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Citations for Millipore Sigma :
8201 - 8250 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... and the sample supernatant was filtered using low-protein binding bottle-top filters (Millipore Express Plus 0.22 µm). This filtered supernatant was supplemented with final concentrations of 2 mM EDTA (stock EDTA 200 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on SDS-PAGE gels and transferred to PVDF membranes (Cat. #IPFL00010, Millipore, Billerica, MA, USA). The membranes were blocked by 5% fat-free milk diluted in PBS with 0.1% Tween-20 for 40 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... C-reactive protein (CRP) was measured by an ELISA kit (cat. number CYT294, Millipore Sigma, Saint Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... equal concentrations of cell lysate and media proteins were transfer into 0.45 μm PVDF membrane (Millipore, MA, USA). Further ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then analyzed by western blotting using a primary anti-biotin antibody (1:5,000 dilution; Sigma-Aldrich) and an anti-mouse secondary antibody coupled to peroxidase (1:10,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of the cleared protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl 1.25x protein loading buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were prepared in two volumes (approx.8 ml) of CelLyticP buffer (Sigma-Aldrich, Cat No C2360) with 1 % of a Protease Inhibitor EDTA-free tablet (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein (30 µg per gel lane) was separated using SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with TBST buffer (137m M NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Homogenate of 50 μg was added to a process control of 800 ng of yeast enolase protein (Sigma) which was then reduced with 20 mM DTT and alkylated with 40 mM IAA ...
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bioRxiv - Plant Biology 2021Quote: Proteins were separated in 10% Bis-Tris polyacrylamide gel using MOPS buffer and transferred to nitrocellulose membrane (Sigma) for immunoblot analysis ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Microbiology 2021Quote: ... the separated proteins were transferred to nitrocellulose and probed with commercial primary antibodies recognizing FLAG (Sigma, cat# F3165), HA (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Input lysates and pulldown proteins were analyzed by western blotting with the anti-FLAG M2 monoclonal antibody (Sigma) and anti-GAPDH monoclonal antibody (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... Protein extracts were separated by SDS-PAGE and analyzed by western blot with anti-FLAG (Sigma-Aldrich #F7425), anti-DNAk (Abcam #ab69617) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Ni-NTA eluted proteins were incubated with washed/pre-conjugated anti-FLAG magnetic beads (Millipore™ M8823) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted twice with equal bead volume (200 μl) of AP buffer supplemented with 3XFLAG peptide (Sigma) at 200 μg/ml at room temperature for 15 minutes on a Nutator.
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein A/G agarose (Millipore, 100 μL/IP). Following dilution in IP buffer (10 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μg of protein extract per sample was loaded on acrylamide gel and transferred to PVDF membrane (Millipore). All lysis buffers contained a cocktail of protease inhibitors and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2020Quote: ... protein samples were diluted 1:1 in EPPS buffer (10 mM EPPS [Sigma-Aldrich, E9502], 1 mM CaCl2), and digested with 1 µg trypsin (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, Cat. No. I6758) and occurred overnight at room temperature under shaking ...
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bioRxiv - Biophysics 2021Quote: ... The protein solution was then concentrated using a 3 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, USA) and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... protein purification was accomplished by their affinity to Nickel-NTA agarose beads employing the standard protocol (Novagen, Inc.). There is no evidence that the N-terminal Histidine influences the measurements ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were transferred to Eppendorf protein LoBind tubes and stained with DAPI (1 μg/mL, Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... and grown at 37°C to an optical density (600 nm) of ~0.6-0.8 in Super Broth before protein expression was induced with 1 mM IPTG (Sigma) overnight at 18°C ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1 µg/µl total protein using an ANTI-FLAG M2 Affinity Agarose Gel (Sigma). Beads were centrifuged and washed three times in lysis buffer and eluted and denatured (95 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to PVDF membranes and probed with M2 anti-FLAG antibody (Sigma; 1 in 5,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Cell Biology 2022Quote: Purified GFP-Cdt1 protein (1.6 mg ml-1) was treated with CDK1-CyclinB Kinase (EMD Millipore, # 14-450) supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... and the retained lysate was quantified for protein concentration using the bicinchoninic acid (BCA; Sigma, St Louis, Missouri) assay ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and immunoblotted for protein expression using the following antibodies: guinea pig anti-GLT1 at 1:25,000 (Millipore AB1783) and mouse anti-beta actin at 1:20,000 (Sigma-Aldrich A1978) ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 µg retina protein extracts were separated on 8% polyacrylamide gels and blotted onto PVDF transfer membranes (Millipore) followed by blocking with non-fat dried milk (Applichem) ...
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bioRxiv - Biochemistry 2022Quote: ... The dual-affinity purified protein was concentrated via ultrafiltration using an Amicon Ultra-4 30 K device (Millipore), supplemented with BSA and glycerol to obtain final concentrations of 100 µg/mL and 50% (v/v) ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then buffer-exchanged into 50 mM HEPES (pH 7.5) using an ultrafiltration device (Millipore, UFC901096). The Trx-FER-KD and FER-KDK565R proteins (1 μM ...
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bioRxiv - Immunology 2022Quote: ... The protein elute was collected and concentrated using an Amicon Ultra-4 Centrifugal Filter Unit (Millipore Sigma, UFC803024), and further purified via size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Near equal loading of total protein was estimated using the Bradford reagent (Sigma-Aldrich, Saint Louis, MO, USA) with BSA (bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration in each fraction was estimated using the Bradford reagent (Sigma-Aldrich, Saint Louis, MO, USA) with BSA (bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was concentrated by centrifugation using an Amicon Ultra filter (30,000 Mw cut-off; Millipore, USA). The sample media was then replaced with Buffer A (50 mM Tris–HCl ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins of interest were visualized with the Amersham ECL Detection Reagents (GE Healthcare by SIGMA, Cat. No. RPN2105) or LiteAblot TURBO Extra-Sensitive Chemioluminescent Substrate (EuroClone ...
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bioRxiv - Immunology 2022Quote: ... Then 10 μl of pre-washed Magna ChIP™ Protein A+G Magnetic Beads (16-663, Millipore, USA) were added to each sample respectively and incubated for 2.5 h ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated proteins were pelleted by centrifugation and the supernatant was mixed with Ni-NTA agarose beads (Novagen) and tumbled for four hours at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the protein of interest were collected and concentrated to around 30-50µM using Amicon Ultra-0.5ml Centrifugal Filters (Millipore) then separated into single-use aliquots ...
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bioRxiv - Microbiology 2022Quote: ... 6 mg of total protein extract was incubated with 40 µL of Anti-FLAG M2 Agarose Beads (Sigma) and beads were washed a total of five times (three times in ice-cold dilution buffer and twice in ice- cold PBS) ...
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bioRxiv - Plant Biology 2022Quote: ... total proteins were separated on 8.5% SDS-PAGE gel and transferred to an immobilon-P (PVDF) membrane (Millipore). Membranes are then saturated with blocking buffer (TBS 1x ...
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bioRxiv - Genetics 2022Quote: ... The protein concentration in the supernatant was estimated by the spectrophotometric Bradford method (Sigma Chemical Co., St Louis). Carnitine-acetyltransferase assay ...