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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and cervical spinal cord tissues (C3-C6) were isolated (n=5-8/treatment; 1 male and 1 female per litter) and sonicated in Tri-Reagent (Sigma, St. Louis, MO, USA) and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Biophysics 2023Quote: ... 25 µM camptothecin for 1h (Sigma-Aldrich C9911), 20 µM etoposide for 1 hour (Sigma-Aldrich E1383) ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1:100 dilution of GlutaMax and penicillin/streptomycin and cells were treated (n = 3 per group) with compounds and tBHQ (Sigma 11294-1) for 24 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
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bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
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bioRxiv - Zoology 2019Quote: ... or cortisol treatments (8 g peanut butter, 2 g wheat germ with 6, 8, or 12 mg of cortisol [H4001, Sigma Aldrich, USA]). Dosages of 0 ...
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bioRxiv - Immunology 2019Quote: ... CHO cells pre-incubated with trivalent Strep-Tactin-SpyCatcher or buffer alone were incubated with 2 µM ATTO 647 biotin (ATTO Technology # AD 647-71) pre-mixed with 6 µM biotin (Sigma-Aldrich # B4501) for 30 minutes at 25 °C ...
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G; 2,46 g/L NaHCO3, Sigma Aldrich, S5761-500G ...
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bioRxiv - Developmental Biology 2022Quote: ... T8787)/PBS at 37C for 2 days and subsequently immersed in a blocking solution of 10% DMSO + 6% donkey serum (EMD Millipore: S30) + 0.2% Triton X-100/PBS at 37C for 3 days ...
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bioRxiv - Cell Biology 2024Quote: Uninduced and induced samples were treated with either 0.2 μM or 2 μM Latrunculin A in DMSO (Sigma, CAS 76343-93-6) up to 4 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Plates were then blocked for 6 h with PBST (PBS with 0.01% Tween-20) containing 2% BSA (Sigma-Aldrich; cat. no. 10735094001) at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Lineage-labeling was induced at indicated time points using fresh or pre-heated (65°C for 10 min) 4-Hydroxytamoxifen (4-OHT) (Sigma H7904) stock solutions in DMSO at a final concentration of 10 μM in E3 embryo medium (Felker et al. ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of Col1a2xLPCherry-Tg was induced by keeping animals in separated tanks with water containing (Z)-4-hydroxytamoxifen (4-OHT, Sigma T5648) 2 µM overnight ...
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bioRxiv - Biochemistry 2019Quote: ... Freshly purified CsgA was filtered using a 30 kDa cut-off column at 4°C (Amicon® Ultra-4, Sigma-Aldrich) to remove insoluble protein aggregates and seeds ...
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bioRxiv - Genetics 2020Quote: ... cells were fixed at day 4 of differentiation with 4% paraformaldehyde for 20 minutes at 4°C followed by permeabilization with 0.1% triton™X-100 (Sigma Aldrich) for 30 minutes at room temperature and blocking in 1xPBS with 1% fetal bovine serum (Gibco) ...
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bioRxiv - Plant Biology 2021Quote: ... roots of Arabidopsis thaliana Col-0 and sr45-1 mutant seedlings (or 9-week-old plants) were vacuum infiltrated with HCl (4%, v/v) and K-Ferrocyanide (II) (4%, w/v, Sigma-Aldrich) (1/1 ...
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 56 d at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×106 cells were fixed overnight in a solution containing 4% formaldehyde and 4% acrylamide in PBS and adhered to a poly-L-lysine (Sigma, P4707) coated coverslip ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Cancer Biology 2021Quote: ... were used to infect subconfluent MCF7 KRT19 KO cells in three sequential 4 h incubations in the presence of 4 μg/ml polybrene (Sigma-Aldrich). Transductants were selected in hygromycin (100 μg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... With the fluorescent GCase substrates blue fluorogenic substrate 4-methylumbelliferyl-β-D-glucopyranoside (4-MU) (Sigma-Aldrich; as described in 11,12), PBMCs were washed 2x with PBS and lysed in 60μl of activity assay buffer (50mM Citric acid ...
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bioRxiv - Neuroscience 2022Quote: ... where they were lightly anesthetized with isoflurane (to reduce stress from injection) and intraperitoneally injected with 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) at a dose of 50 mg/kg ...
