min ...
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bioRxiv - Immunology 2022Quote: ... and was further purified by centrifugation at 10,000 xg for 5 minutes at 4°C through an Amicon Ultra 0.5 ml 100 kDa cutoff spin filter (Millipore, Billerica, MA, USA). The NEU3 was analyzed for size and purity by PAGE on ...
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bioRxiv - Bioengineering 2021Quote: ... tissue was washed 2X in methanol 100% for 4hrs and bleached overnight at 4°C in 5% hydrogen peroxide (Sigma-Aldrich 216763) in methanol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml; Sigma-Aldrich, www.sigmaaldrich.com). We measured mass spectra using an Ultraflex III MALDI-TOF (Bruker Daltonics ...
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bioRxiv - Pathology 2019Quote: ... The membranes were then washed in PBS-T and incubated overnight at 4°C in PBS-T containing 5% milk powder and primary antibody: monoclonal anti-GFAP (Sigma-Aldrich, G3893) in dilution 1:400 or monoclonal anti-actin (MP Biomedicals ...
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bioRxiv - Cell Biology 2020Quote: ... The non-adherent cardiomyocytes were seeded in complete Cardiomyocyte plating medium supplemented with 10% fetal calf serum and antibiotics at a density of 4-5 × 105 cells/well on Poly-L-Lysine (Sigma P-7890) and Gelatin (Sigma G9891)-coated coverslips in 6-well plates ...