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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Slices were subsequently washed 3 x 5 minutes in PBST 0.01% (DPBS ThermoFisher, Tween P1379 Sigma), permeabilised for 15 minutes in PBST 0.5% and washed in PBST 0.01% a further three times ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 60 µL NADPH (N7505, Sigma; 2 mg/3 mL assay buffer). The formation of TNB was monitored kinetically at 412 nm over 5 min in a spectrophotometer (Fluostar Omega ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was reduced with 3 μL of freshly prepared 1 M DTT (Sigma, #43815-5G) for 15 min at 60°C ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed (3 times, 5 min each) with PBS containing 0.1% Triton X100 (Sigma-Aldrich), and then incubated with secondary antibody (Table 1 ...
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bioRxiv - Physiology 2024Quote: qPCR-cells were separated into media (control) or nicotine ((Sigma Cat. # 6019-06-3, 50μg/ml) for 360min then lysed and RNA was isolated using the easy-spin Total RNA extraction kit (Boca scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 300 μM of [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine sulfonate] Ferrozine (Sigma-Aldrich, Bangalore, India) was added in -Fe media ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed in 3x 3 min changes of PBS with 0.1% Tween-20 (PBST) (Sigma P1379). Slides were blocked with secondary antibody serum for 1 hr at room temperature (RT) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.3% CHAPS) supplemented with Phosphatase Inhibitor Cocktail 2 and Cocktail 3 (Sigma-Aldrich, P5726 and P0044) and Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Biophysics 2021Quote: ... Micropatterned coverslips were functionalized with a solution of 10 µg mL-1 FN from human plasma (Sigma Aldrich #F1056) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... solutions of 89Zr-labeled TDM1 (4 μCi/μg) were prepared in PBS (pH 7.5) containing 1% w/v human serum albumin (HSA, Sigma) and 0.1% w/v sodium azide (NaN3 ...
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bioRxiv - Immunology 2021Quote: ... that serves as support for the culture system was prepared seeding human dermal fibroblasts (200,000) re-suspended in a fibrin gel made of fibrinogen (Merck-Millipore), thrombin (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... TIFs (Telomerase immortalised fibroblasts29) and human embryonic kidney (HEK)293Ts cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (D5796, Sigma). All cell culture medium was supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 1×105 ESCs were plated on a 3.8 cm2 plate coated with human plasma fibronectin (Millipore, cat. no. FC010) and cultured in N2B27 medium supplemented with 12 ng/ml bFGF (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... and 5 μm sections were prepared and incubated with a mouse anti-human IgG4 mAb (clone MRQ-44; Sigma) or isotype control ...
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bioRxiv - Synthetic Biology 2019Quote: ... we grew the cells in plastic 48-well plates that were pretreated with 50 μg/mL human fibronectin (Millipore) for at least 10 min at 37 °C before cell plating (to improve cell adherence) ...
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bioRxiv - Cancer Biology 2019Quote: The AsPC-1 and Capan-2 human PC cell lines were obtained from Sigma-Aldrich (St. Louis, MO, USA) and Thermo Fisher (Waltham ...
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bioRxiv - Neuroscience 2019Quote: ... the eye cup was treated for ∼ 15 minutes with human plasmin (∼ 50 µg mL−1, Sigma or Haematologic Technologies) to aid vitreous removal.
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bioRxiv - Biochemistry 2020Quote: ... Other antibodies used in this study include those specific to human CD3 (UCHT1 clone, Cat. number 217570, Merk-Millipore), Vav1 (Cat ...
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bioRxiv - Microbiology 2019Quote: ... a human telomerase immortalised retinal pigment epithelial line was passaged in Dulbecco’s modified minimal essential medium (DMEM)-F12 (Sigma) supplemented with 200 mM glutamine ...
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bioRxiv - Immunology 2019Quote: ... monocytes were cultured at 1 × 106 cells/ml in RPMI-1640 medium supplemented with 1% human AB serum (Sigma), 20 ng/ml M-CSF (Peprotech) ...
