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Citations for Millipore Sigma :
8051 - 8100 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Total protein was extracted from pili-free cell pellets and whole cell lysates using Bugbuster reagent (Novagen) following the manufacturer’s instructions and quantified using a Bradford assay.
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog # 7345) or FLAG M2 resin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The protein samples were resolved by 10% SDS-PAGE and transferred to PVDF membranes (Millipore, Billerica, MA) through standard electroblotting procedures ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were concentrated using centrifugal filters (Amicon Ultra, MWCO 30 kDa and MWCO 10 kDa, Sigma) to 9.96 mg/ml (RTCB ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced by the addition of 125 ng/mL doxycycline hyclate (Sigma-Aldrich #D9891-5G) to cells cultured in complete RPMI 1640 containing tetracycline-approved FBS (Sigma-Aldrich #F0926-50ML).
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bioRxiv - Immunology 2023Quote: ... cells were expanded in triple flasks and protein production induced with doxycycline (0.5 µg/ml, Sigma-Aldrich). Supernatants of confluent cells were harvested every 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins were loaded in the Laemmli sample buffer supplemented with 5% 2-mercaptoethanol (Sigma, catalog #: M3148) to reduce the disulfide bonds ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then treated with 100 µg/mL of the protein synthesis inhibitor cycloheximide (Sigma- Aldrich C4859) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 centrifugal filters (100 kDa cut-off, Merck-Millipore). After treatment with 1 mM diamide for 15 min to completely oxidize the protein ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of protein extract was mixed with 200 μL of UA (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the purified native protein was first concentrated to 8 mg/ml by ultrafiltration (Amicon Ultracel-10K, Millipore), then mixed with 2 mM ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein peak was collected and concentrated using a 10 kDa Amicon Ultra 15 concentrator cell (Millipore), and then applied to a Superdex 200 16/600 pg size exclusion column equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The protease inhibitor cocktail (PIC) used in our protein purifications was purchased from Millipore Sigma (catalog #P2714) and prepared as per the manufacturer’s instructions to generate a 1000X stock.
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bioRxiv - Neuroscience 2023Quote: ... Monoclonal antibodies were purified from the cell culture supernatants by affinity chromatography on Protein G Sepharose (Sigma), using 0.1M glycine ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing monodisperse protein were concentrated in a 100 kDa cut-off Amicon ultra centrifugal filters (Millipore) and flash frozen in liquid nitrogen for further assays ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mg of protein from each sample were incubated with 3 ug of HOXA9 (Millipore # 07-178) or Rabbit IgG Isotype control (Invitrogen # 02-6102 ...
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: Proteins were separated on a by 12% SDS polyacrylamide electrophoresis and transferred to a PVDF membrane (Millipore). Membranes were incubated in 3% skim milk in wash buffer (2 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 4°C and precleared by incubating with a mix of Protein G Plus/A Agarose Beads (Millipore) on a tube rotator for 2-3h ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein lysates for DNA-RNA hybrid pull-down assays were treated with 10 µL RNaseA (Sigma # R6148) or with 10 µL RNaseH (NEB # M0297S ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with a linear gradient of KCl concentrated with a 30,000 MWCO concentrator (Millipore Sigma), and loaded onto a Superdex 200 Increase 10/300 GL and purified using gel filtration buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for detection of proteins by western blot: anti-M2-FLAG (Sigma, F3165), anti-β-tubulin (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were denatured with 4x Laemmli Sample buffer with 10% Beta-Mercaptoethanol (Sigma-Aldrich, cat: #M6250) followed by 10 min boiling at 95℃ ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4C ...
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bioRxiv - Biophysics 2023Quote: ... The protein solution was concentrated using a 30 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, U.S.A.) and loaded into a Superdex200 10/300 GL gel filtration column (G.E ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... The protein expression was induced by addition of 0.1 mM isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) for 6 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The gene encoding the Streptococcus pyogenes M4 protein (uniprot ID P13050) was cloned into pET28_a (+) vector (Novagen). The M4 endogenous N-terminal signal peptide and C-terminal cell wall sorting signal were not included in the construct and a C-terminal hexahistine-tag was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein bands were visualized on X-ray film by electro-chemiluminescence (Immobilon Western HRP Substrate, WBKLS0500, Millipore). For the isolation of chromatin-enriched proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pellets were resuspended in 50 μL of BugBuster Protein Extraction Reagent (Millipore Sigma, SKU 70584-4) and vortexed on Benchmark Scientific Incu-Mixer MP at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The protein sample was concentrated using 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of ∼0.8mg/ml and filtered by 0.22 µm pore size PVDF membrane prior to deposition on the grid for EM imaging.
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The separated proteins were transferred by electrophoresis to Immobilon-P polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA) and treated with diluted antibodies ...
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bioRxiv - Biophysics 2024Quote: NMR experiments were performed on protein in 90% NMR Buffer and 10% deuterated water (99.8%, Sigma-Aldrich). The PHL4effector domain concentration was 73.2 µM for the 13C and 15N labeled sample and 98.2 µM for the 15N labeled sample ...
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bioRxiv - Biophysics 2024Quote: ... The cleaved WW4-HECT protein was concentrated (Amicon Ultra-15 centrifugal filter, 10 kDa cutoff; EMD Millipore), filtered ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound material was recovered after incubation with 15 μL of protein G beads (Millipore, cat # 16-266) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was digested by incubation with benzonase (Sigma, 25 U per expected 1 mg of protein) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: The NSP3(1-110) protein was then immobilized on Anti-Flag M2 Affinity gel (Sigma Aldrich, A2220), and the cell lysates from stable cell line 293T-N ...
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bioRxiv - Biochemistry 2024Quote: ... Bound protein was eluted off agarose gel with 30-50 μL 1X 3XFLAG peptide (Sigma catalog #F4799) at 4 °C with rotation for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... Then 495 µL of the protein sample was mixed with 55 µL 99.8% deuterated water (Sigma-Aldrich). The diffusion NMR NMR samples for the PHL4FL and PHL4effector domain were prepared by concentrating pure non-isotopically labeled ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were collected and concentrated to 500 µL with a 30K MWCO concentrator (EMD Millipore). Concentrated Ndel1 was then purified via SEC on a Superdex200 10/300 column (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... cells were resuspended and lysed in BugBuster® Master Mix Protein Extraction Reagent (Millipore Sigma, Cat# 71456) at room temperature following the manufacturers recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of total protein were separated on 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The resulting membranes were blocked for 1 hour at 37°C with TBST buffer (12 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: For top-down EfpL measurements the proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat. IPVH00010). Membranes were incubated with primary antibodies in 5% BSA in 1x TBST buffer overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C until further use ...