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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Labelled proteins were biotinylated with 20 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described in “Labeling and click chemistry” ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred onto a polyvinylidene difluoride (PVDF) membrane (Sigma). Membranes were blocked in Odyssey blocking buffer (Li-cor Biosciences ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets were resuspended in 200 µL lysis buffer (1X BugBuster® Protein Extraction Reagent, EMD Millipore; 0.025 U/µL Benzonase® Nuclease ...
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bioRxiv - Microbiology 2023Quote: ... the reduced proteins were transferred to a 30 kDa Microcon filter unit (YM-30 filter units, Millipore) and centrifuged at 14,000 × g for 20 min in all consecutive steps ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected by western blotting with antibodies mab2502 (clone 26) and mab1919 (clone 11C1.3) from Sigma.
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were then sterilised by passing the eluate through a 0.2 μm filter (Merck Millipore), then concentrated using a 3kDa Vivaspin 20 centrifugal concentrator column (Sartorius ...
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bioRxiv - Microbiology 2023Quote: Nascent protein synthesis was determined by treating the cells with puromycin (10 μg/ml, P8833, Sigma Aldrich) for 20 min at 37°C [15] ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble protein fraction was incubated with Lysis buffe-equilibrated FLAG beads (Anti-FLAG M2 Agarose, Sigma) for 3 h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble protein fraction was incubated with Buffer C-equilibrated FLAG beads (Anti-FLAG M2 Agarose, Sigma) for 3 h at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Pathology 2023Quote: ... Equal amounts of proteins were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore, Bedford, MA). Non-specific binding was blocked by incubation with TBST (20 mM Tris-Hcl pH7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... FACS proteins were collected from the non-or Dox-treated cells and diluted in RIPA buffer (Millipore). Biotinylated proteins were isolated by streptavidin pull-down (SA-Pd ...
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bioRxiv - Developmental Biology 2023Quote: ... urea was removed by washing the protein suspension three times with 50mM ammonium bicarbonate (Sigma-Aldrich, Spain). Proteins were digested first by Lys-C (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... The separated proteins were transferred in a semi-dry system onto PVDF membranes (Immobilon P, Merck Millipore) using transfer buffer (192 mM glycine ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra centrifugal filter units with 30 kDa MWCO (Millipore, Darmstadt, Germany). Protein concentrations were measured in a Nanodrop spectrophotometer using the theoretical extinction coefficients calculated using the Expasy ProtParam tool ...
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bioRxiv - Biochemistry 2023Quote: ... total protein was extracted and examined via western blots using HRP-conjugated anti-mouse secondary antibody (Sigma). Protein loading was visualized with Amido Black Staining Solution (Sigma).
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bioRxiv - Cancer Biology 2023Quote: ... The ECM fraction was isolated from the frozen tissues using the Compartment Protein Extraction Kit (Millipore, #2145) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were incubated with 20 µl protein A/G magnetic beads (Sigma Aldrich, 16-663) for 2 hours at 4°C on an end-to-end rotator ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were extracted from residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Metabolite profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under the control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Neuroscience 2023Quote: Cervical spinal cord and cortex protein were extracted using a lysis buffer of RIPA buffer (Sigma; R0278), protease inhibitor (Roche ...
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bioRxiv - Microbiology 2023Quote: ... then protein expression was induced with 0.1 mM IPTG (isopropyl-β-d-thiogalactopyranoside, Sigma Aldrich, cat. PHG000110). Cultures were incubated 3h30 at 25°C under continuous shaking then harvested by centrifugation for 15 minutes at 5000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... PsC23 protein-containing fraction was collected and imidazole was removed by 10K centrifugal filter (Merck Millipore, Germany) using buffer (20 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2023Quote: ... the soluble chromatin was precleared with Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) for 1 hour and then incubated overnight at 4°C with magnetic FLAG-M2 beads or the indicated antibody ...
