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Citations for Millipore Sigma :
8151 - 8200 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165). Per IP ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibody was detected with α-mouse IgG HRP (HorseRadish Peroxidase)-conjugated antibody (A0168, Sigma), diluted 1:50000 in 2%MPBST ...
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bioRxiv - Microbiology 2022Quote: ... Anti-MptC antibody was previously raised (47) and anti-HA antibody was purchased from Sigma-Aldrich.
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies used in this paper are mouse anti-CMV IE1/2 monoclonal antibody (MAB8131, Millipore), mouse anti-CMV pp52 monoclonal antibody (CH16 ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were anti-Cralbp (Rlbp1; Fisher antibody MA1-813) and anti-Gfap (Millipore Sigma AB5804).
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bioRxiv - Cancer Biology 2022Quote: ... Antibody binding was detected with a secondary antibody coupled to horseradish peroxidase (Sigma, NA934 and NA931) using enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... anti-αvβ3 (14-0519, eBioScience) integrin antibodies or anti-mouse isotype IgG antibody (M6898, Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and goat anti-rabbit polyclonal secondary antibody conjugated to TRITC (all antibodies from Sigma Aldrich, Co). The nuclei were marked with DAPI (4′,6-diamidino-2-phenylindole) ...
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bioRxiv - Immunology 2020Quote: ... antibody buffer exchange was performed by washing purified antibody through a 50 kDa MWCO microfilter (Millipore) and centrifuging for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used: anti-LC3B antibody produced in rabbit (L7543, Sigma Aldrich), anti-SQSTM1/p62 antibody produced in mouse (ab56416 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used: mouse anti-Myc antibody clone 9E10 (13-2500 Sigma-Aldrich), rabbit polyclonal anti-Myc (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and then incubated with the primary antibody (rabbit anti-human lactoferrin antibody; Sigma, Cat-No. L3262) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... with primary antibodies diluted in PBST using anti-acetylated tubulin antibody produced in mouse (Sigma-Aldrich) and a rhodamincoupled phalloidin (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Antibody buffer exchange was performed by washing purified antibody through a 50-kDa MWCO microfilter (Millipore) and centrifuging for 10 min at 12,000g at RT ...
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bioRxiv - Microbiology 2023Quote: ... plates were incubated with a secondary antibody (anti-Human IgG-Phosphatase alkaline antibody (reference: A9544, Sigma)) ...
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bioRxiv - Cancer Biology 2023Quote: ... antibodies followed by an anti-mouse secondary antibody conjugated to alkaline phosphatase (AP) (A3562, SIGMA-Aldrich). We developed the immunoblots with BCIP/NBT color development substrate and normalized protein levels to Ponceau Red staining ...
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bioRxiv - Biochemistry 2023Quote: ... before the secondary antibody was added (anti-mouse IgG-peroxidase antibody produced in goat, Sigma-Aldrich). The secondary antibody was diluted 1:50,000 in 5% skim milk solution in TBS-T and incubated for two hours at room temperature on a slow shaker ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies: ST8Sia2 monoclonal antibody (Catalog WH0008128M1, Sigma-Aldrich, St. Louis, MO, USA) and polySia-specific monoclonal antibody (mouse IgG2a ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then incubated in antibody solution containing anti-FLAG®M2 monoclonal antibody (Sigma F1804) (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary osteoblast precursors were isolated from the calvarias of newborn mice by sequential digestion (0.2 % dispase Grade II (Roche, Sigma-Aldrich, /collagenase Typ Ia ...
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bioRxiv - Molecular Biology 2019Quote: A subset of sample cell populations were analyzed using an Amnis® Imagestream X Mark II (EMD Millipore; Burlington, MA) equipped with 405nm ...
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bioRxiv - Physiology 2022Quote: ... Complete skin was separated from the body and incubated in 2 ml dispase II solution (5 mg/ml in 50 mM HEPES/KOH pH 7.4, 150 mM NaCl; Sigma-Aldrich) over night at 4°C ...
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bioRxiv - Physiology 2019Quote: ... Our Na+-selective microelectrodes were constructed using 100 mM NaCl backfill solution and Na+ ionophore II cocktail A (Sigma Aldrich), while K+ selective microelectrodes contained 100 mM KCl backfill solution and K+ ionophore I cocktail B (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... the pIVEX 1.3 WG vector suitable for cell-free synthesis of the protein with the Rapid Translation System (5PRIME, Hamburg, Germany) and (ii) the bacterial expression vector pET-30a (Novagen). The latter yielded a chimeric TatB construct carrying a combined His-/S-peptide-tag ending with a BrCN cleavage site immediately upstream of the N-terminus of mature TatB (Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... Black Gold staining was conducted according to the manufacturer’s instructions (Black Gold II myelin staining kit, EMD Millipore, Billerica, MA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... lipid-rich AlbuMAX II was replaced by complementing culture media with an equivalent amount of fatty acid free bovine serum albumin (Sigma), 30 μM palmitic acid (C16:0 ...
