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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
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bioRxiv - Bioengineering 2022Quote: ... and 10x DMEM (First Link Ltd., UK) were mixed at a 4:1 volume ratio and neutralized with 5 M NaOH (Sigma, Canada). Fast Green (Sigma ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated in a modified reaction cocktail (209 µL PBS, 5 µL CuSO4, 25 µL 1 M L-ascorbic acid (Sigma, A2174), 1.25 µL AlexaFluor 488 dye ...
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bioRxiv - Plant Biology 2019Quote: ... Matrix-assisted laser-desorption ionization time of flight mass spectrometry of the XyG oligosaccharides was recorded with a MALDI/TOF Bruker Reflex III using super-DHB (9:1 mixture of 2,5-dihydroxy-benzoic acid and 2-hydroxy-5- methoxy-benzoic acid; Sigma-Aldrich, sigmaaldrich.com) as matrix.
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bioRxiv - Biophysics 2019Quote: Blots were incubated with primary antibody solutions made in TBS-T with 5% milk for RBM39 (Sigma, Cat #HPA001591, 1:2500) GAPDH (CST ...
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bioRxiv - Genomics 2020Quote: ... C57BL/6 ESCs were transduced with the lentivirus at a multiplicity of infection (MOI) ranging from 0.1 to 1 with 5 μg/μL polybrene (Merck Millipore, Cat# TR1003G). From day three onward (e.g ...
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bioRxiv - Neuroscience 2019Quote: ... odours were diluted 1/5 with mineral oil in 15 ml glass vials (27160-U, Sigma-Aldrich, St. Louis MO, USA).
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bioRxiv - Zoology 2019Quote: ... The blocked specimens were incubated in mixture of rabbit anti-5-HT (S5545, Sigma-Aldrich, St. Louis, USA, diluted 1:1000) and rabbit anti-FMRFamide antibodies (AB15348 ...
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bioRxiv - Physiology 2020Quote: ... they were incubated in a humidified atmosphere containing 5% CO2 at 37°C in a chondrogenic medium containing TGF beta-1 (10 ng/ml) (Sigma Aldrich) in DMEM medium supplemented with 10% FBS and 2 mM L-glutamine for up to 2 weeks ...
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bioRxiv - Microbiology 2019Quote: ... (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich), 1% NEAAs and 1% penicillin-streptomycin and maintained at 39 °C in a 5% CO2 atmosphere.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was conducted as follows: 5 ml of a 1 mg/ml sildenafil solution was prepared in PBS (pH 7.4; Sigma Aldrich, UK), then 20 mg of nanoMIL-89 was added to the solution and incubated on a shaker at room temperature for 16-18 hours ...
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bioRxiv - Genetics 2019Quote: ... followed by a 1 hour prehybridization in Hybridization plus (Hyb+) solution (Hyb+ solution contains Hyb- solution plus: 5 mg/ml torula RNA (Sigma-Aldrich), 50 µg/ml heparin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2019Quote: Single cell suspensions of wild type AGM and YS at E9.5 and E11.5 were stained with rat anti-mouse CD144-efluor660 1:200 (eBioscience #50-1441-82, clone eBIOBV13) and 7AAD (Sigma, #A9400). Cells were analyzed and sorted on FACSAria (BD) ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: Cells growing in log phase were harvested for protein extraction using the protein extraction buffer (50 mM Tris–HCl, 150 mM NaCl, 1% Triton-X, 10% glycerol, 5 mM EDTA; all Sigma-Aldrich) and 0.5 μl protease inhibitor mix (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... The clock reporter construct was targeted to the csr-1 locus and selected on resistance to 5 μg/ml cyclosporine A (Sigma # 30024) (Bardiya and Shiu ...
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bioRxiv - Neuroscience 2020Quote: ... Every fourth section was mounted on slides and stained for Nissl with modified Giemsa solution (Sigma-Aldridge, USA; 1:5 dilution). The left drawing in Figure 1A shows the location of the right hindlimb representation area on the rat brain surface (adapted from (Frost et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... spin labels were added to 200 µM final concentration into the same buffer containing 1- or 5 mM L-ascorbic acid (Sigma Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... Twenty μL of protein-compound mix was aliquoted per qPCR tube (Labtidebiotech, P01-0803E) followed by the addition of 5 μL of 1:400 diluted SYPRO Orange (Sigma, S5692). After incubation on ice for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Eyes were enucleated from euthanized mice (sodium pentobarbital, 150 mg kg−1) and dissected in carboxygenated (95% O2 and 5% CO2) Ames’ medium (A1420, Sigma-Aldrich) under dim red light ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0.2% BSA three times for 5 min each and incubated with 1 μg/ml DAPI (Bisbenzimide Hoechst 33342 trihydrochloride, Sigma B2261) and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated in DMEM supplemented with 5% FBS in the presence of a stress agent or vehicle: 1 μM thapsigargin (TG, Sigma-Aldrich), 50 nM halofuginone (HF ...
