Labshake search
Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
-
Characterization of pediatric porcine pulmonary valves as a model for tissue engineered heart valvesbioRxiv - Bioengineering 2023Quote: ... The co-stain dye was prepared from a 1:50 dilution of anti-α-SMA CY3 conjugated primary antibody (Sigma, cat #C6198) and 1:50 dilution of anti-vimentin Alexa Fluor™ 488 conjugated primary antibody (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 µl wash buffer containing 10 µl 1 µg/µl anti-rabbit secondary antibody raised in guinea pig (Sigma-Aldrich, SAB3700889) and 1 U/µl RNase inhibitor ...
-
bioRxiv - Molecular Biology 2023Quote: The cardiac sections were processed via the indirect immunofluorescence method using rabbit polyclonal antibodies against tyrosine hydroxylase (TH, Millipore, #AB152; 1:100) to stain adrenergic nerve fibers and growth association protein 43 (GAP-43 ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a 16-h incubation in PBS with 0.1% Triton™ X-100 with the following antibodies: mouse monoclonal anti-NeuN (1:500; EMD Millipore, MAB377), rabbit polyclonal anti-KCC2 (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
-
bioRxiv - Bioengineering 2023Quote: ... The tissues were then washed and stained with 1:100 dilution of mouse anti-sarcomeric α-actinin primary antibody (A7811, Sigma-Aldrich) and 1:100 rabbit anti-CD31 (AB32457 ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
-
bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were lysed in RIPA buffer and processed according to the Western Blot protocol aforementioned with primary antibody against puromycin (Mouse, 1:1000, Sigma Aldrich #MABE343).
-
bioRxiv - Cell Biology 2023Quote: ... anti-tubulin antibodies (Serotec, MCA2047) were flowed into the chamber and the coverslip was blocked with 1% Pluronic F-127 (Sigma-Aldrich, CP2443) prior to introducing fluorescently labeled microtubules in Mg-BRB80 buffer supplemented with 7.5 μM taxol ...
-
bioRxiv - Cell Biology 2023Quote: ... and cells were incubated with 50μL primary antibody solution (dilution 1:1000) in 2% BSA (Bovine Serum Albumin, Sigma Cat. No.: A7906) at 4°C cold room overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Neuroscience 2023Quote: ... Tubulin was detected by incubating for 1 h at room temperature in 5% Milk-PBST (Marvel) with an α-tubulin antibody (Sigma T9026) at a 1:40.000 dilution.
-
bioRxiv - Molecular Biology 2023Quote: ... and FITC-labelled rabbit polyclonal antibody against wheat- germ-agglutinin (WGA) to visualize and quantify the myocyte cross-sectional area (1:100, Sigma Aldrich T4144). Cell surface areas and fibrotic areas were determined using ImageJ imaging software (http://rsb.info.nih.gov/ij/).
-
bioRxiv - Neuroscience 2023Quote: ... then incubated in 1xPBS + 0.5% Tritton X-100 + 3% Bovine Serum Albumin before adding the primary antibody (HPA036814, Sigma-Aldrich – 1/1000) for 48h at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
-
bioRxiv - Cell Biology 2023Quote: ... For immunolabeling of Cajal-Retzius like cells the following antibodies were used at a dilution of 1:200: Reelin (mouse, Sigma-Aldrich, MAB5366), and Mitochondria (mouse ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were incubated at RT for 1 h with fluorescently labelled secondary antibodies and Hoechst-33342 (Sigma-Aldrich, cat.no. B2261-25MG), which were diluted in blocking buffer as well.
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with bovine serum albumin (0.5%) before labeling with anti-STIM1 monoclonal antibody (1:50 dilution; 30 min; Sigma-Aldrich, Rockford, IL). Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were incubated with the corresponding secondary antibodies (see Table S2) for 1 h at room temperature and mounted in FluoroShield with DAPI (Sigma, F6057-20ml) for imaging ...
-
bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed in TBS-T and incubated overnight at 4 °C in the same buffer with anti-TH monoclonal antibodies (1:500; Millipore, Stockholm, Sweden). Immunoreactivity was detected using a secondary antibody conjugated with Alexa fluorescent dye (1:400 ...
-
bioRxiv - Microbiology 2024Quote: ... followed by a two-hour incubation at room temperature in darkness with FITC-coupled α-rabbit antibodies (diluted at 1:200 in BSA-PBS from Sigma Aldrich). After subsequent washing and drying ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in antibodies for 48h at 4 °C in a blocking solution and immunolabeled with chicken anti-Nefh (1:5000, EMD Millipore, AB5539), mouse anti-Syn1 (1:1000 ...
-
bioRxiv - Microbiology 2020Quote: ... and overlaid with standard cell culture media plus 0.37% (w/vol) sodium bicarbonate and 0.8% (w/vol) Aquacide II (Millipore Calbiochem). After 7 days incubation at 37 °C ...
-
bioRxiv - Systems Biology 2019Quote: ... Chk-2 inhibitor II BML-277 (used at 10 μM) from and Nutlin-3 (used at 0.75 - 4 μM, #N6287) from Sigma.
