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Citations for Millipore Sigma :
8201 - 8250 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and 25 μL of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane (Sigma 375934). Following derivatization ...
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bioRxiv - Bioengineering 2020Quote: ... They were then resuspended in 1 mL of N-N-Dimethlyformamide (DMF) (≥ 99%, Sigma-Aldrich) and were incubated at room temperature in the dark for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... An equal amount of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilate (Sigma-Aldrich) was added ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was centrifuged at 5,000 g for 5 minutes at 4 °C and the supernatant was added to ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich), equilibrated with the wash buffer (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... The nuclei were stained with DAPI (1mg/ml for 5 minutes) and the coverslips were mounted on glass slides in Mowiol® 4-88 (Sigma) mounting medium containing 1mg/ml p-phenylenediamine (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... The flies were then transferred into vials containing Drosophila instant food (Formula 4-24 Instant Drosophila Medium, Carolina Biological Supply Company, USA) containing 5% H2O2 (Sigma, H1009), or vials containing Drosophila instant food only as a control ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS (1x PBS, 5 min) and then fixed in 4% PFA (Paraformaldehyde, Sigma Aldrich, Dorset, UK) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... female mice between 4 and 16 weeks old were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Sigma, G4877) and 48 hours later ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were incubated overnight at 4°C in TBS Tween (TBST) plus 5% powdered milk and probed with either anti-DNP (Millipore; S7150) or anti-β-actin (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were applied overnight at 4 °C.” Sections were washed in 0.1M PBS and blocked with 5% Chemiblocker (Millipore, Darmstadt,Germany) comprising 0.5% Triton X-100 and 0.05% NaN3 in PBS at pH=7.4 ...
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bioRxiv - Genetics 2020Quote: ... scales were mounted on a microscope slide containing 10 % (w/v) Mowiol® 4-88 (Sigma-Aldrich cat# 9002-89-5) in 25% (v/v ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: PKO cells transfected with Flag-tagged OGT before cultured in 5 mM glucose were collected and incubated overnight at 4°C with ANTI-FLAG M2 affinity gel (Sigma, A2220). Immunoprecipitated complexes were eluted and stained with Coomassie blue ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cultured for one week in 4 ml YNB media with a final concentration of 5 mM Nicotinamide (Sigma I17451). For copper treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... alongside a topical treatment for the tail skin (tails were dipped for 5 seconds in 25 mg/ml 4-hydroxytamoxifen (Sigma H6278) in DMSO) ...
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bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Cell Biology 2022Quote: ... followed immediately by an equal volume of a freshly-prepared 5 mg/mL solution of 4-hydroxy-α-cyano-cinnamic acid (Sigma) in 50% aqueous (v:v ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... The fused embryos were treated with 5 μmol/L ionomycin for 4 min and then incubated in mSOF medium (Sigma, USA) containing 2 mmol/L 6-Dimethylaminopurine(6-DMAP ...
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bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Neuroscience 2024Quote: Mice aged 3 to 5 months were deeply anesthetized and transcardially perfused with 50mL PBS followed by 50mL 4% (w/v) paraformaldehyde (Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Pathology 2023Quote: ... Freshly resected left ventricular tissue (∼2.5-4 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 µg/mL, Sigma, 47036) 30 min at 40C in BIOPS buffer containing the following ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed in 1X PBS for 5 min and coverslipped with mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma, 2370).
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bioRxiv - Microbiology 2023Quote: HLCs were infected at day 18 of the differentiation protocol at an MOI of 5 infectious units (IU) in the presence of 4% polyethylene glycol 8000 (PEG, Sigma-Aldrich) and 1.5% DMSO (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were exposed for six hours to virus with an MOI (multiplicity of infection) of 5 viral particles/cell in the presence of 4 μg/mL polybrene (Sigma-Aldrich). Cells were then washed ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 4-hour window by magnet-purifying schizonts and then incubated with uninfected erythrocytes for 4 hours before treatment with 5% D-sorbitol (Sigma S7900).
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bioRxiv - Biochemistry 2024Quote: ... Actin was then cleaved off the beads overnight at 4 °C using 5 μg/ml TLCK treated chymotrypsin (SIGMA: C3142-25MG) which was then inactivated by 1mM PMSF ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed epithelial cell removal solution (HBSS supplemented with 4% iHS [Sigma] and 5 mM EDTA [Sigma]). The remaining tissues containing lamina propria and muscularis layer were digested for 20 min digestion ...
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bioRxiv - Genetics 2024Quote: ... Laemmli Buffer (100 mM Tris pH 6.8, 20% glycerol, and 4% SDS (Carl Roth, Germany)) and 5% β-mercaptoethanol (Sigma-Aldrich) were added to the samples after which they were heated to 95°C for 10 minutes.
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bioRxiv - Molecular Biology 2024Quote: ... cells were pipetted onto a pad of 2% agarose prepared in YES media with 0.1 mM N-propyl gallate (Sigma-Aldrich, catalog no. P-3130) and 1% gelatin (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ml of 2% L-cysteine solution (2 g L-cysteine (Sigma Aldrich, #168149-100G) in 100 ml 0.1% MMR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 150 μL 2× Laemmli SDS-PAGE loading buffer supplemented with 10% 2-mercaptoethanol (Sigma-Aldrich) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-HG production was assessed via D-2-Hydroxyglutarate (D2HG) Assay Kit (Sigma-Aldrich, USA). Paired GBM cell lines were also treated with hit inhibitors individually and in combination ...
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bioRxiv - Bioengineering 2021Quote: ... We added 2-amino-thioxanthone (17.2 μmol, 2 equ) in dimethylsulfoxide (DMSO, 0.1 mL, Sigma) to a solution of PA-Rho (8.6 μmol ...
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bioRxiv - Molecular Biology 2020Quote: ... Transduced organoids were selected with 2 rounds of 2 μg/ml of puromycin (Sigma-Aldrich) on day 2 and day 4 ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated with the 0.1mM substrate methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (Sigma) for 1h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then pre-treated with 2mM 2-deoxy-D-glucose (2-DG) (Sigma-D6134) for 15 minutes prior to the assay ...
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bioRxiv - Developmental Biology 2022Quote: ... 2.5 μM Oligomycin A (BioShop) or 50 mM 2-Deoxy-D-glucose (2-DG, Sigma) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... these cells were pre-treated or not with 2-bromopalmitate (2-BP, Sigma, 21604-1G) at 50 μM for 30 min ...
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bioRxiv - Physiology 2023Quote: ... For sectioned tissues (Figure 2, Supplementary Figure 2) samples were incubated in 30% sucrose (Sigma) overnight (for brains 5 days) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Triplicates of bacterial overnight cultures in Mueller-Hinton broth 2 (MHB 2, Sigma-Aldrich, #90922) were prepared from three different colonies the day before and grown shaking at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Then washed for 5 min x 3 times and blocked in 1% BSA (A3059, Sigma-Aldrich) (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-AGTTTCTCTGTCCGATTTAAA-3’] are in the plasmid backbone pLKO.1-CMV-tGFP and were from Sigma Millipore (St Louis ...
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bioRxiv - Neuroscience 2019Quote: ... washed 3 x 5 min in PBS and quenched with 1% sodium borohydride (Sigma Cat# 452882) in PBS for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Permeabilization was enhanced by incubation in 0.4% Triton-X in PBS followed by incubation in primary antibodies in PBS containing 0.2% Triton-X overnight at 4 °C (CtB 1°: 1:500, List Biological Laboratories #703; NeuN 1°: 1:500, EMD Millipore, MAB377). The tissue was then incubated with secondary antibodies for 2 h at room temperature (1:250 ...