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bioRxiv - Immunology 2022Quote: Itaconate (ITA) and its derivatives 4-octyl itaconate (4-OI) and Dimethyl itaconate (DMI) were purchased from Sigma-Aldrich (Deisenhofen, Germany) (ITA ...
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bioRxiv - Neuroscience 2019Quote: ... ubi:Switch double transgenic fish were immersed either for ten minutes in fish water containing 0.5 μM 4-hydroxytamoxifen (4-OHT - Sigma-Aldrich, T176) for clonal recombination of the reporter construct or 7 hours per day ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the HTER and HSER cell lines by chronic stimulation with 4 ng/ml 4-hydroxytamoxifen (4OHT; Sigma Aldrich) for 14 days9 ...
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bioRxiv - Neuroscience 2019Quote: ... with the following modifications: 4-day-old female adult brains were dissected in PBS and then fixed in 4% (wt/vol) paraformaldehyde (Sigma-Aldrich) in PBS on ice for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... TrkCCreERT2::Rosa26ChR2-YFP mice were treated with a single intrathecal (i.t.) injection of 45 ng of 4-hydroxytamoxifen (4-OH Tamoxifen, Sigma Aldrich, H7904). Experiments were performed at least one week after the treatment.
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bioRxiv - Microbiology 2020Quote: Phosphatase and lipase activity were measured by following the hydrolysis of fluorescent substrates 4-Methylumbelliferyl phosphate and 4-Methylumbelliferyl oleate (both from Sigma-Aldrich) following standard methods (Hoppe ...
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bioRxiv - Molecular Biology 2020Quote: ... The fraction was incubated for 4 hours at 4°C with 250 μl of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... obtained from the NA activity assay of the PV to a standard curve of 4-Methylumbelliferone sodium salt 4-MU(SS) (Sigma M1508). NA activity is based on the production of 4-MU over time (60 minutes at 37 °C for the standard assay) ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was incubated for 4 hrs at 4 °C with 250 µL of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... onto 4-inch silicon (Si) wafers previously coated with a sacrificial layer in poly(4-styrene-sulfonic acid) (PSS; 561223, Sigma Aldrich). Thiol-ene photopolymerization was performed under UV light (365 nm ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Evolutionary Biology 2022Quote: ... erythrogramma embryos were fixed for in-situ hybridization (ISH) for ∼16 hrs overnight at 4 °C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894 ...
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bioRxiv - Immunology 2023Quote: ... The FLAG-tagged WT sCTLA-4 or Y139A sCTLA-4 in the culture supernatant was purified with the anti-FLAG M2 affinity gel (Sigma-Aldrich), washed with wash buffer (Phosphate buffered saline ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and pelleted by centrifugation for 4 minutes at 800 x g and washed twice in vPBS before being resuspended in fixation solution consisting of 4% formaldehyde and 4% acrylamide and transferred to a 12 mm round coverslip (Sigma, P4707) coated in poly-L-lysine and placed in a 24 well plate ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
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bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
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bioRxiv - Bioengineering 2023Quote: ... or 5 µg/mL NETs for 4 hr prior to fixation with 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA) in phosphate-buffered saline (PBS, Sigma-Aldrich) for 15 min at room temperature and staining with 0.005% Crystal Violet in PBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
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bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
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bioRxiv - Bioengineering 2024Quote: ... the density of amines on the surface of the coverslips was quantified using UV absorbance of 4-nitrobenzaldehyde (4-NB, Sigma Aldrich) attached to amines on functionalized coverslips as we have reported elsewhere.[31] Calibration curves of absorbance of 4-NB were generated reading spectra from a UV-Vis Spectrophotometer (Varian Cary 50 Bio) ...
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bioRxiv - Developmental Biology 2021Quote: ... and two 5 min washes in 100% methanol and clearing in 1:1 v/v methanol/BA:BB (2:1 benzyl alcohol:benzyl benzoate; Sigma), and two washes in 100% BA:BB ...
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bioRxiv - Bioengineering 2022Quote: ... the chemostat feed contained 0.25 g L-1 or 2 g L-1 ammonium chloride (equal to 0.07 or 0.9 g L-1 ammonium) (Sigma) for ammonium- or cellobiose-limited cultivations ...