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Citations for Millipore Sigma :
8051 - 8100 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were washed with PBS and fixed with 4% paraformaldehyde at room temperature for 15 minutes and washed with PBS before being labelled with anti-phospho-Histone H2A.X (Ser139) antibody (Sigma Aldrich, Cat#:05-636) 1:500 or anti-Thymine dimer antibody (Novus Biological ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells producing HCMV specific antigens were detected 24h post infection by immunoperoxidase staining using monoclonal antibodies directed against the UL123-coded 72 kDa immediate early antigen 1 (IEA1) (Mouse Anti CMV IEA, MAB8131, Millipore, Temecula, CA, USA) and 120h post-infection by immunoperoxidase staining using monoclonal antibodies directed against UL55-encoded late antigen gB (LA ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated with the primary antibody overnight at 4 C (Anti-Histone γ-H2AX, #07-627 clone PC130, Millipore Sigma 1:1000) and the secondary antibody (Goat anti-Mouse Alexa Fluor 647 Polyclonal ...
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bioRxiv - Zoology 2019Quote: ... then washed in PBS with 0,1% Triton X100 and incubated in the secondary anti-rabbit CF488 antibody (SAB4600044, Sigma-Aldrich, diluted 1:1000) for 8 hours ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 0.1% DMSO in 1x PBS) and placed overnight (16h) at 4°C in 30 µL anti-phospho-histone H3 (Ser10) primary antibody (Millipore Sigma, Burlington, MA) diluted 1:100 in blocking solution ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:100; 06-570), in combination with the cardiomyocyte marker Anti-Troponin I Antibody (cTnI, 1:1000; MAB1691) (both Millipore Sigma, Burlington, MA). Fluorophore-conjugated secondary antibodies (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated overnight with primary antibodies (rabbit phospho-AURKA Thr288, 1:50; mouse anti-γ-tubulin GTU-88, Sigma-Aldrich; 1:1000) diluted in blocking buffer at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... with the addition of the cross-linking of the anti-flag M2 antibody to the Dynabeads using dimethyl pimelimidate (DMP) (Sigma-Aldrich, D-8388). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... solution in PBS for 4min and blocked with 1% BSA (Fisher) in PBS for 30min and stained with the anti-pY primary antibody (4G10 Platinum, EMD Millipore, 1:1000) in PBS 0.2% BSA for 1h ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Microbiology 2021Quote: ... and PilA2-Flag was detected using a horse radish peroxidase (HRP)-coupled primary anti-Flag antibody at a 1:2000 dilution (Sigma, cat. no. SAB4200119). Enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2021Quote: ... followed by washing with TBST (4x 5 min) and incubation with a goat anti-mouse alkaline phosphatase secondary antibody (Sigma-Aldrich, Steinheim, Germany) diluted 1:30 000 in TBST containing 3 % (w/v ...
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bioRxiv - Biophysics 2021Quote: ... Cat #TIB-180) were subjected to UV-crosslinking and U2AF2–RNA complexes were immunoprecipitated with 8 μg anti-U2AF2 antibody (Sigma-Aldrich, Cat #U4758) and Dynabeads Protein G (ThermoFisher Sci. ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in PBS containing 0.02% Triton X-100 and 2% NDS overnight at 4°C with primary antibodies: anti-ChAT (1:500, goat polyclonal, Millipore Cat# AB144P, RRID:AB_2079751) and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244 ...
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Microbiology 2020Quote: ... Cellular β-actin was used as a loading control and detected using a mouse anti-β-actin antibody (catalog no. A1987; Sigma-Aldrich). Horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibodies were used to detect the primary antibodies (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... non-fat milk in TBST buffer (Tris-buffered saline containing 0.05% Tween-20) overnight at 4 °C and incubated with anti-FLAG antibody (Sigma, F1804, 1:1000 dilution) or anti-HA antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... and probed over-night at 4°C either in TBS-TM with a 1:1000 anti-Flag antibody (Sigma-Aldrich; catalogue No F3165), or with a 1:10000 anti-LpoA antibody (loading control 28 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were added for 30 min to 1 h at the following dilutions: mouse anti-polyubiquitin FK1 (1:250; EMD Millipore, 04-262), guinea pig anti-p62 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... The blot was washed with TBST (4x 5 min) and incubated with a goat anti-rabbit alkaline phosphatase secondary antibody (Sigma-Aldrich, Steinheim, Germany) diluted 1:30,000 in TBST containing 3 % (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated overnight at 4°C with the following primary antibodies: mouse anti-DCP2 (Sigma, Saint Louis, MI, USA; 1:20), rabbit anti-LSm4 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... blots were incubated overnight at 4 °C with the corresponding primary antibody: anti-phospho-histone 2AX (Ser139) (EMD Millipore, 05-636, 1:1000), anti-phospho-Akt (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Neuroscience 2021Quote: ... subjected to heat induced antigen retrieval in a citrate buffer and incubated with a mouse anti-NeuN antibody (clone A60, Millipore, MAB377, 1:100). The used secondary antibodies were mouse linker antibody (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-β-actin antibody (AC-15; catalog #A5441) utilized for western blot (1:10,000) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Alexa 568-conjugated secondary antibody (catalog# A11031 ...