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bioRxiv - Microbiology 2023Quote: ... 1:200) in the presence of protein A-sepharose beads (20 µl of 50% bead solution, Sigma) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected and transferred to 0.22 μm hydrophilic low protein binding 96-well filter plates (Millipore) and centrifuged at 4000 rpm to remove residual cells ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 8% Bis-Tris gels and transferred onto a 0.45 µm PVDF membrane (Millipore). Membranes were blocked with 5% skim milk powder in TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cas9 protein (CP01, PNAbio) was reconstituted in nuclease-free water and 5% Phenol Red Solution (Sigma Aldrich), following the guidelines in (71) ...
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bioRxiv - Immunology 2023Quote: ... protein samples were dried and reconstituted in 40 µl of 10% formic acid/5% DMSO (Sigma-Aldrich). Samples were analyzed as previously described [Van Herwijnen 2016] with a QExactive Plus instrument (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Proteins were reduced with 10 mM DTT alkylated with 200 mM iodo acetamide (both from Sigma-Aldrich) and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were incubated with 20 µl protein A/G magnetic beads (Sigma Aldrich, 16-663) for 2 hours at 4°C on an end-to-end rotator ...
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bioRxiv - Immunology 2023Quote: ... Briefly, total protein was prepared using RIPA Lysis Buffer (BostonBio Products, Ashland, MA, USA) containing PMSF (Sigma) and quantified with a BCA Protein Assay Kit (Beyotime ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were precipitated by adding an equal volume of ice cold 55% Trichloroacetic acid (TCA, Sigma-Aldrich), incubated 15 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by reducing Tris-glycine SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Merck Millipore) membranes followed by enhanced chemiluminescence analysis (Advansta ...
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bioRxiv - Immunology 2023Quote: ... The protein samples were run on 12% SDS-polyacrylamide gel by electrophoresis and transferred to PVDF (Millipore) or Nitrocellulose (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... Collected protein was incubated 1 hour with 200 µL ANTI-FLAG-M2 affinity resin (Sigma-Aldrich; A2220) pre-washed with 3 times with1.0 mL Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of protein sample was incubated in 57μL sodium acetate buffer pH 5.0 reconstituted in D2O (99.90%, Sigma). Deuterium labelling was performed for 0.5 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM DTT) at varying protein or salt concentrations in the presence or absence of 10% PEG6000 (Sigma) and 10% 1,6-hexanediol ...
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bioRxiv - Cell Biology 2022Quote: ... Fusion protein solution was concentrated using a pre-soaked Amicon® Ultra Centrifugal Filter Unit 50MWCO (Millipore) and concentration estimated with NanoDrop2000 UV-Vis spectrophotometer (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved using SDS-PAGE and transferred onto a activated polyvinylidene difluoride (PVDF) membrane (EMD Millipore) at 400 mA for 3 hours using a MiniTransblot Module (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged proteins were incubated with 30 μl pre-washed anti-FLAG M2 affinity gel (Sigma, #A2220) for 3 h at 4 °C and washed four times with IP wash buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were separated by SDS-PAGE on 12% gels and transferred to PVDF membranes (Immobilon-FL, Millipore). After blocking with for 1 h with blocking buffer (1X TBST with 5% w/v non-fat dry milk) ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to PVDF membranes and immunoblotted for visualization with Immobilon Western chemiluminescent HRP substrate (Millipore) using a GeneGnome XRQ-chemiluminescence imaging system ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... The solubilized proteins were mixed with 20 µL (40 µL slurry) anti-Flag M2 magnetic beads (Sigma) and rolled for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The RafH protein fraction was pooled and concentrated in a 10 kDa cut-off Amicon filter (Millipore). The protein concentration was checked by measuring absorbance at 280 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Millipore Sigma, MO, USA). The membrane was stained with Ponceau S to visualize proteins Membranes were blocked with PBS supplemented with 5% nonfat milk before incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to Immobilon PVDF membranes (EMD Millipore, Billerica, MA, USA). The membrane was incubated for 1 h with antibodies ...