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Citations for Millipore Sigma :
8351 - 8400 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... The aE11-Fab sample was concentrated by centrifugal concentrator with a MWCO of 3 kDa (Merck Millipore) and further purified by size exclusion chromatography into 20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was pre-coated with fibronectin (3 μg/ml, Sigma). The microfluidic device was generated according to our previous report31 ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were concentrated to a final concentration of ∼3 mg/ml by using Centricon filters (Millipore), aliquoted and flash-frozen in liquid nitrogen for −80°C storage ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction containing Pcf1_ED or Pcf1_ED* were concentrated using Amicon centrifuge filter units of 3 kDa cutoff (Millipore) flash freezed in liquid nitrogen and stored at −20 °C or −70 °C.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with a solution of 3% (w/v) BSA (Sigma-Aldrich, St. Louis, MO, USA) and 0.1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... DLD1 cells were treated for 2 h with 0.5 mM 3-indoleacetic acid (Sigma, stock in ethanol). At the time points indicated ...
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bioRxiv - Cell Biology 2024Quote: ... medroxyprogesterone acetate (Selleck S2567) and N6,2′-O-dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP) (Sigma D0627) for 6 days.
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bioRxiv - Biophysics 2024Quote: ... Membranes were then incubated for 3 min with Amersham ECL TM Western Blotting detection reagents (Sigma, GERPN2106) or for 5 min with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Neuroscience 2024Quote: ... after 2-3 months of receiving HFD the mice were given 75 mg/kg STZ (Sigma-Aldrich) in 0.1M sodium citrate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Incubation with the 4-MU substrate (3 mM in citrate phosphate buffer with 0.2% taurodeoxycholate, Sigma-Aldrich) for 90 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... the surface was incubated for 10 min with biotinylated BSA (Sigma-Aldrich, 3 mg/ml in PBS), washed several times with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... in PBS for 10 min at room temperature and blocked with 3% bovine serum albumin (A9647, Sigma), 2% donkey serum (ab7475 ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were quenched with 3x 3 mins washes of quench solution containing 20 mM Sodium Ascorbate (Sigma), 10 mM Trolox (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-fenylindol (DAPI; 1:10 000; Sigma-Aldrich, Saint-Louis, MO, USA, 28718-90-3) and phosphate buffer (PB ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-fenylindol (DAPI; 1:10 000; Sigma-Aldrich, Saint-Louis, MO, USA, 28718-90-3) and phosphate buffer (PB ...
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bioRxiv - Microbiology 2024Quote: ... 53.36 nmoles of the purified Hcp1 (18.74 kDa) was mixed with 5,336.00 nmoles of (3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma) and 5,336.00 nmoles N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2024Quote: ... ESCs were cultured in N2B27 medium with 2i (PD0325901, 0.4 μM: WAKO, CHIR99021, 3 μM: Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... and then incubated for an additional 3 h with phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) PMA (25 nM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pyrophosphatase solution [NEB] (0.1Unit/ml) and polyethylene glycol (PEG-8000; Sigma-Aldrich, Cat. No. 25322-68-3) into the mixture to a final concentration of 5% (w/v ...
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bioRxiv - Genetics 2024Quote: ... tardigrades were maintained in 1.2% agar plates overlaid with sterilized pure water (Elix Advantage 3 UV, Millipore) containing live chlorella suspension (Recentec ...
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bioRxiv - Cell Biology 2024Quote: ... then re-suspended into 3 mL of spheroplast buffer containing 50 mM β-mercaptoethanol (#63689, Sigma-Aldrich) + Zymolyase (#ZE1005 ...
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bioRxiv - Immunology 2024Quote: ... silastic tubes were filled with ∼2.5 mg DHT (5α-Androstan-17β-ol-3-one, ≥97.5%, Sigma-Aldrich) and trimmed to a total length of 5 mm ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were concentrated to a final concentration of ∼3 mg/ml by using Centricon filters (Millipore), aliquoted and flash-frozen in liquid nitrogen for −80°C storage ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T and Calu-3 (ATCC-HTB-55) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma), supplemented with 10% FBS (VWR) ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma), and other primary antibodies were used at a 1:1000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed 3 times with PBS and permeabilized with 0.1% Triton X-100 (Sigma, 15 min). Cells were incubated with primary and secondary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cdx2CreERAPCfl/fl mice were injected intraperitoneally at day 0 and day 3 with 0.8 mg tamoxifen (Sigma) from a stock solution at 4 mg/ml dissolved in corn oil (Sigma) ...
