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Citations for Millipore Sigma :
8101 - 8150 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Human GBM commercial cell lines were maintained routinely in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, New York, NY, USA), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Microbiology 2024Quote: ... Chips were coated overnight at 4°C with ECM composed of 200 ug/mL of human Collagen IV (Sigma) + 100 ug/mL of Matrigel (Corning) ...
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bioRxiv - Cell Biology 2024Quote: ... 3,3’ s-dihexyloxacarbocyanine iodine (DiOC6) and fibrinogen from human plasma were obtained from Sigma Aldrich (St. Louis, Missouri, USA). ML161 (PAR1 inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3 times with PBST and 100 μL of o-phenylenediamine dihydrochloride (SigmaFast OPD, Sigma) substrate was added per well ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3 times with PBST and 100 µL of o-phenylenediamine dihydrochloride (SigmaFast OPD, Sigma) substrate was added per well ...
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bioRxiv - Biophysics 2021Quote: ... The cantilevers were functionalized with amine groups by immersing in 2% (vol/vol) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Cancer Biology 2021Quote: ... PreScission protease-eluted supernatant was added to 100 mL of S-protein agarose (EMD Millipore, 69704-3) to capture S-tagged protein ...
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bioRxiv - Biophysics 2021Quote: ... the glass slides were dissolved in the ethanol solution of 1% (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
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bioRxiv - Cell Biology 2019Quote: Total cellular RNA was labeled for 3 hours with EU (Thermo-Fisher, E10345) or BrU (Sigma, 850187). EU-RNA was detected using the Click-iT RNA Imaging Kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated muscles from female C57BL/6J mice were pre-incubated with 40 µM IPA-3 (Sigma-Aldrich) or a corresponding amount of DMSO (0.11% ...
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bioRxiv - Cell Biology 2020Quote: ... then 85°C for 10 minutes and transfected in a 3:1 polyethylenimine (PEI, Sigma Aldrich, 408747):DNA ratio into 70% confluent HEK 293A cells (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... then were stimulated with either 10 nM DHT (5α-Androstan-17β-ol-3-one, Dihydrotestosterone, A8380, Sigma) or EtOH (Vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were placed in a 3:1 solution of acetone to Durcupan ACM resin mixture (Sigma-Aldrich) for 2 hrs ...
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bioRxiv - Microbiology 2019Quote: Primers used in this study (Table 2) were designed with Primer 3 (http://bioinfo.ut.ee/primer3-0.4.0/) and synthesized by Sigma-Aldrich.
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bioRxiv - Developmental Biology 2021Quote: Zona pellucida were removed from embryos with 3-4 min pronase (0.5% w/v Proteinase K, Sigma P8811 ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were anesthetized with Ethyl 3-aminobenzoate methanesulfonate salt (MS-222, Tricaine, Sigma-Aldrich, St. Louis, MO) and adults were euthanized by rapid cooling as previously described (Wilson et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... After three washes (15 min) with PBS supplemented with 3 mg/ml poly-vinylpyrrolidone (PVP) (Sigma, P0930), embryos were permeabilized with PBS containing 5% DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich; Cat. No. A7906) in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) for 15 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed with 1X PBS and embedded in 3% agarose Type I-B (Sigma-Aldrich, St. Louis, MO) dissolved in 1X PBS ...
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bioRxiv - Genomics 2022Quote: ... both diluted in 1× TNB (1M Tris HCl pH 7.5, 3 M NaCl-blocking reagent, Sigma-Aldrich) with posterior incubation for 1 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 3 days at 4°C with rabbit anti-AVP (1:2,000, Sigma, PC234L) or rabbit anti-Ntng1 (1:1,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... a 500 μl 50% suspension of 3 times pre-washed streptavidin agarose beads (Novagen, Cat. N°69203) (i.e. ...
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bioRxiv - Molecular Biology 2021Quote: A CU RNA hairpin (5’-AAAAGAAAAGACGCGUAGUUUUCUACGCG- 3’) labeled with Cyanine 5.5 at the 5’-end (Millipore Sigma) was annealed in 20 mM HEPES ...
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bioRxiv - Genomics 2020Quote: ... RNA was degraded by the addition of 3 μL of 33 mg/mL RNase A (Sigma R4642) and incubation at 37°C for 2 hours ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were washed in PBS followed by staining with 2.5 uM Fluo-3 AM (Sigma) in FACS buffer (5% FBS in HBSS ...
