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Citations for Millipore Sigma :
8301 - 8350 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The liver tissues were dissociated using scissors and incubated in a solution of 5 ml PBS containing 1 mg/ml collagenase D (Millipore, 11088858001), 0.5 mg/ml dispase (Millipore ...
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bioRxiv - Pathology 2022Quote: ... with Dulbecco’s minimum essential medium supplemented with 1% penicillin/streptomycin solution and 5% commercial gamma-irradiated fetal bovine serum (Sigma-Aldrich, USA). Cultures were kept at 37°C in an atmosphere with 5% CO2 and observed daily under inverted microscope to search for cytopathic effect ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were stained using DAPI (1:1000 dilution of 5 mg/ml stock made in H2O, Sigma, cat #32670-5MG-F). Following washes ...
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bioRxiv - Microbiology 2019Quote: ... bacterial suspensions corresponding to 1·0 McFarland turbidity were applied on BHI agar supplemented with 0·5% yeast extract (Sigma-Aldrich) and Clostridium difficile Selective Supplement (CDSS ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mM EDTA, 150 mM NaCl, 2.5% SDS, 10 mM N-ethylmaleimide, 5 μl.ml−1 protease inhibitor [P9599, Sigma-Aldrich], 100mg PVPP). Samples were mixed gently for 10 minutes at room temperature and centrifuged at 16,000 xg for 1 minute ...
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bioRxiv - Neuroscience 2019Quote: ... Eyes were enucleated from euthanized mice (sodium pentobarbital, 150 mg kg−1) and dissected in carboxygenated (95% O2 and 5% CO2) Ames’ medium (A1420, Sigma-Aldrich). Retinas were mounted ganglion cell down and maintained in contact with the substrate using a 1 mm nylon mesh ...
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bioRxiv - Bioengineering 2019Quote: ... was incubated in 5% milk powder in TBS-t buffer with anti-tubulin primary antibody (1:500, Sigma #T9026, Buchs, Switzerland)) ...
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bioRxiv - Pathology 2020Quote: ... Recombination was induced in TdTomato reporter mice by two 5-day injection series with 1 mg tamoxifen (T5648-1G, Sigma-Aldrich) per day and in other mice by one 5-day injection series with 2 mg tamoxifen per day (which reported higher recombination efficiencies in our studies).
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bioRxiv - Biophysics 2019Quote: ... at a density of 750,000 cells (per 35 mm petri-dish) in plating medium (85% DMEM, 17% M199, 10% Horse serum, 5% FBS and 1% penicillin/streptomycin, all from Sigma Aldrich). 24 hours later the cardiac sympathetic stellate neurons were isolated from litter mates as described previously with some modifications [38 ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The next day the spots were resuspended in 1.5 mL of BHIS and 100 μL was spread onto several BHIS + TAP/D-cycloserine (250 μg mL−1, Millipore Sigma) plates ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then lysed under denaturing conditions using the lysis buffer (40 mM HEPES KOH, pH 7.4, 1% SDS) containing 5 mM N-ethylmaleimide (NEM, Sigma-Aldrich, E1275) to prevent deubiquitination and boiled for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Females were superovulated at 6-8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 300 mM NaCl, 500 μM EDTA, 5 μg/ml DNAse, 1 mg/ml Lysozym plus Protease inhibitor cOmplete tablets from Sigma Aldrich) and lysed by sonication ...
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bioRxiv - Cancer Biology 2020Quote: ... Differentiation of adherent THP-1 macrophages was obtained following 48 hours of treatments with phorbol-merystate-acetate (5 ng/mL, PMA, Sigma Aldrich), as in [29].
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bioRxiv - Genetics 2020Quote: Approximately 5×106 of the harvested infected cells were resuspended in 1 mL of TriPure Isolation Reagent (Sigma Aldrich, cat. 11667157001) and 200 μl of chloroform were added ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml CTAB buffer (100mM Tris pH 7.4, 0.7M NaCl, 10mM EDTA pH 8.0, 1% CTAB (Sigma-Aldrich, St. Louis, MO)) with 1% β-mercaptoethanol was prewarmed to 65°C and then added to the frozen pellet ...
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bioRxiv - Plant Biology 2020Quote: ... and added to the growth medium to obtain different final concentrations (0.1, 1, 5, 10, 20, 50 µM. Lincomycin (Sigma-Aldrich L6004) was added directly to the growth medium to obtain 0.5 mM as described before (Martín et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were subsequently incubated for 1 hr at room temperature in a blocking solution consisting of PBS with 5% Goat Serum (Sigma-Aldrich). Following blocking ...