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bioRxiv - Neuroscience 2020Quote: ... before being rehydrated in milliQ water for 3 min and incubated with preheated 0.3% black-gold II stain (Millipore, AG105) at 60°C for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... A 5% mercury chloride solution was prepared fresh for each experiment day by dissolving Mercury (II) chloride (HgCl2, Sigma-Aldrich) in distilled and de-ionized water (MilliQ water ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). As for the fibroblasts ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
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bioRxiv - Biochemistry 2020Quote: ... ii) storage buffer (10 mM NaCl, 10 mM Tris-HCl) with 0.1 mg Pronase E from Streptomyces grisens (Sigma Aldrich) with 0.5% (v/v) ...
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bioRxiv - Immunology 2021Quote: ... Data was obtained through the Amnis ImageStreamX Mark II imaging flow cytometer and INSPIRE software (EMD Millipore, Billerica, MA, USA). The accompanying IDEAS software (EMD Millipore ...
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bioRxiv - Microbiology 2020Quote: ... The fragments were amplified with Phusion HotStart II DNA polymerase and were cloned into LIC-vector (LIC, ligation independent cloning) pET46EkLIC (Novagen). The ligation products were transformed into NovaBlue competent cells ...
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bioRxiv - Immunology 2020Quote: ... Cells were then plated onto LabTech II 8-well glass bottom chambers modified with 0.1% Poly-L-Lysine (PLL, Sigma P8920) and allowed to adhere for at least 4 hours at 4C before performing confocal microscopy.
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bioRxiv - Microbiology 2022Quote: ... cells were analyzed on Amnis Mark II ImageStream (405 nm, 488 nm, 561 nm, and 642 nm excitation lasers, 40× magnification; EMD-Millipore) and data analysis was performed using IDEAS software (Amnis).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... aliquots of 15 µg of hippocampal protein were extracted with lysis buffer containing phosphatase and protease inhibitors (Cocktail II, Sigma) and protein concentration was determined by Bradford’s method ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The brains from the different above-mentioned groups were homogenized in 200 μL PBS with EDTA 5 mM and complete protease inhibitor cocktail II (Sigma). The homogenates were aliquoted into microtubes for each treatment group and subjected to 7-point temperature gradient per duplicate (room temperature ...
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bioRxiv - Pathology 2020Quote: Mouse primary hepatocytes were isolated by gradient density centrifugation of 30% Percoll solution after liver perfusion using Collagenase II (Sigma). Primary hepatocytes were cultured in William’s E Medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial strains were grown in glass test tubes (18 × 150 mm) at 35°C in cation adjusted Müeller-Hinton Broth II (MHB, Sigma-Aldrich) with agitation at 210 revolutions per minute (RPM) ...
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bioRxiv - Zoology 2020Quote: ... cells were moved and stored on ice and subsequently run on the ImageStream®X Mark II (Amnis Millipore Sigma), where 10,000 nucleated and focused events were imaged for each sample (at least n=4 snail and n=6 fish ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was cut into small pieces by scalpels and was enzymatically digested in a mixture of Collagenase II (CLS2, 1mg/ml, #C6885, Sigma) and DNase I (0.025mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore; endogenous RNA pol II IPs) or GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Cell Biology 2021Quote: ... by digestion in 10mL/1g tissue of Enzyme Digest Solution (1mg/mL Dispase II, 0.5mg/mL Collagenase A and 1.5mg/mL DNAse l (Sigma-Aldrich, USA)) in advanced-DMEM/F12 (Thermofisher ...
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bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... the resin was washed three times in wash buffer (TBS containing 0.1% NP-40 and phosphatase inhibitor cocktails II and III, Sigma-Aldrich). Protein baits were eluted with Strep-elution buffer (2mM desthiobiotin in TBS) ...
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bioRxiv - Cell Biology 2021Quote: ... Small pieces of lung tissues (2 cm x 2 cm) were moved into a conical tube containing 10 ml of digestion buffers (2ml of dispase II (Sigma), 100 µl of collagenase/dispase (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).