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Citations for Millipore Sigma :
8051 - 8100 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... raised against gel-purified ATLP1 using alkaline phosphatase(AP)-conjugated goat anti-rabbit IgG (1:10000 dilution) as the secondary antibody and 5-bromo,4-chloro,3-indolylphosphate(BCIP)/nitroblue tetrazolium (NBT)(Sigma Chemical Co. MS) as the substrates (Safadi et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Microbiology 2021Quote: ... and PilA2-Flag was detected using a horse radish peroxidase (HRP)-coupled primary anti-Flag antibody at a 1:2000 dilution (Sigma, cat. no. SAB4200119). Enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in PBS containing 0.02% Triton X-100 and 2% NDS overnight at 4°C with primary antibodies: anti-ChAT (1:500, goat polyclonal, Millipore Cat# AB144P, RRID:AB_2079751) and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2uL of lysate (approximately 2ug) was spotted directly onto 0.2 um nitrocellulose membranes and blotted with primary antibodies against puromycin (Sigma MABE343 1:25,000 dilution) and vinculin (Abcam ab18058 ...
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bioRxiv - Synthetic Biology 2021Quote: ... non-fat milk in TBST buffer (Tris-buffered saline containing 0.05% Tween-20) overnight at 4 °C and incubated with anti-FLAG antibody (Sigma, F1804, 1:1000 dilution) or anti-HA antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... and probed over-night at 4°C either in TBS-TM with a 1:1000 anti-Flag antibody (Sigma-Aldrich; catalogue No F3165), or with a 1:10000 anti-LpoA antibody (loading control 28 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated overnight at 4°C with the following primary antibodies: mouse anti-DCP2 (Sigma, Saint Louis, MI, USA; 1:20), rabbit anti-LSm4 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... blots were incubated overnight at 4 °C with the corresponding primary antibody: anti-phospho-histone 2AX (Ser139) (EMD Millipore, 05-636, 1:1000), anti-phospho-Akt (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific staining was blocked with blocking solution (TBST and 4% normal donkey or normal goat serum) and incubated overnight at 4°C with SOX9 antibody (1:2000, Millipore, AB5535, RRID: AB_2239761). Sections were washed and incubated with Alexa 568-conjugated secondary antibody (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... subjected to heat induced antigen retrieval in a citrate buffer and incubated with a mouse anti-NeuN antibody (clone A60, Millipore, MAB377, 1:100). The used secondary antibodies were mouse linker antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... for two hours prior to overnight incubation at room temperature with primary antibodies against dopamine-β-hydroxylase (DBH, a marker of noradrenergic neurons: Mouse, 1:500, Millipore, MAB308, UK), mCherry (Rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-β-actin antibody (AC-15; catalog #A5441) utilized for western blot (1:10,000) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Alexa 568-conjugated secondary antibody (catalog# A11031 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the following primary antibodies for one hour at room temperature: mouse anti-α-tubulin (DM1α) 1:500 (Millipore Sigma #T6199), rat anti-tubulin clone YL1/2 1:1500 (Millipore Sigma #MAB1864) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Sigma-Aldrich, Taufkirchen, Germany) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for primary culture neurons (supplier, catalog number and working dilution are indicated): anti-Gad67 ms IgG2a (Millipore MAB5406, 1:500); anti-somatostatin rat (Millipore MAB354 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the incubation of cells with horseradish peroxidase (HRP)-coupled anti-FLAG M2 antibody (dilution-1:5000; Sigma-Aldrich; cat. no. A8592), prepared in 1% BSA for 90min ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Sections of the brainstem that contained CTB labeled neurons or mCherry neurons were incubated in a solution containing a mouse anti-TH monoclonal antibody (1:10,000; T-2928, Sigma-Aldrich, Oakville, ON, Canada) for 2 days ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, Cat No A2179) in PBST ...