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bioRxiv - Cell Biology 2021Quote: ... the coupling reaction was stopped by the addition of 7.5 μl of 300 mM glycine and 1 μl of 5% polyethylene glycol with a mean molecular weight of 20,000 (PEG20K, Sigma Aldrich: P5413) in PBS and incubated at RT for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were blocked in 5 % skimmed milk in PBS prior to incubation with primary antibody (anti-APP A8717 1:5000 Sigma Aldrich) at 4°C overnight prior to incubation with anti-rabbit HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained at 37°C and 5% CO2 and the growing media was supplemented with 1% penicillin/streptomycin (Sigma, UK) and 1% L-glutamine (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... the 5’ sequence of the native promoter and amino acids 1-443 of HEH1 were amplified via PCR (KOD polymerase, EMD Millipore) from genomic DNA using primers encoding the restriction sites BamHI and HindIII ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit/mouse HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for 45 minutes at RT ...
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bioRxiv - Genomics 2019Quote: ... The dishes were then transferred to wet ice and 400 ul of lysis buffer (1X polysome buffer (20 mM Tris-Cl pH 7.4, 150 mM NaCl, 5 mM MgCl2, with 1 mM DTT (Sigma Aldrich #43816) and 100 ug/ml cycloheximide added freshly ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Immunology 2020Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/mL-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich,) to the cell surface ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked for one hour at room temperature (%5 nonfat dried milk in %0,2 TBS-T) and probed with anti-desmin antibody (1:1000; Sigma-Aldrich, D8281) overnight at 4°C or anti-lamin B1 antibody (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking with 5% BSA in PBS for 1 hour at 37°C and incubation with an anti single-stranded DNA antibody (Sigma, MAB3034) diluted 1:80 in 5% BSA in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... XL-413) for 1 h followed by inhibitor incubation together with 100 μM 5-chloro-2ʹ-deoxyuridine (CldU; Sigma-Aldrich, Germany) and ...
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bioRxiv - Developmental Biology 2022Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were eluted with 0.2 mg/mL FLAG peptide and 5 mM EGTA and concentrated to 1-5 mg/mL using an Amicon concentrator with a 100 kDa molecular weight (MW) cutoff (Millipore Sigma). Protein samples were purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2022Quote: ... to which a 5 % of fluoro-surfactant was added (1H,1H,2H,2H-Perfluoro-1-octanol, CAS Nr 647-42-7, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... samples were centrifuged at 600xg for 5 minutes and resuspended in FACS buffer (1% BSA (Millipore Sigma, Product No: EM-2930) in calcium/magnesium-free PBS (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibody (see Reagents) diluted 1:1000 in PBST containing 5% BSA and 0.02% sodium azide (Sigma-Aldrich; S2002) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked using 5% nonfat milk and probed with 1:10000 dilution of anti-HA (Sigma-Aldrich, H6908, Lot # 098M4812V).
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bioRxiv - Genomics 2022Quote: ... females were selected by maturity status and induced to ovulate with an injection of 5 μg kg-1 of GnRHa (Sigma code L4513, Sigma, Spain), whilst no hormones were used on males ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed twice with cold phosphate-buffer saline (PBS) and total proteins were precipitated in 5% trichloroacetic acid (TCA) with 1 mM Methionine (Met) (Sigma-Aldrich) for 15 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Neuroscience 2022Quote: ... and treated with a papain/DNase I (0.1 M PBS/0.1% BSA/ 25 mM glucose/5% papain/1×DNase I [Sigma, catalog #D5025-15K]) solution for 20 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Cell Biology 2024Quote: Filter extracted merozoites were added to uninfected RBCs at 1% haematocrit in media supplemented with 5 µM GGOH (Sigma Aldrich, G3278) in the presence or absence of the invasion inhibitor heparin (100 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... an approximate equivalent volume was added of HFE oil containing 1H,1H,2H2H-perfluoro-1-octanol (5 g solution Sigma-Aldrich, further diluted 4 times in HFE 7500 oil) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 1X TBST containing 5% milk and probed with a 1:2,000 dilution of monoclonal α-FLAG-HRP (Sigma-Aldrich), 1:5,000 dilution of E ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... isolated mitochondria equivalent to 1 mg protein were mixed with 250 μl methanol before adding 5 mL 0.1M Hydrochloric acid (Sigma, Cat# 1090601000) and 100 µL SPLASH (Avanti Polar Lipids ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was stained with DAPI (1 mg/ml in water) for 5 min at RT and coverslips were mounted in fluoroshield (Sigma-Aldrich).