Labshake search
Citations for Millipore Sigma :
8101 - 8150 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Coverslips were incubated with primary antibodies diluted in 5 % BSA in 1x PBS: Tau1 clone 46 (1:500) mouse monoclonal IgG (Sigma-Aldrich, T-9450), GFP (H-140 ...
-
bioRxiv - Biophysics 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-UBTF, Novus Biologicals, NBP1-82545; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-Fibrillarin, EMD Millipore, MABE1154 ...
-
bioRxiv - Plant Biology 2022Quote: ... a 1:5000 dilution of anti-rabbit IgG secondary antibodies was employed and detected by chemiluminescence with the Luminata pack (Millipore, Billerica MA, USA).
-
bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
-
bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated for 1 h at room temperature with sheep anti-mouse horseradish peroxidase (HRP)-conjugated antibody (Catalogue no. GENA931, Millipore Sigma, MO, USA) diluted 1:10000 in Milk/PBST ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
-
bioRxiv - Neuroscience 2023Quote: ... eYFP fluorescence was used to confirm TH+ expression in VTA cell bodies using a rabbit anti-TH antibody (1:1000; Millipore Sigma, Burlington, MA) as the primary antibody ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... astrocyte cell bodies were identified by immunolabeling against Glutamine Synthetase using rabbit anti-Glutamine Synthetase antibody (1:500, Sigma-Aldrich catalog# G2781, RRID:AB_259853), and GABA immunoreactivity was detected by immunostaining with guinea pig anti-GABA antibody (1:500 ...
-
bioRxiv - Zoology 2022Quote: ... Detection of smooth muscle was achieved using a mouse monoclonal primary antibody to smooth muscle actin (SMA, Sigma A2547, diluted to 1:600) and a donkey anti-mouse secondary antibody conjugated to the fluorophore Alexa 555 nm (Invitrogen A31570 ...
-
bioRxiv - Bioengineering 2024Quote: ... Washes were followed by the addition of 100 μL of anti-FLAG horseradish peroxidase (HRP)-linked monoclonal antibody (1:10,000 mAb, #A8592, Sigma Aldrich, St. Louis, MO) for 1 h at room temperature with rocking ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
-
bioRxiv - Pathology 2024Quote: ... The membranes were incubated after washing with an anti-rabbit IgG alkaline phosphatase secondary antibody (1:5000, Sigma– Aldrich, St Louis, MO, USA) for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tissues in fixed samples were blocked for 4 hours and then incubated using primary antibodies overnight: αH3P 1:250 (Millipore Cat# 05-817R); 6G10 1:500 (DSHB Cat# 6G10-2C7) ...
-
bioRxiv - Neuroscience 2023Quote: ... the dye hFTAA59 was used at dilution of 1:400 in combination with the Cy3-conjugated monoclonal anti-α-smooth muscle actin antibody (Sigma Aldrich, C6198) at dilution of 1:800.
-
bioRxiv - Genetics 2023Quote: ... Slides were incubated with goat anti-mouse collagen α1(XI) antibody at 1:500 dilution and mouse anti-rat PAX1(Cat #MABE1115M, Sigma-Aldrich, MO, USA), in PBS + 0.1% Triton + 1% normal goat serum at 4L°C overnight ...
-
bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
-
bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
-
bioRxiv - Neuroscience 2023Quote: ... The same procedure was then applied to the same membranes with anti-tubulin antibodies (mouse anti-tubulin, 1:40000, Sigma Aldrich cat. # T16199). The molecular weight ladder corresponds to Invitrogen SeeBlue™ Plus2 (reference LC5925).
-
bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... and probed with the appropriate primary antibody diluted in TBS-T with 3% BSA: rabbit anti-HA (Sigma Aldrich #H6908; 1:3,000 dilution), mouse anti-FLAG (Sigma Aldrich #F1804 ...
-
bioRxiv - Bioengineering 2023Quote: ... The following day the tissues were incubated in primary antibody diluted 1:100 in fresh block buffer and 5% (v/v) dimethyl sulfoxide (DMSO; Sigma; a penetration enhancer) at 37°C for 24 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were then washed with PBST for 5 min and incubated with the following primary antibodies: GAPDH (EMD Millipore, #MAB 374, 1:2000), β-catenin (EMD Millipore ...