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bioRxiv - Genetics 2023Quote: ... we subjected the amplified PCR products to electrophoresis using gels consisting of 2-3% agarose (Sigma Aldrich) in 1 x Tris-acetate-EDTA (1 x TAE ...
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bioRxiv - Genomics 2023Quote: ... Concentrated cells (∼500 µL) were transferred dropwise to 3 mL ice-cold TRI reagent (Sigma-Aldrich T9424) while vortexing ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 3 times with PBS-T (Sigma cat. # P3563-10PAK) and blocked for 1h with 1% BSA (for the mouse sera ...
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bioRxiv - Microbiology 2023Quote: ... mice (n=5 mice for each line) were orally administered with IAA (3-indoleacetic acid, auxin, Sigma) and by an intraperitoneal injection on a daily basis (Brown and Sibley ...
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bioRxiv - Bioengineering 2023Quote: ... putida CAP (pSEVA438_MBPeGFP) culture induced with 1 mM of 3-MB (Sigma-Aldrich, St Louis, MO, USA) were extracted from a bioreactor at OD600 of 2.25 ...
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bioRxiv - Systems Biology 2023Quote: ... containing the internal standard sodium 3 (trimethylsilyl)propionate-2,2,3,3,-d4 (TSP, 98 atom % D, Sigma-Aldrich, Milan) at a 0.21 mM final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was resuspended in 3 mL of 3.5% Collagenase/Dispase (Sigma, USA, 11097113001, 100mg/mL stock) in Hibernate A minus Calcium Media (BrainBits ...
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bioRxiv - Bioengineering 2022Quote: A 10 mg/mL solution of monosodium iodoacetate (MIA; Sigma Aldrich, Burlington, MA; CAS: 305-53-3) was prepared in sterile saline ...
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bioRxiv - Synthetic Biology 2023Quote: ... pH 7 using repetitive washing and centrifugation with an Amicon 3 kDa MWCO centrifugal filter (Merck Millipore). For the synthesis of ditopic A’-A’ CC ligand ...
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bioRxiv - Pathology 2023Quote: ... Lorea (500 seeds) were soaked in 3 mL.g-1 of seeds of phosphate-buffered saline (PBS, Sigma) plus Tween 20 (0.02% v/v ...
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bioRxiv - Neuroscience 2023Quote: ... length = 8.08 + 0.26 cm) were anesthetized with MS-222 (ethyl 3-amino benzoate methanesulfonic acid, Sigma Aldrich) and were ovariectomized by removing ovaries and fat bodies using a cauterizer through a small incision (~1 cm ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody was diluted in a solution of 3% bovine serum albumin (BSA; Cat#A7906, Sigma-Aldrich), 2% donkey serum (DS ...
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bioRxiv - Bioengineering 2023Quote: ... A second solution was prepared with 80 milligrams of 3-(4-hydroxyphenyl) propanoic acid (HPA; Sigma Aldrich), 80 milligrams EDC ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was then passed through a 3 kDa molecular weight cutoff filter (Amicon Ultra, Sigma-Aldrich) placed in a collection tube by centrifuging the sample tube at 15,000g for 45 min at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... The coverslips were treated with 1% v/v solution of (3-acryloxypropyl)trimethoxysilane (APTS, Merck - Sigma Aldrich) in ethanol for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed with DPBS x 3 at room temperature and then stained with DAPI (Sigma Aldrich). Tissues were washed with DPBS and then mounted on charged glass slides ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Samples then were permeabilized and blocked with 0.1% Triton-X in PBS containing 3% BSA (Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with DPBS and incubated with BE1 supplemented with 3 µM Chiron (CHIR99021, Sigma-Aldrich) and 100 ng/mL activin A ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was composed of DMEM/F-12 (3:1 vol/vol; D5921, N4888, Sigma-Aldrich) containing 0.3% FCS ...
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bioRxiv - Genomics 2023Quote: ... The supernatants were transferred to fresh tubes and 100 µL of BCP (1-bromo-3-chloropropane, Sigma) were added ...
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bioRxiv - Cancer Biology 2023Quote: ... The sample was stained with antibody against pH3 (phosphorylated histone 3, 1:800, Sigma-Aldrich 06-570) and H2 (Hemese ...