-
bioRxiv - Immunology 2019Quote: ... Data was obtained through the Amnis ImageStreamX Mark II imaging flow cytometer and INSPIRE Software (EMD Millipore, Billerica, MA). The accompanying IDEAS Software was used to perform data analysis ...
-
bioRxiv - Physiology 2019Quote: ... the cells were treated with various concentrations of cis-Diammineplatinum(II) dichloride (25, 40, or 60 μM; P4392, Sigma) diluted in 0.9% NaCl solution ...
-
bioRxiv - Microbiology 2019Quote: ... 5 µl of resuspended culture was added to 30 µl of RPMI-1640 media (without Albumax II) containing Hoechst 33342 (1µg/ml) (Sigma) and imaged at room temperature after 5min incubation at 37°C ...
-
bioRxiv - Physiology 2019Quote: ... mucosa were first washed in Hanks balanced salt solution (no Ca2+/Mg2+) to remove mucus before incubating in 2 ml dissociation media containing 2.4 U/ml Dispase II (Sigma) and 0.5% collagenase Type I (Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were maintained in appropriate volume of Dulbecco’s Modified Eagle Medium supplemented with 10% serum (Serum Plus - II, Sigma), 100 units/mL penicillin and 100 units/mL streptomycin (Corning ...
-
bioRxiv - Synthetic Biology 2021Quote: ... cells were treated with 20 µM cis-Diamineplatinum(II) dichloride (cisplatin, stock diluted in 0.9% NaCl, Millipore Sigma, #479306) for 48 hours.
-
bioRxiv - Neuroscience 2021Quote: ... All MDCK II cell lines in this study were cultured in Minimum Essential Medium Eagle (MEM) (Sigma, cat. #M4655) supplemented with 5% FBS ...
-
bioRxiv - Microbiology 2021Quote: ... Attached cells were imaged by brightfield microscopy and by CLSM after staining with 20x SYBR Green II (Sigma Aldrich).
-
bioRxiv - Cell Biology 2021Quote: ... Molecular sizes were confirmed using DIG-labeled DNA Molecular Weight Marker II (λDNA with Hind III digestion) (Sigma; #11218590910). Genotyping PCR and DNA sequencing analysis (Figure S1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail Set I #524624 and Phosphatase Inhibitor Cocktail Set II #524625; Millipore Sigma-Aldrich), was added directly on the plated cells and lysed for 15 min on ice ...
-
bioRxiv - Molecular Biology 2020Quote: ... The tissue was gently digested in a filter sterilized Dispase II solution (0.5 U/ml; Sigma Aldrich, Taufkirchen, Germany) in complete media composed of DMEM-F12 (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... Denuded fail to fertilize M-II oocytes (n=8) were obtained 36h post-ovulation and cultured briefly (2-4h) in 50µl KSOM medium (Millipore) containing 0.4% BSA at 37°C ...
-
bioRxiv - Pathology 2019Quote: ... progenitors were incubated in basal medium supplemented with Ang-II (10 nM) or TGF-β (10 nM) (Sigma-Aldrich) for 7 or 21 days ...
-
bioRxiv - Physiology 2023Quote: ... cold PBS and then placed in a 1.5 mL microcentrifuge tube containing: 200 μL 4.5 mg/mL Collagenase II (C6885, Sigma-Aldrich), 0.5 μL 10 mM Y-27632 (10005583 ...
-
bioRxiv - Physiology 2022Quote: ... cells were washed and replenished with 0.5% FBS containing RPMI medium which was supplemented with an iron solution 330 μM Fe(II)Sulfate (F8633, Sigma) and 330 μM Fe(III)Nitrate (F8508 ...
-
bioRxiv - Molecular Biology 2024Quote: ... the lungs were enzymatically digested in a buffer containing 2 mg/ml Dispase II (D4693, Sigma-Aldrich, Steinheim, Germany), 2 mg/ml Collagenase IV (17104019 ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by thoroughly chopping with scissors for 5 minutes and digesting with 15 ml of lysis buffer (PBS, 1.3 mM CaCl2, 2.4 unit/mL dispase II (Sigma D4693), and 1.5 unit/mL collagenase D ...
-
bioRxiv - Neuroscience 2023Quote: ... Each animal was administered manganese (II) chloride tetrahydrate (MnCl2; 66 mg/kg ip, Sigma Aldrich Co., St. Louis, MD) after attachment to the shaker arm ...
-
bioRxiv - Genetics 2024Quote: ... cells were transferred into SFEM II supplemented with 3 U/mL hEPO (SCT) and 3% AB human serum (Sigma) for an additional 5 days of culture ...
-
bioRxiv - Cancer Biology 2024Quote: ... 100 μl of 10x concentrated virus in Type 2 medium was added to the well (instead of unconditioned Type 2 medium) for 72 hours along with polybrene (Sigma, #H9268; final concentration = 8 µg/ml). After transduction with pDual_dsCas9_Venus ...