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Citations for Millipore Sigma :
8351 - 8400 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Expression of NK1R-miniGs/q70fusion protein was induced with addition of 4 μg/mL doxycycline hyclate (Sigma Aldrich) and enhanced with 20 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was extracted from 5⨯106 cells with Pierce RIPA buffer (Thermo, 89901) containing proteasome inhibitor (Sigma, P8340) and quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... For protein detection: cell lysates were prepared in RIPA lysis buffer containing protease and phosphates inhibitors (Sigma-Aldrich) and Western blotting was performed as described (69) ...
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bioRxiv - Microbiology 2021Quote: ... The localisation of the codon-optimised NS5 protein was detected with a rabbit-anti-FLAG antibody (Sigma, Germany).
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bioRxiv - Immunology 2020Quote: ... were conjugated to the carrier protein Bovine Serum Albumin (BSA; Applichem or Sigma (low-endotoxin, fatty-acid free)) according to the protocol of Listenberger et al88 ...
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bioRxiv - Microbiology 2019Quote: ... The purified protein was concentrated to 15 mg ml−1 using a centrifugal concentration device (Amicon ultra, Millipore) before storage at 193K ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with rabbit anti-protein VI 26 or mouse anti-E1A clone M73 (Millipore, 05-599) diluted in blocking buffer (10% goat serum in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton 100 and 1 × protein inhibitor) and subsequently precipitated with anti-Flag M2 Magnetic beads (Sigma, M8823) or GFP-Trap (Chromotek ...
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bioRxiv - Immunology 2020Quote: Protein lysates were separated on SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membrane (Millipore, Etobicoke, ON). Membranes were blocked and probed ...
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bioRxiv - Immunology 2020Quote: ... Elastase limited proteolysis experiments were performed in a volume of 300 µL phosphate-citrate buffer pH 7.6 with 90 µg F1-V protein and 0.9 µg of Porcine Elastase (Milipore-Sigma). The reaction mixture was incubated at 37 °C for 1 hour with 20 µL aliquots taken every 5 minutes and dispensed into microcentrifuge tubes containing loading buffer ...
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bioRxiv - Immunology 2021Quote: ... 400 μg protein from the cytosolic lysate was incubated with 5 μg anti-FLAG mouse IgG1 (M2, Sigma) or mouse IgG1 control overnight at 4 °C ...
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bioRxiv - Physiology 2020Quote: ... Subcellular fractions were extracted using differential lysis and solubilization with a commercial Millipore Compartmental Protein Extraction Kit (Millipore) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and the protein was eluted with MES wash buffer supplemented with 500 mM methyl-alpha-D-mannopyranoside (Sigma). The protein was buffer exchanged into phosphate buffered saline by successive rounds of centrifugation and stored at −80°C.
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bioRxiv - Genetics 2020Quote: ... Samples were pre-cleared with BSA-blocked Protein A sepharose beads (cat no. GE17-0780-01, Sigma Aldrich) for four hours at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Target proteins were eluted with 5 column volumes of wash buffer plus 200 μg/mL FLAG peptide (Sigma). The eluent was then applied to Strep-Tactin Sepharose (IBA ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated with a 10% SDS-PAGE (Peqlab) and transferred onto a PVDF membrane (Sigma-Aldrich) via a semi-dry blotter (Peqlab ...
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bioRxiv - Immunology 2022Quote: ... STAT1-CFP and STAT2-YFP fusion proteins were cultured under standard tissue culture conditions in DMEM medium (Sigma) supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by sodiumdodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto a PVDF membrane (Millipore) using the semi-dry transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 hours and subsequently incubated with Dynabeads Protein G and an anti-Flag antibody (Sigma-Aldrich, F1804) at 4°C with rotation for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... To validate successful separation of cell fractions protein levels of PARP1 (nuclear control: Sigma-Aldrich, Cat.-No. HPA045168) and GAPDH (cytoplasmic control ...
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bioRxiv - Molecular Biology 2022Quote: ... Lactate and pyruvate were measured in the protein-depleted flow-through using assay kits (MAK064-1KT, Sigma-Aldrich) and (ab65342 ...
