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Citations for Millipore Sigma :
8351 - 8400 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 1 nL of 12.5 ng/µL of sgRNA and 1 nL of Cas9 protein (300 ng/µL)64 along with phenol red (Sigma Aldrich) were co-injected in one-cell stage embryos ...
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bioRxiv - Immunology 2024Quote: ... and the lamina propria tissues were digested in 5 mL of enzyme cocktail containing 1 mg/mL Collagenase VIII (Sigma C2139) and 10 μg/mL Dnase I (D5025 ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5% (w/v) sodium deoxycholate and 1% (v/v) Triton X-100] complemented with 10 nM sodium azide (S2002, Sigma-Aldrich), 10 mM sodium ascorbate (A4034 ...
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bioRxiv - Genetics 2023Quote: ... cells were blocked for 1 hour at room temperature using PBS 1X buffer supplemented with 5% Normal Goat Serum (Sigma Aldrich), 3% Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were counted again and 100,000 cells were seeded onto 5 μg/mL Humanplasma Fibronectin (Sigma Aldrich,1 mg/mL) in PBS coated (1 hour at 37℃ ...
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bioRxiv - Microbiology 2023Quote: ... A 500 μL aliquot was collected every 30 s or 45 s and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Immunology 2023Quote: ... After infusion of the pancreas via the common bile duct with 5 ml of collagenase 1 mg/ml (type XI, Sigma C7657), they were removed and placed in RPMI 1640 supplemented with 2 ml of collagenase solution and digested for 7 min using a 37°C water bath ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-AGO1 proteins were detected with anti-FLAG-HRP (1:4000 [Sigma] in 5 % milk in PBS-T [0.05% tween20]) or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... each slide was prepared with 9 μL of Galleria homogenate to which 1 μL calcofluor white stain was added (calcofluor white M2R, 10 mg/L, Evans blue, 5 mg/L, Sigma Aldrich). Images were taken with a Leica M80 Stereomicroscope at a 60X magnification ...
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bioRxiv - Biophysics 2023Quote: ... We blocked the membrane for 1 hour with blocking buffer: 5% bovine serum albumin (BSA, BioShop) in tris-buffered saline (BioShop) with Tween 20 (Millipore Sigma) (TBST ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
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bioRxiv - Microbiology 2023Quote: ... was added for 1 hour at room temperature and quenched with 8 µl of 5% (v/v) of hydroxylamine in water (Sigma Aldrich). 1 μl of 200 mM TCEP was added for 1 hour at 55 °C and free thiol groups were alkylated with 1 μl of 380 mM Iodoacetamide (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos and larvae were raised until 5 days post fertilization (dpf) in E3 medium containing methylene blue or in 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) if prevention of pigment formation was required ...
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bioRxiv - Neuroscience 2023Quote: ... preparations were washed for 1 hour in blocking buffer containing 5% (vol/vol) normal goat serum (NGS) (Cat# G9023, Sigma-Aldrich) in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells where thrice washed in PBS then incubated in blocking buffer (5% Donkey Serum (Jackson Immuno Research Labs 017-000-121, 1% BSA (Sigma A9647), 0.1% Triton X-100 (Sigma T8787 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were subsequently blocked for 1 hour at room temperature in blocking buffer (PBS with 5% skimmed milk powder (Sigma-Aldrich) and 0.1% Tween) ...
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bioRxiv - Developmental Biology 2023Quote: ES-E14TG2a were grown in plastic tissue-culture flasks coated with 5 μg/ml plasma fibronectin (F0895, 1 mg/ml, Sigma-Aldrich) in Dulbecco′s phosphate-buffered saline (DPBS with calcium and magnesium ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in binding assay buffer (50 mM Tris HCl, 5 mM MgCl2, 1 mM CaCl2, 100 mM NaCl and 0.1% fatty acid free BSA, Sigma, pH 7.4). For the assay ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl of resuspended RNA was incubated with 1 µl of 10 mM 5′ FAM labelled fluorescent oligonucleotide primer (ICRES nt positions 318–337) (Sigma-Aldrich) and 6 µl ddH2O at 85°C for 1 min ...