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bioRxiv - Cell Biology 2020Quote: ... The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore, 17-10086). Chromatin DNAs quality was assessed by 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the following primary antibodies for one hour at room temperature: mouse anti-α-tubulin (DM1α) 1:500 (Millipore Sigma #T6199), rat anti-tubulin clone YL1/2 1:1500 (Millipore Sigma #MAB1864) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for primary culture neurons (supplier, catalog number and working dilution are indicated): anti-Gad67 ms IgG2a (Millipore MAB5406, 1:500); anti-somatostatin rat (Millipore MAB354 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the incubation of cells with horseradish peroxidase (HRP)-coupled anti-FLAG M2 antibody (dilution-1:5000; Sigma-Aldrich; cat. no. A8592), prepared in 1% BSA for 90min ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Sections of the brainstem that contained CTB labeled neurons or mCherry neurons were incubated in a solution containing a mouse anti-TH monoclonal antibody (1:10,000; T-2928, Sigma-Aldrich, Oakville, ON, Canada) for 2 days ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, Cat No A2179) in PBST ...
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bioRxiv - Neuroscience 2019Quote: ... spinal motor neurons from six sections covering a distance of 640 µm were immunolabelled using a goat anti-choline acetyltransferase (ChAT) antibody (Merk Millipore, AB144P, 1/50) and a biotinylated donkey anti-goat IgG (Jackson ...
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bioRxiv - Neuroscience 2019Quote: ... Sections incubated for 24 hours at 4°C in a primary antibody solution containing mouse anti-NeuN (catalogue no. MAB377; Millipore, Billerica, MA, USA) diluted 1:250 in 0.3% Triton-X and 3% normal donkey serum (NDS ...
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bioRxiv - Immunology 2021Quote: ... Samples were then incubated in secondary antibody conjugates Atto 647N (1 μg/mL each of anti-mouse; Sigma catalog no. 50185-1ML-F) for 60min ...
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bioRxiv - Microbiology 2021Quote: ... cells were treated for approximately 18h with 5 μM 5-Azacytidine to covalently trap Mt2 or AMt2 with its target cellular RNA before RNA immunoprecipitation using an anti-FLAG antibody following manufacturer’s protocols (SAB4301135 - Sigma-Aldrich, 1:100 dilution) (23).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with PBS to remove residual puromycin followed by staining of cells using Alexa fluor-488 anti-puromycin antibody (Millipore, Cat#MABE343-AF488).
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bioRxiv - Neuroscience 2022Quote: ... sections were blocked for 1 hour in 10% normal goat serum (at room temperature) and probed with an anti-NeuN Alexa Fluor 488 conjugated antibody (1:100; Millipore-Sigma cat. # MAB377X) for 2 hours at room temperature and protected from light ...
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bioRxiv - Genetics 2022Quote: ... For normalization of signals, we used actin or Pgk1, which was detected by using mouse anti-actin monoclonal antibody (1:5000, (Millipore Cat# MAB1501, RRID:AB_2223041)) or mouse anti-Pgk1 antibody (1:10000 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Cultured human cortical neurons of 100-120 days post-differentiation were immunostained as above with primary antibodies against MAP2 (Mouse-anti-MAP2, Sigma, 1:200 dilution), VGLUT1 (Mouse-anti-VGLUT ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Genomics 2019Quote: ... the membrane was incubated with properly diluted primary antibody (M2 Monoclonal ANTI-FLAG 1:1000, F1804, Sigma; Monoclonal GAPDH 1:5000, 2118S, CST) in 5% dried milk/TBST overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed three times in 1x TBST and then incubated for 1 hour at 22°C in anti-mouse IgG HRP conjugate secondary antibody (Sigma, 2834819; 1:1000). Membranes were washed three times in 1x TBST before adding enhanced chemiluminescence detection reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... blocked in 3% BSA followed by overnight staining with lectin GS-II-Alexa594 and an anti-αSMA-alexa488 antibody (1:150, Sigma, in-house conjugated) in 0.5% triton in PBS ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates from different groups were collected and incubated with magnetic bead RIP buffer containing anti-human argonaute 2 (Ago2) antibody (Millipore, Billerica, MA, USA) and anti-human CPEB2 antibody (Proteintech ...
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...