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bioRxiv - Biochemistry 2022Quote: ... Western blotting was performed using a α-4HNE antibody specific to the Michael protein adduct (EMD Millipore Corp.) at a dilution of 1:1000 antibody over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1.5 mg protein lysate was incubated with 150 μl bead bed of pre-equilibrated hemin-agarose (Sigma, #H6390) for SILAC “heavy” group and the same bead bed volume of pre-equilibrated Sepharose (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg of protein lysate was incubated overnight with 30 μL Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant and standards containing bovine serum albumin (BSA) were analyzed using a BCA protein assay kit (Sigma).
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bioRxiv - Bioengineering 2022Quote: ... endotoxin-free protein samples were dialyzed against PBS and filtered through sterile 0.22-μm polyvinylidene difluoride filters (Millipore).
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bioRxiv - Immunology 2022Quote: Mice were subcutaneously immunised in the flank with 1.5 μg of S-protein fragment emulsified in Freund’s Incomplete adjuvant (IFA) (Sigma) containing 50 μg Poly(I:C ...
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bioRxiv - Genetics 2022Quote: ... purified proteins were concentrated and filtered using Amicon ultrafiltration units with a 30-kDa MWCO (Millipore Sigma, UFC903008) and an Ultrafree-MC centrifugal filter (Millipore Sigma ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The equal amount of protein (15μg) was separated by SDS-PAGE and transferred onto PVDF membranes (Millipore USA). After blocking with 5% nonfat milk for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... total proteins were subjected to SDS-PAGE and electrophoretically transferred to a polyvinylidene fluoride membrane (Immobilon-P; Millipore). Antibodies used in the protein blotting analysis were ATG1a (Agrisera ...
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bioRxiv - Microbiology 2022Quote: ... equal amount of protein was resolved on 12% or 10% SDS-PAGE and transferred onto PVDF membranes (Millipore) by semi-dry immunoblotting method (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... samples from overnight cultures were normalized to equal OD600 and resuspended in BugBuster Protein Extraction Reagent (Millipore Sigma) followed by an equal volume of Laemmli Sample Buffer (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The presence of over-expressed protein was confirmed using monoclonal anti-poly histidine alkaline phosphatase antibody (Sigma-Aldrich) at 1:20,000 dilution.
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bioRxiv - Microbiology 2022Quote: ... and the data normalized to total protein content that was determined by the bicinchoninic acid (BCA) assay (Sigma).
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bioRxiv - Plant Biology 2022Quote: ... GST-OsIDD12 and GST-OsIDD13 fusion proteins were expressed in Escherichia coli BL21(DE3) Rosetta strain (Novagen, USA). Frozen cells were extracted with 100 μl of extraction buffer containing 50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Neuroscience 2022Quote: ... and then purified from this culture media using Protein G Sepharose beads (Cat# GE17-0618-01, Millipore Sigma), as described previously (Naito et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% BSA) and pre-cleared with 30 μL of Protein A agarose beads (Millipore Cat No. 16-125) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... hERG channel protein was stably expressed in HEK-293 cells purchased from Millipore (Cat. No. CYL3006; Billerica, MA). Assay methods have been previously described (32–34) ...
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bioRxiv - Immunology 2023Quote: ... The eluted protein was concentrated to 500 μl with a 100-kDa molecular mass cut-off concentrator (Millipore). Concentrated apo-KSHV-GPCR-Gi complex was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Twenty µg of protein samples were loaded and separated on 4–12% Criterion Bis-Tris Gels (Millipore, USA) electrophoresis and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was then eluted in the same buffer that was supplemented with 20 mM maltose (Sigma-Aldrich). The fractions were pooled and concentrated on a Millipore concentrator (WMCO 50 kDa) ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were then analysed by Coomassie staining using Brilliant Blue G colloidal concentrate (Sigma Aldrich, B2025) on 4–12% NuPAGE Bis-Tris gels (Life Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and analyzed by WB using anti-Flag (Sigma-Aldrich F3165; 1:7500), anti-RPL3 (Proteintech 11005-1-AP ...
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bioRxiv - Plant Biology 2022Quote: ... Either total or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using anti-HA (ab18181 ...
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bioRxiv - Systems Biology 2022Quote: The proteins were extracted from the frozen cell pellets by lysing the cells with 1% SDC (Sigma, D6750) in HNN Buffe pH 7.8 (50 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... by adding 20 μL of magnetic beads (Magna ChIP Protein G Magnetic Beads C #16-662, Sigma-Aldrich) and a combination of two anti BMAL1 antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore, Burlington ...