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bioRxiv - Microbiology 2023Quote: ... Βecton Dickinson Company) broth or agar plates or TY medium (48) in the presence of 1-5 µg/mL thiamphenicol (Sigma-Aldrich) or anhydrotetracycline (ATc ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Bioengineering 2023Quote: ... centered at each tumoroid were collected and the matrix between cells lysed with Liberase (5 mg mL-1, #5401135001, Sigma Aldrich) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1-hour blocking of non-specific binding sites with PBS with 5%v/v bovine serum albumin (BSA) (Sigma Aldrich) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Female mice (n=5-7 per group) were fasted overnight and then injected with poloxamer 407 intraperitoneally (1 g/kg BW) (Sigma, 16758). Plasma TG concentrations were measured at 0 ...
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bioRxiv - Bioengineering 2023Quote: ... K562 or Jurkat T cells were seeded into a 24-well plate at 2.5 × 105 cells/well in 1 ml cRPMI and transduced in the presence of protamine sulfate (8 μg/ml, Sigma-Aldrich). Transgene expression was assessed 72 hours post-transduction by flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with homemade anti-RBM20 (1:400) primary antibody in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich] ...
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bioRxiv - Biophysics 2022Quote: ... of each IgG were prepared by using ~ 500 μl of antibody (mAb) (1-5 mg/ml) was mixed with 1200 μl of 100mM Sodium Acetate (sodium acetate anhydrous from Sigma Aldrich) having 1mM Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA for electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... Incubation of the primary antibodies was followed by three 15-minute washes with PBT and subsequent incubation with Alexa Fluor-conjugated secondary antibodies (1:500 in 5%NGS) and DAPI (# D9542; Millipore Sigma) (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... serial dilutions of monoclonal antibodies in duplicate were prepared in a 96-well microtiter plate and pre-incubated with pseudovirus for 1 h at 37 °C in the presence of a final concentration of 5 mg/mL polybrene (EMD Millipore), before the pseudovirus-mAb mixtures were added to 293T-hACE2 monolayers ...
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bioRxiv - Biochemistry 2023Quote: ... 1% sodium dodecyl sulfate (SDS), 1% Triton X-100 (Muinao et al., 2018)) with 5 μL/mL of protease inhibitor mixture (Sigma-Aldrich), and 1 μL/mL Benzonase Nuclease (250 U/μL ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing 3x in ddH20 slides were blocked for 1 hour with blocking solution: 5% normal donkey serum (Sigma, Cat#D9663), 0.1% Tween-20 in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked with 5% nonfat dry milk in Tris-buffered saline for 1 h and incubated with primary antibodies for HTT (MAB2166, Sigma-Aldrich) for 12 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the liquid culture phase was collected as a starter culture and diluted 1:100 in fresh BSFB (final volume of 2 L) supplemented with 5 µg/mL vancomycin (Sigma-Aldrich), and incubated on a shaker platform in a humidified ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed with PBS for 5 minutes and blocked at room temperature in 1% bovine serum albumin (BSA, Sigma-Aldrich), 0.5% Triton X-100 (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... This was done from an aqueous solution containing sodium polystyrene sulfonate (NaPSS, 5 mg mL−1) and 3,4-ethylenedioxythiophene monomers (EDOT, 0.01 M) (Sigma Aldrich, MO, USA). The electropolymerization was done with a high-precision potentiostat/galvanostat (PGSTAT204 ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown ∼3.5 hours to OD ∼0.3 and cultures were induced for oligo recombineering with 1 mM m-toluic acid (Sigma Aldrich #T36609) (1 M stock in 100% ethanol) ...
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bioRxiv - Immunology 2023Quote: ... female and male mice received 5 µl of Complete Freund’s adjuvant (CFA, 1 mg/ml, F5881, Sigma-Aldrich, St. Louis, MO) in the plantar skin of hind paw ...
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bioRxiv - Developmental Biology 2023Quote: ... predifferentiated cells were then treated for additional 5 days with differentiation medium: EGF-free medium containing 1 μg/mL prolactin (PRL) (Sigma-Aldrich) and 100 nM dexamethasone (DXM ...