-
bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of mouse anti-FLAG antibody (Sigma-Aldrich; F1804-1MG) and incubated at RT for 1 hour ...
-
bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight at 4°C with primary antibody in blocking solution : anti-vinculin (Sigma-Aldrich, catalogue no. V9131, dilution 1:400) followed by three washes with PBS Tween 0.2% ...
-
bioRxiv - Cancer Biology 2023Quote: ... incubated with primary antibodies (anti-dsRNA, clone rJ2; Sigma-Aldrich, cat# MABE1134 and anti-LINE-1 ORF1p, clone 4H1; Sigma-Aldrich, cat# MABC1152) with desired concentration (1:50 ...
-
bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 1h in a blocking solution of 0.01 M PBS with 0.25% Triton-X and 3% normal goat serum (NGS) and then incubated overnight at 4 °C with rabbit anti-NeuN antibody (1:2000, MAB5504, Millipore, Temecula, CA, USA) in 0.01 M PBS with 0.25% Triton-X and 3% NGS ...
-
bioRxiv - Physiology 2024Quote: ... and the membrane was incubated for 2h at RT with rabbit anti-GAPDH antibody (Sigma Aldrich, G9545, diluted 1:2000 in TBS-T) at RT ...
-
bioRxiv - Immunology 2023Quote: ... ELISA signal in each elution sample was checked using 1:5000 diluted goat anti-human IgG (Fab specific) HRP-conjugated secondary antibodies (Sigma-Aldrich, A0293-1ML). Elution fractions showing an ELISA signal were pooled and concentrated under vacuum to a volume of ∼1 μL ...
-
bioRxiv - Genomics 2023Quote: ... The membrane is washed again with 1x TBST solution, and then incubated the primary antibody solution (3% BSA in TBST, 1:250 anti-HA (Sigma-Aldrich, A2095-1ML), 1:500 anti-FLAG (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
-
bioRxiv - Microbiology 2024Quote: ... The membranes were blocked with blocking buffer (TBS containing 0.1% Tween-20 with 5% w/v skim milk) and then incubated with the primary antibody (anti-FLAG M2 (Sigma-Aldrich, USA; #F1804) or anti-alpha-tubulin (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was washed with TBST buffer and the membrane was set for 2 hours at room temperature with the secondary goat anti-rabbit IgG HRP antibodies (Sigma Aldrich; 1:10000) diluted in a TBST buffer with 3% PVP ...
-
bioRxiv - Biophysics 2024Quote: ... 1%DMSO in PBS) and incubated overnight with mouse monoclonal ANTI-FLAG® M2 antibody (Cat. F1804, Sigma-Aldrich, Inc., St Louis, MO) at a concentration of 1:800 in K-block ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissues collected from leptomeninges and peri-infarct cortex are gently minced and then digested at 37°C for 30 min with an enzyme cocktail (Collagenase type IV; Sigma-Aldrich, C5138, DNase I; Sigma-Aldrich, D4263). FACS analysis was performed to determine tdTomato+ and CD271 FITC using BD Fortessa with a no labeled control for determining appropriate gates ...
-
bioRxiv - Microbiology 2024Quote: Peptidoglycan was extracted from bacterial sacculi (wild-type E. faecium, ΔsagA, and ΔsagA/psagA) and digested with mutanolysin from Streptomyces globisporus (Sigma, 10 KU/ml of mutanolysin in ddH2O) as previously described5 ...
-
bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal antibody to IQSec1 (G4798) and mouse monoclonal antibody to vinculin (V9131) were from Sigma. Mouse monoclonal antibody to β1-Integrin (610467 ...
-
bioRxiv - Cell Biology 2020Quote: ... with the appropriate secondary antibody (Anti-mouse IgG phosphatase conjugated antibody (Sigma-Aldrich Cat# A3688, RRID:AB_258106), or Anti-rabbit IgG phosphatase conjugated antibody (Sigma-Aldrich Cat# A3687 ...
-
bioRxiv - Neuroscience 2020Quote: ... antibody-bound beads were prepared using 2 μg rabbit anti-MAP3K13 polyclonal antibody (Sigma-Aldrich, HPA016497) in 800 μl of lysis buffer with 50 μl of 50% Protein G agarose bead slurry ...