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bioRxiv - Microbiology 2022Quote: ... Blot was probed with ∼1:10,000 diluted primary antibody (anti-Flag™ for ParA-M2, F7425 Sigma-Aldrich, and anti-DnaA for DnaA) overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... spinal motor neurons from six sections covering a distance of 640 µm were immunolabelled using a goat anti-choline acetyltransferase (ChAT) antibody (Merk Millipore, AB144P, 1/50) and a biotinylated donkey anti-goat IgG (Jackson ...
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bioRxiv - Immunology 2021Quote: ... Samples were then incubated in secondary antibody conjugates Atto 647N (1 μg/mL each of anti-mouse; Sigma catalog no. 50185-1ML-F) for 60min ...
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bioRxiv - Neuroscience 2020Quote: ... These free-floating sections were blocked with 1% normal goat serum for 1 hour in 0.01M phosphate buffered saline (PBS) before incubation with with a mouse α-NeuN (Fox-3) antibody (1:1000; Millipore, Cat#: MAB377 (2018)) and a rabbit α-TDP-43 antibody (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... cells were treated for approximately 18h with 5 μM 5-Azacytidine to covalently trap Mt2 or AMt2 with its target cellular RNA before RNA immunoprecipitation using an anti-FLAG antibody following manufacturer’s protocols (SAB4301135 - Sigma-Aldrich, 1:100 dilution) (23).
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bioRxiv - Neuroscience 2022Quote: ... for two hours prior to overnight incubation at room temperature with primary antibodies against dopamine-β-hydroxylase (DBH, a marker of noradrenergic neurons: Mouse, 1:500, Millipore, MAB308, UK), mCherry (Rabbit ...
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bioRxiv - Neuroscience 2022Quote: ... sections were blocked for 1 hour in 10% normal goat serum (at room temperature) and probed with an anti-NeuN Alexa Fluor 488 conjugated antibody (1:100; Millipore-Sigma cat. # MAB377X) for 2 hours at room temperature and protected from light ...
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bioRxiv - Genetics 2022Quote: ... For normalization of signals, we used actin or Pgk1, which was detected by using mouse anti-actin monoclonal antibody (1:5000, (Millipore Cat# MAB1501, RRID:AB_2223041)) or mouse anti-Pgk1 antibody (1:10000 ...
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bioRxiv - Neuroscience 2019Quote: Cultured human cortical neurons of 100-120 days post-differentiation were immunostained as above with primary antibodies against MAP2 (Mouse-anti-MAP2, Sigma, 1:200 dilution), VGLUT1 (Mouse-anti-VGLUT ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000, EMD Millipore, #AP132B, RRID:AB_11212148) followed by 1 h with avidin-biotin complex (ABC Vector Laboratories ...
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bioRxiv - Pathology 2020Quote: ... The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA, cat. no. A5228; 1:500; Sigma-Aldrich), anti-vimentin (cat ...
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bioRxiv - Cell Biology 2020Quote: ... Then protein bands were detected by incubating with secondary antibodies for 1-2h at room-temperature and visualized with ECL reagent (Millipore, Billerica, MA, USA) by Chemi Doc XRS+gel imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... We incubated free-floating CeA coronal sections (16 µm) for 2 h at 30 °C with rabbit anti-FG antibody (Millipore; AB153I; 1:200) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were blocked with 5% BSA diluted in PBS for 4 h at 4°C and incubated overnight with pan-Cadherin antibody (Sigma, C1821, 1:500), MRP2 antibody (Sigma ...
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bioRxiv - Genetics 2019Quote: ... hiPSC-CMs were permeabilized with 0.1% Triton X-100 and incubated in 5% normal goat serum before overnight incubation with the primary α-actinin antibody (Sigma Aldrich, A7811) at 4C ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with FACS buffer (2% FBS and 5 mM EDTA in PBS) prior to staining with antibodies and/or Calcofluor White M2R (Sigma, 1 μg/mL) in FACS buffer for 15-20min ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from fresh frozen pulverized postmortem brain tissue using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in PBS and then incubated with fluorescent secondary antibody (Alexa Fluor 488, 1/500, Life Technology) incubated with propidium iodide (Sigma, 30 μg/ml) and then analysed by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...