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bioRxiv - Molecular Biology 2020Quote: ... Human HEK-293T cells were grown in Dulbecco’s Modified Eagle’s high glucose medium with HEPES (Sigma-Aldrich®, D6171) supplemented with 10% fetal bovine serum ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... an additional blocking step was performed by incubating cells with 2 mg/ml γ-globulins from human blood (Sigma) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were washed with 60x slurry volume in lysis buffer and eluted through overnight incubation at 4°C with 160μl of Human Rhinovirus (HRV)-3C protease (Millipore) in a final volume of 2-3ml ...
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bioRxiv - Bioengineering 2020Quote: ... Three sections at varying depths within the tumor are immunostained with mouse anti-human nuclei (clone 235-1, Millipore) followed by secondary Dylight 488 horse anti-mouse (Vector) ...
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bioRxiv - Synthetic Biology 2021Quote: ... IPSCs were plated at 25,000 cells/cm2 in a 24-well plate coated with 15µg/ml human laminin (Sigma, USA). The following day ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded onto glass coverslips coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation) at a density of 80,000 cells and 40,000 cells for the PDGFRα homodimer cell line and PDGFRβ homodimer cell line ...
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bioRxiv - Cancer Biology 2020Quote: Human AGCT-derived KGN cells (Riken, Tsukuba, Japan) and JGCT-derived COV434 cells (Sigma-Aldrich, St. Louis, MO, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)-Ham’s F12 (Caisson ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human neuroblastoma cells (SH-SY5Y) were obtained from the European Collection of Cell Cultures (ECACC, Sigma-Aldrich, Dorset, UK) and grown in a 1:1 minimal essential medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... The activated fluidic channels were coated with 0.5 mg/mL collagen type IV human placenta (Sigma Aldrich, C5533-5MG) in the cell culture incubator overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature ...
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bioRxiv - Genetics 2021Quote: Human embryonic kidney (Hek) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Biochemistry 2020Quote: ... adherent human embryonic kidney (HEK) 293T cells were transfected with the expression plasmids with branched polyehtyleneimine (PEI, Sigma-Aldrich). In detail ...
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bioRxiv - Cell Biology 2021Quote: ... Human cells were trypsinized and incubated in cold PBS supplemented with 5% fetal calf serum (Sigma-Aldrich; PBS-FACS). DNA was stained either by propidium iodide (PI) ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30 μg/mL fibronectin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and the cleavage fragments of C3b were detected by immunoblotting with a polyclonal anti-human C3b antibody (HPA020432; Sigma) (1:1000) ...
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bioRxiv - Cancer Biology 2020Quote: ... The human glioma initiating cells (GS7-11, GS8-11, GSC280) were maintained in serum-free DMEM/F12 medium (Sigma), containing B27 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Black Falcon clear flat bottom 96-well plates were coated with 5 μg/mL human collagen I (Sigma-Aldrich) in 2 mM acetic acid (Merck) ...
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bioRxiv - Cancer Biology 2020Quote: Glioma cells were grown as attached on cell-treated vessels coated with 10ug/mL human plasma fibronectin (Millipore, FC010) and transfected with X2 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 6μl of 10μM siRNAs targeting human KLC1 or KLC2 were mixed with 12μl HiPerFect in high-glucose DMEM medium (Sigma-Aldrich) and combined with 1×105 cells per well of a 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... and MDA-MB-231 (triple-negative human breast adenocarcinoma) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium with HEPES modification; Sigma, D1152) supplemented with 10 % fetal bovine serum (FCS ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney epithelium 293T (HEK293T) cells and Hela cells were cultured in DMEM-high glucose (Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at a density of 4×104 to 5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12-well plate in GK15 medium (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBS was replaced with a solution of TCNB buffer (50mM Tris, pH7.5, with 100mM NaCl, 10mM CaCl2) containing 89.5 nM human MMP9 (Sigma SAE0078) and incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Microbiology 2021Quote: ... THP-1 (ATCC® TIB-202™) human monocyte cell line was cultured in RPMI-1640 medium (Sigma, R8758) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human adenocarcinoma alveolar basal epithelial cells (A549, ATCC) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: Human colorectal adenocarcinoma cell lines HT29 and HCT116 (both European Collection of Cell Cultures through Sigma-Aldrich, Dorset, UK) were grown in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biochemistry 2021Quote: The hnRNP L_RRM2 fragment (residues189-286) was amplified from the human brain cDNA library and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (termed p28a) ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...