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Citations for Millipore Sigma :
8151 - 8200 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Bioengineering 2020Quote: ... a gradient was prepared such that a 35% (v/v) 1:1 4-morpholinepropanesulfonic acid (MOPS):Opti-prep (Sigma) solution in HBSS was layered on top of a 20% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... was used in 1:250 concentration to label F-actin before mounting in MOWIOL-DABCO (2.5% 1, 4-Diazabicyclo-octane (DABCO) (Sigma), 10% MOWIOL 4-88 (Sigma) ...
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bioRxiv - Pathology 2022Quote: ... Mice were administered CCl4 (1 ml/kg i.p.; diluted 1:4 in olive oil; Sigma-Aldrich, St. Louis, MO) or vehicle 2×/wk for 4 wks ...
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bioRxiv - Microbiology 2019Quote: ... The MALDI matrix used for the analysis was a 1:1 mixture of recrystallized □-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) (Sigma). The plate was then placed in an oven at 37□ for approximately four hours or until the agar was fully desiccated ...
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bioRxiv - Physiology 2019Quote: ... membranes were further incubated overnight at 4 °C with anti-UCP-1 rabbit polyclonal antibody (1:500, Sigma, U6382), anti-PRDM16 rabbit antibody (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... LIF has been omitted and derived EpiSCs can be passaged 1:4–1:10 every by triturating the colonies into small clumps using 0.5 mg ml–1 collagenase IV (Sigma). To derive mesodermal progenitors utilizing the T-eGFP FoxA2-tagRFP double knock-in reporter cell lines ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBST (1:2,000 for mouse anti-GFAP, Sigma; 1:1,000 for chicken anti-GFAP ...
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bioRxiv - Genetics 2020Quote: ... incubated with primary antibodies overnight at 4°C at 1:1,000 dilution for GLI1 (Cell Signalling) or 1:10,000 dilution for β-actin (Sigma). Secondary HRP-conjugated-anti-mouse antibody was applied at a 1:2,000 dilution for 1hr at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The slides were then mounted in 90% glycerol and 10% phosphate-buffered saline (PBS) containing 1% DABCO (1, 4-diazabicyclo-[2.2.2] octane, Sigma-Aldrich). The images and the sections were analyzed by multi-channel image acquisition tool of Fluovision software (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... EHTs were fixed in ice-cold 4% PFA for 1 hour and stained with anti-FLAG (Sigma, 1:1000), Alexa Fluor 568-conjugated wheat germ agglutinin (Thermo Fisher 1:1000) ...
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bioRxiv - Neuroscience 2021Quote: ... P3 and P6 pups were injected intraperitoneally with 50 mg/kg of EdU (5-ethyl-2-deoxyuridine; Sigma). Brains were harvested 2h after injection and processed for frozen tissue sections ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cultured overnight at 37 °C in 5% CO2 in neurobasal medium supplemented with 2% B27 (Sigma-Aldrich) and containing 50 ng/mL GDNF (PeproTech ...
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bioRxiv - Genetics 2019Quote: ... Seedlings were harvested and ground in liquid nitrogen and total protein was extracted in IP buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 10% glycerol, supplemented with 5 mM DTT, 0.5 mM PMSF, Sigma protease inhibitor cocktail P9599 ...
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bioRxiv - Biochemistry 2021Quote: ... with 10% ADC supplement (5% BSA, 0.85% NaCl, 2% dextrose) and 0.05% tyloxapol or 7H10-ADC agar medium (Sigma) at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Neuroscience 2020Quote: ... (+)-Bicuculline (No. 14340) and DL-2-amino-5-phosphonopentanoinc acid (APV; No. A5282) were obtained from Sigma-Aldrich; 6,7-dinitroquinoxaline-2,3(1H,4H)-dione disodium salt (DNQX ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue was then incubated in CUBIC-1A solution (10% Triton, 5% NNNN-tetrakis (2-HP) ethylenediamine (Sigma, 122262), 10% Urea ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were stimulated with 50 U/ml IL-2 and 5◻μg/ml phytohemagglutinin (PHA; Sigma-Aldrich) for 72 h prior to infection or transduction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with 0.1 mL serially diluted supernatant containing SARS-CoV-2 for 30 minutes at 37°C and 5% CO2 before being left in 1.5mL overlay (DMEM, 2% FBS, 1.2% cellulose microcrystalline (Sigma)) at 37°C and 5% CO2 for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... After a 2 h blocking step in blocking buffer (PBX with 5% normal goat serum, Sigma-Aldrich, G9023) at RT ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2) for 2 min and then the DNA digested with 20U DNaseI (cat #: D4527, Sigma-Aldrich) in 1 mL of digestion buffer for 40 sec at RT ...