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bioRxiv - Microbiology 2021Quote: HAE mucus was allowed to accumulate for one week prior to the application of 5 μl of 2 μm red-fluorescent (Cy3) polystyrene microspheres (1:50 dilution, Sigma-Aldrich) to the apical chamber of the transwell ...
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bioRxiv - Neuroscience 2020Quote: ... odours were diluted 1:5 with mineral oil in 15 ml glass vials (27160-U, Sigma-Aldrich, St. Louis MO, USA).
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bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
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bioRxiv - Immunology 2021Quote: Peyer’s patches and mesenteric lymph nodes were digested in digestion media (RPMI, 5% heat inactivated FBS, 10 mM HEPES, 1% P/S, 50 ug/mL DNase I (Sigma DN25), and 0.5 mg/mL Collagenase IV (Worthington Biochemical LS004189) ...
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bioRxiv - Cancer Biology 2020Quote: ... It was mechanically dissociated using a scalpel in 1 ml of lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115] ...
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bioRxiv - Neuroscience 2020Quote: ... Cell free supernatant was filtered through a 0.22 μm filter gently and slowly (5 ml of supernatant passed through the filter over approximately 1 min) (Millipore Sigma, SLGS033SS) for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for one hour at RT ...
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bioRxiv - Biochemistry 2022Quote: ... in PBS for 1 hour at room temperature before probing with anti-Hs haemoglobin (rabbit IgG, 5 µg/mL, Sigma Aldrich), anti-HA (mouse monoclonal Clone HA-7 ...
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bioRxiv - Cell Biology 2022Quote: ... Collected blebs were then pelleted with a centrifugation at 16100 g for 5 min and incubated in a solution containing an exogeneous ATP regeneration system (energy mix) and alpha-toxin to permeabilise the bleb membrane (5% A-Hemolysin alphatoxin (1 mg/mL; H9395, Sigma Aldrich), 2% energy mix (50 mg/mL UTP ...
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bioRxiv - Immunology 2022Quote: ... 2 × 105 MOs were stimulated for 24 h at 37 °C and 5% CO2 with 1 μg/mL LPS (Escherichia coli 0127:B8, Sigma-Aldrich). After LPS elimination ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were treated for 2 h with 100 μM PALA and then lysed in NP40 Lysis Buffer (10 mM HEPES ph 7.4, 142 mM KCL, 5 mM MgCl2, 1 mM EGTA, 0.2% NP-40, and 1X Sigma protease inhibitor cocktail). Lysates were precleared with protein G-sepharose 4B beads (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... if fixed in 95% MeOH/5% GAA washes were done with PBDT (1XPBS/1%BSA/2%DMSO [dimethyl sulfoxide, Sigma Aldrich D8418]/0.5%Tween ...
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bioRxiv - Cancer Biology 2022Quote: ... for 48 h (after that time, fresh media was added), palbociclib (1 μM) (PD033299, #S1116 Absource Diagnostic) or nutlin-3A (5 μM) (Sigma, #SML0580), as indicated ...
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bioRxiv - Immunology 2022Quote: ... Approximately 1 million effector cells and target cells were both spun down at 400 × g for 5 min followed by labeling with 1 μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) respectively according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... difficile strains were routinely grown in BHIS broth or on BHIS agar supplemented with 2-5 μg ml-1 thiamphenicol (Sigma-Aldrich) as needed [58] ...
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bioRxiv - Neuroscience 2022Quote: ... United States) according to the manufacturer’s instructions.\Individual assays were performed for total tau (Tau-5, # 577801, Millipore, Australia, 1:50), h-tau (p-Ser202/Thr205-tau (AT8) ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated in the dark for 30min with SDS buffer (100 mM Tris–HCl pH 8, 1% SDS, 250 mM NaCl, 5 mM EDTA) with 40 mM iodoacetamide (Sigma, #I1149). The resin was then resuspended in SDS buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were ground in extraction buffer (20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, % Triton X-100, 0.1% SDS, 5 mM DTT, 10X Sigma protease inhibitor mixture) and centrifuged at 20,000 x g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were incubated at 37°C for 5 min before addition of 450 µl phenol/chloroform/isoamylalcohol (25:25:1, Sigma-Aldrich) and mixing by rotation ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM Tris, 30 mM MgCl2, 1 tablet of Pierce Protease Inhibitor Mini Tablets, EDTA-Free [Thermo Scientific], 5 U Benzonase [Novagen]), suspensions were incubated with gentle mixing for 30 min at room temperature to degrade DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% non-fat milk and incubated with the following antibodies: anti-FLAG (1:1000, Cat. #F1804; Sigma-Aldrich), and anti-HA (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/ml-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich) to the cell surface ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then blocked for 1h in PBST with 5% of Bovine Serum Albumin (BSA) and incubated overnight with rabbit anti-TH antibody (1:1000, Millipore, AB152) diluted in PBST with 2% of BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Three clones with a confirmed indel and two control clones without indels were and expanded to 30 mL media before they were frozen down at 1 × 107 cells per vial in spent CD-CHO medium with 5 % DMSO (Sigma-Aldrich).