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bioRxiv - Genetics 2020Quote: ... cells were UV-irradiated (10 J/m2) through isopore polycarbonate membranes containing 3-μm-diameter pores (Millipore) and experiments were performed 2hr post-UV irradiation ...
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bioRxiv - Biophysics 2019Quote: ... DSPC (1,2-1,2-distearoyl-sn-glycero-3-phosphocholine)) were purchased from Sigma Aldrich (St. Louis, MO, USA); lipid dry powders were dispersed in defined amounts of chloroform ...
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bioRxiv - Bioengineering 2019Quote: ... followed by addition of 1.12 g N-hydroxysuccinimide [9.72 mM] and 1.79 g of N-(3-(dimethylamino)propyl)-N’-ethylcarbodiimide hydrochloride [9.36 mM] (Sigma). The resulting solution was mixed at room temperature (RT °C ...
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bioRxiv - Physiology 2019Quote: ... GST-free eluates of protein were prepared by using 1 unit of biotinylated thrombin (Millipore 69672-3) per 100 μl of beads and letting samples incubate on a rotator at 4° C overnight ...
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bioRxiv - Biophysics 2019Quote: Cholesterol depletion was performed by treating the cells with 3 mM methyl-β-cyclodextrin (Sigma Aldrich, Germany). The cells were incubated for 30 minutes were then measured.
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bioRxiv - Neuroscience 2019Quote: ThioT stock solution (3 mM) was filtered through a 0.2-μm syringe filter (Sigma-Aldrich. Cat#T3516). The stock solution was diluted into the aggregation buffer (20 mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... Cells were washed with PBS 3 times and incubated with fluorophore-conjugated secondary antibody TRITC (Sigma Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... followed by blocking of endogenous peroxidase with 3% H2O2 (Sigma H-1009; Sigma-Aldrich Chemie GmbH, Germany) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were further washed in PBS containing 0.3% Triton and 3% bovine serum albumin (BSA; Sigma Aldrich) for 2 x 5 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2019Quote: Cell viability was assessed with 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, #M5655, Sigma Aldrich) after treatment with MMC and the PARP inhibitor Olaparib (AZD2281 ...
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bioRxiv - Biophysics 2019Quote: ... The ARL6:GTP complex was then loaded onto 3 ml of anti-Flag M2 affinity resin (Sigma). The resin was washed with 30 ml of buffer + GTP to remove any excess ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Physiology 2020Quote: Adi14-3-3ζKO mice were injected with 1 mg/kg CL-316,243 (Sigma-Aldrich, St. Louis, MO) following an overnight fast ...
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bioRxiv - Genomics 2021Quote: ... 100 µL of filtered must were incubated with 3 µL of DEEM (Sigma-Aldrich 87-13-8) for 30 min in a sonication bath at room temperature in a solution containing 580 µL of borate buffer (pH 9 ...
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bioRxiv - Genomics 2021Quote: ... decapsulated testes of 3-month old mice were treated with 1 mg/ml of collagenase (Millipore/Sigma) to digest interstitial tissue and release single tubules in suspension ...
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bioRxiv - Immunology 2021Quote: ... Optical density following development with 3-amino-9-ethylcarbazole (AEC) substrate (Sigma Aldrich, Saint Louis, MO, USA) was used to calculate the 50% micro neutralization titer (50% MN ...
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bioRxiv - Genomics 2021Quote: ... decapsulated testes of 3-month old mice were treated with 1 mg/ml of collagenase (Millipore/Sigma) to digest interstitial tissue and release single tubules in suspension ...
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bioRxiv - Neuroscience 2021Quote: ... or treated for 3 min with 30 µM N-Methyl-D-aspartic acid (NMDA) (Sigma-Aldrich, M3262). After 30 min slices were incubated for 10 min with AcTEV or vehicle control ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 centrifugal filters (3 kDa molecular weight cut-off, Millipore). Reducing agent (2 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... The abdominal cavity was opened and 3 mL of 1.87 mg/mL collagenase V (Cat# C9263, Sigma;) in Hanks’ Balanced Salt Solution (HBSS ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were infiltrated with a graded series of Hexamethyldisilizane (HMDS, CAS# 999-97-3, Sigma Aldrich) in ethanol (25% - 50% - 75% - 100% - 100% ...