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Citations for Millipore Sigma :
8051 - 8100 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Primary human hepatocytes were treated with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, 200 ng/ml, Sigma, #T9701) for 6 hours to induce apoptosis ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Pathology 2024Quote: ... This mixture was then pipetted into Mantarray casting wells containing 50µL EHT culture media with 0.3U human thrombin (Sigma-Aldrich). EHTs were then incubated for one hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were cultured in human O+ erythrocytes (deidentified from commercial vendor) at 4% hematocrit (HCT) in RPMI-1640 (Sigma) supplemented with 25 mM HEPES (4-(2-hydroxyethel)-1-piperazineethanesulfonic acid ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Cell Biology 2024Quote: ... The probe was resuspended in a solution containing 38 ng/μL human Cot-1 DNA (Millipore Sigma, Cat # 11581074001), 256 ng/μL yeast tRNA (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and AP-labeled goat anti-human IgA (α-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) was used for detection ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (HCT116, human colon cancer cell line, ATCC) were transduced with lentivirus using 8 μg/ml polybrene (Sigma). 72 hours after lentivirus transduction ...
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bioRxiv - Biochemistry 2022Quote: ... residues 353-598) and human RXRα (NR2B1) LBD (residues 223-462) that were inserted into a pET45b(+) plasmid (Novagen) as a TEV-cleavable N-terminal hexahistidine(6xHis)-tag fusion protein ...
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bioRxiv - Cancer Biology 2023Quote: Two individual pre-designed human PLXNB2 sgRNA CRISPR-Sanger clones and one non-targeting control vector (Sigma-Aldrich #0020) were ordered from Sigma as glycerol stock (Sigma-Aldrich #HS5000013567 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were extensively washed in 0.1M PB and TBS and blocked in 1% human serum albumin (HSA; Sigma-Aldrich) solution for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48h later by intraperitoneal injection of 5 IU human chorionic gonadotropin (PMS from SIGMA, HGC from Millipore USA). Mouse zygotes were obtained by mating B6/CBA stud males with superovulated B6/CBA females at a 1:1 ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48h later by intraperitoneal injection of 5 IU human chorionic gonadotropin (PMS from SIGMA, HGC from Millipore USA). Mouse zygotes were obtained by mating B6/CBA stud males with superovulated B6/CBA females at a 1:1 ratio ...
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bioRxiv - Molecular Biology 2023Quote: Human induced pluripotent stem cell derived cardiac fibroblasts (iPSC-FBs) were cultured in FibroGRO Complete Medium (SCMF001; Millipore Sigma) with 2% FBS at 37°C in a humidified incubator with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphate buffered saline (PBS) containing 0.12% of DMSO and 4% of human serum albumin (HSA) (Sigma-Aldrich, MO, USA) was used as the negative control and incubation buffer ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼30% CD90+ stromal cells were dissociated at 15 days using human cardiac digestion buffer: 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation using Hisopaque-1077 (Millipore) and SepMate PBMC isolation tubes (StemCell ...
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bioRxiv - Microbiology 2023Quote: ... Human liver hepatocellular carcinoma (HepG2) and Vero (African Green Monkey Kidney) cells (ATCC) were regularly maintained in DMEM (Sigma) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... we added a calibration range by serially diluting a polyclonal IgG antibody from human sera solution (reference: I2511, Sigma), to reach concentrations ranging from 0.68 ng.mL-1 to 22 ng.mL-1 ...
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bioRxiv - Immunology 2023Quote: Human Vγ9Vδ2 T cells were specifically expanded from fresh PBMCs ex vivo using 5 µM zoledronic acid (Sigma Aldrich) or 3 µM BrHPP (bromohydrin pyrophosphate ...
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bioRxiv - Cell Biology 2023Quote: ... human breast cancer (MCF7) and human embryonic kidney cells (HEK293T) cells were cultured in Dulbeco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Microbiology 2023Quote: ... The degradation products of fibrinogen were detected by Western blot using rabbit anti-human fibrinogen polyclonal antibodies (Sigma-Aldrich) at a 1:5,000 dilution as described above.
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bioRxiv - Pathology 2023Quote: Human or mouse lung tissue slides (7 μm) were fixed in 3.7% formalin (Sigma-Aldrich, St. Louis, MA, USA), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... All-trans Retinoic Acid (RA) (R2625) and Brain-derived neurotrophic factor (BDNF human) (B3795) were purchased from Sigma Aldrich.
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bioRxiv - Cell Biology 2023Quote: Human mini-colons cultured in biological triplicates were extracted from the PDMS chips and digested with collagenase I (Sigma) at 100 U/ml concentration in Advanced DMEM/F12 with penicillin/streptomycin ...
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bioRxiv - Immunology 2023Quote: Lentiviral vectors containing validated shRNA sequences targeting expression of human PRKCD (accession NM_006254) or a non-target control were obtained from Sigma (catalog numbers TRCN0000010193 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were seeded onto plasticware coated with a solution of 0.1 % gelatin containing 10 µg/ml human plasma fibronectin (FN) (Millipore). and collagen type 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human peripheral blood mononuclear cells (PBMCs) (FUJIFILM Wako Pure Chemical, Osaka, Japan) were cultured in RPMI1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell were then fixed as previously described and all three batches were permeabilised with Triton X-100 0.5% in PBS and a Cy5-conjugated anti-human (AC112S, Sigma) was used for the detection of T-DM1 and T-DXd ...
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bioRxiv - Genomics 2023Quote: The human pre-B ALL cell line Reh (DSMZ, ACC 22) was maintained in RPMI-1640 medium (Sigma, R0883) supplemented with 2 mM L-Glutamine (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Human plasma like media (HPLM) was generated according to the published formulation59 with addition of 5% dialyzed FBS (Sigma) and Pen/Strep (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: Chromosomes were labeled by either injecting mRNA encoding human H2B-mCherry or H1 protein purified from calf thymus (Sigma) labeled with AlexaFluor 568 or 647 as described previously (Bun et al. ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2023Quote: Human cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D6429) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Membrane attack complex (MAC) binding was assessed by staining with rabbit anti-human C5b-C9 antibody (Catalog #204903, Sigma) at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903 ...
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bioRxiv - Microbiology 2024Quote: Human neutrophils from the peripheral blood of healthy blood donors were isolated by density gradient centrifugation (Histopaque; Sigma–Aldrich) followed by hypotonic lysis of erythrocytes ...
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bioRxiv - Cell Biology 2024Quote: Madin-Darby canine kidney cells with stable expression of human α-2,6-sialyltransferase (MDCK-SIAT1) (Sigma, catalog #: 05071502-1VL), human lung adenocarcinoma epithelial A549-Dual cells (InvivoGen ...
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bioRxiv - Bioengineering 2024Quote: ... and glucagon were measured using a customized MILLIPLEX human metabolic Hormone Panel V3 (Millipore Sigma, cat. no. HMH3-34K). CCL2 ...
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bioRxiv - Cell Biology 2024Quote: ... and human breast cancer cells (4175-LuT; Hoshino et al., 2015) were cultured in Dulbecco’s modified Eagle’s medium (Sigma‒ Aldrich) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) and blocked in 1% human serum albumin (Sigma-Aldrich) in TBS ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... human embryonic kidney HEK-293 T cells and human dermal fibroblasts (HDFs) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington, MA, USA, cat# SCC507) and cultured in vendor-specified homebrew media ...