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Citations for Millipore Sigma :
7901 - 7950 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... mid-sagittal cerebellar vibratome sections were blocked and permeabilized with 10% normal goat serum in PBS containing 0.2% Triton X-100 and immunostained with a mouse anti-Calbindin D28k antibody (Sigma-Aldrich, 1:1000) followed by incubation with Alexa Fluor 555-labeled goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog #11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution: anti-smooth muscle actin (SMA) 1:500 (Sigma, A2547), anti-GFP 1:200 (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... Triton-X 100 in 1x PBS) for 10 min after which it was incubated with primary antibody solution (anti-alpha-tubulin, #T5168, Sigma-Aldrich, Germany) in IF buffer diluted 1:500 for 1.5 h at room temperature while shaking followed by washing thrice with 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated overnight at 4°C with mouse anti-TH antibody (1:8000 dilution, T2928, Sigma-Aldrich, St. Louise, MO, USA), 1% NHS ...
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bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and developed by detecting bound IgG or IgA with appropriate fluorochrome-labelled secondary antibodies: 1 µg/ml goat anti-human IgG-Cy3 (Sigma, Cat# C2571) or goat anti-human IgA-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... The membranes that were loaded with fetal membrane and uterine tissue lysates were re-probed for 1 h at room temperature with a mouse anti-β-actin (ACTB) monoclonal antibody (Cat# A5441, Sigma-Aldrich). Then ...
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 20 000 rpm for 30 min at 4 °C and supernatants were analyzed by Western Blot using monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody (Sigma-Aldrich, A8592).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed again and incubated for 1 hour min with secondary FITC-conjugated goat anti-rabbit antibody (Sigma-Aldrich; 1:100) and TRITC-conjugated phalloidin ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells on coverslips were blocked with blocking solution (2% BSA/PBST) for 30 min and incubated with the primary antibodies anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were diluted in 10% goat serum and incubated for 18 hours at 4°C with laminin rabbit anti-mouse antibody (1:250, Sigma-Aldrich, L9393). Sections were then incubated for 1 hour at room temperature in their corresponding goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... the treated cells were performed using anti-chicken IgY (IgG) (whole molecule)-FITC antibody produced in rabbit (Sigma–Aldrich, dilution 1:640) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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Characterization of pediatric porcine pulmonary valves as a model for tissue engineered heart valvesbioRxiv - Bioengineering 2023Quote: ... The co-stain dye was prepared from a 1:50 dilution of anti-α-SMA CY3 conjugated primary antibody (Sigma, cat #C6198) and 1:50 dilution of anti-vimentin Alexa Fluor™ 488 conjugated primary antibody (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 µl wash buffer containing 10 µl 1 µg/µl anti-rabbit secondary antibody raised in guinea pig (Sigma-Aldrich, SAB3700889) and 1 U/µl RNase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... The grid was blotted with filter paper and floated on a 20 µl drop of primary anti-HA antibody (Sigma-Aldrich #H6908) diluted 1:500 in blocking buffer for 1 h in a closed ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins of the Co-IP were analyzed by Western Blot with the Monoclonal ANTI-FALG® M2 antibody produced in mouse (Sigma Aldrich), proteins of inputs were analyzed by Western Blot with the Anti-GFP from mouse (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 16-h incubation in PBS with 0.1% Triton™ X-100 with the following antibodies: mouse monoclonal anti-NeuN (1:500; EMD Millipore, MAB377), rabbit polyclonal anti-KCC2 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... 600 μl aliquots of diluted chromatin were incubated with 7 μl of the rabbit anti-trimethyl-Histone H3 (Lys27) antibody (Millipore, 07-449) in a rotator at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then washed and stained with 1:100 dilution of mouse anti-sarcomeric α-actinin primary antibody (A7811, Sigma-Aldrich) and 1:100 rabbit anti-CD31 (AB32457 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with 5% BSA in 0.1% TBS-Tween for 1 hr at room temperature for blocking step and incubated with 1:1000 anti-RFC1 antibody produced in rabbit (Sigma-Aldrich; AV44167) at +4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Microtubules were linked to PEG-silanized coverslips by incubating chambers with 33 μg/mL monoclonal anti-beta-tubulin III (neuronal) antibody (Sigma-Aldrich, T8578) for 5 minutes prior to washing with 10 mg/mL BSA for 2 minutes ...
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bioRxiv - Immunology 2023Quote: ... To ensure efficient and reproducible protein immobilization a preliminary array validation was carried out with anti-FLAG antibodies (Sigma-Aldrich, cat# F7425) 1:5000 and mouse anti-His6 tag polyclonal antibodies (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... with 0.2% triton X-100 and for 12 h at 4 °C in a humidified chamber with the primary antibody: mouse-anti-NeuN (Neuronal Nuclei, Sigma-Aldrich MAB377) or goat-anti-MMP-9 (Matrix metallopeptidase 9 ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Bioengineering 2023Quote: ... Blockade of collagen VI-integrin interactions was performed using monoclonal antibodies against integrin β1 (10 μg/mL anti-integrin β1, Millipore sigma, MAB2253), Cilengitide (5 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were then transferred from the 96-well plates into opaque white 384-well plates that were pre-coated with monoclonal anti-FLAG M2 antibody (Millipore Sigma, F1804). Plates were blocked with 1% BSA/5% sucrose/0.5% Tween 20/1xPBS ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C in blocking solution with the addition of the following antibodies (dependent on experiment): goat anti-ChAT (Millipore Sigma AB144P), rabbit anti-cFos (Synaptic System 226 008) ...