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bioRxiv - Genetics 2022Quote: ... Experiments for Figure 5 were as Figure 2 with the following changes: 10 μg/mL tunicamycin (InSolution, Sigma) was used ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 5% SDS supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich). The following antibodies were purchased from Cell Signaling Technology (Danvers) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody in 250 μl of cold antibody buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 2 mM EDTA, 0.1% digitonin, and protease inhibitor cocktails from Sigma) was added to the tubes and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spinesbioRxiv - Neuroscience 2021Quote: ... and in addition of 5′fluoro-2-deoxyuridine + uridine (FUDR) (Sigma; 20 μg and 50 μg/ml, respectively), to block glial proliferation ...
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bioRxiv - Physiology 2022Quote: ... L4 staged worms were picked onto NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma F0503), 50 μg/ml carbenicillin ...
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bioRxiv - Cancer Biology 2019Quote: ... Treatment was followed by pulse-labelling for 20 minutes with 25 μM 5-Chloro-2′-deoxyuridine (CldU, Sigma) and then 20 minutes with 250 μM 5-Iodo-2′-deoxyuridine (IdU ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... β-estradiol was removed at day 5 from Type 2 medium and DMSO (Sigma, #D2650; final concentration = 0.1%) or 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... asynchronously growing subconfluent KB2P1.21 or KB2P3.4 cells were labeled with 30μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20’ ...
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bioRxiv - Neuroscience 2023Quote: ... the larvae were anaesthetized with 0.02% Tricaine for 5-10 minutes and immobilized in 1.5-2% low-melting point agarose (Sigma) in fish water ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Systems Biology 2023Quote: ... Immunofluorescence studies were performed on 5 µm sections fixed in 2% paraformaldehyde (PFA; Millipore Sigma, St. Louisa, MO) for 5 minutes at room temperature (RT).
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bioRxiv - Microbiology 2023Quote: ... VirB or its mutant variants were incubated with 5 mM Tris(2-carboxy-ethyl) phosphine (TCEP; Sigma, USA) for 1 h at room temperature to fully reduce all cysteine residues ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Cancer Biology 2024Quote: ... The treatment group received 2 mg/ml Dox (BelaDox) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich), to induce an in vivo knockdown ...
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bioRxiv - Immunology 2024Quote: One million of purified human CD4+ Tsen was activated with an anti-CD3 antibody coated-plate (0.5μg/ml) plus rhIL-2 (5 ng/ml; 11 011 456 001; Sigma). Forty-eight hours post-activation ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 and reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All media used for compound dilutions contained 20 mM glucose and 2% DMSO (Sigma Cat. #D2650-5×5ml).
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bioRxiv - Molecular Biology 2024Quote: ... Gene edition was induced in mESC lines by 5-day treatment with 2 μg/ml Doxycycline (Sigma, D9891). Cell lines expressing the sgRNA that yielded the best percentage of editing were used for further experiments ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Cancer Biology 2022Quote: ... with 100 μM N,N,N’,N’-tetramethyl-ρ-phenylene diamine (Sigma-Aldrich). Glycolytic and mitochondrial ATP production rates were determined according to the XF Real-Time ATP Rate Assay Kit protocol (Agilent) ...
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bioRxiv - Bioengineering 2024Quote: ... freshly prepared 10% (w/w) N,N,N′,N′-tetramethylethylenediamine (TEMED, Sigma, T7024) and 10% (w/w ...
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bioRxiv - Bioengineering 2023Quote: ... we added 2.6 μL of N,N,N′,N′-tetramethylethylenediamine (TEMED; Sigma, 411019) and 260 μL of the 10% w/v APS solution to the PA solution ...