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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were probed with the following primary antibodies at room temperature for 1-2 hours or at 4 ℃ overnight: anti-GAPDH (Millipore, #MAB374, 1:5,000 dilution), anti-Flag M2 (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Goat anti-rabbit (Alexa fluorTM 488 #A-11034) was added against the primary antibody and mounted with DAPI medium (#F6057, Sigma, St. Louis, MI, USA). Fluorescence was captured in an inverted microscope (Nikon Eclipse TE2000-U).
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by SDS-PAGE and Western analysis was performed using either mouse anti-FLAG antibody (1:1000; Sigma-Aldrich, St. Louis, MO); rabbit anti-GFP (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed 10 min three times in PBS 1X-Tween buffer and then incubated for one h at room temperature with peroxydase labeled anti-rabbit donkey antibody (#NIF 824 ECL Rabbit IgG, HRP-linked whole Ab, Sigma-Aldrich Life Science, USA) diluted in saturation buffer at 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST) buffer with 5% bovine serum albumin (BSA) and incubated with primary rabbit anti-β-actin antibody (Millipore Sigma, cat. #SAB5600204) at 1:20000 ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with primary antibody to stain tubulin (mouse anti-α-tubulin B-5-1-2 mAb, Sigma-Aldrich, T5168, dilution 1:250) for 4 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: The following primary and secondary antibodies were used at indicated concentrations: anti-human α-actinin1 (mouse monoclonal, BM75.2, Sigma Aldrich, A5044; WB 1:1000), anti-human talin (mouse monoclonal ...
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bioRxiv - Genetics 2022Quote: ... 2% normal goat or donkey serum and 0.1% Triton X-100 in PBS (-) at room temperature for 1 h and reacted with mouse anti-parvalbumin monoclonal antibody (Sigma-Aldrich, P3088, 1:4000), goat anti-somatostatin antibody (Santa-Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... The second and third series were processed for Calbindin (using mouse monoclonal anti-Calbindin-D-28K antibodies, 1:1000 dilution; Millipore Sigma, St. Louis, MO) and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... the organs were incubated in goat anti-Shh primary antibody (Table 1) in 10% normal donkey serum (NDS; D9663, Sigma Aldrich, St Louis, MO) in PBS/MT at 4°C for 48 hr ...
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bioRxiv - Immunology 2022Quote: ... and then incubated for 1 hour at 37°C with secondary goat anti-rabbit antibodies conjugated with fluorescein isothiocyanate (FITC; 1:250; Sigma-Aldrich, Prague, Czech Republic). Fluorescence intensity was measured using the Synergy H1 microplate reader (BioTek ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then washed multiple times with PBSTx before being incubated in FITC-conjugated goat anti-mouse secondary antibody (Sigma cat. #A6667, 1:200) for 2-5 hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 x 106 – 1 x 107 cell equivalents were analyzed on PAA / SDS gels and probed with anti-HA antibodies (Sigma, rat 3F10, 1:1000), anti-Myc antibodies (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... wing discs treated as described above and eye/antennal discs were fixed and subjected to IF using a mouse anti-FLAG antibody (Sigma-Aldrich, F3165, 1:100) and anti-mouse-Alexa568 (Molecular Probes ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Cell Biology 2023Quote: ... blocked with 5 % non-fat dry milk in TBST (TBS with 0.1 % Tween-20) and probed at 4 °C overnight with the following primary antibodies: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich F3165, 1:1000), mouse monoclonal anti-α-tubulin B512 (Sigma-Aldrich T5168 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by immediate fixation with 4% paraformaldehyde in PBS at 37⁰C and then immunostaining with anti-puromycin antibody (Sigma Aldrich, clone 12D10, MABE343).
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bioRxiv - Neuroscience 2024Quote: ... while the second and third series were processed for Calbindin (using mouse monoclonal anti-Calbindin-D-28K antibodies, 1:1000 dilution; Millipore Sigma, St. Louis, MO) and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... After overnight incubation the primary antibodies were washed away and PLA secondary probes were added: Anti-Rabbit MINUS (Sigma Aldrich, #DUO92005, dilution 1:5), Anti-Goat PLUS (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated with slight agitation (40 rotations/min) overnight at 4°C with a rat anti-muscarinic acetylcholine receptor M2 (M2 AChR) primary antibody (1:500, Sigma-Aldrich, reference number MAB367) in the same blocking solution ...
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bioRxiv - Molecular Biology 2024Quote: ... Guts were incubated overnight at 4°C with a primary anti-catalase antibody (C0979, Sigma; 1:250 in 5% w/v BSA in PBS). After three washes with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... reducing RAD2l levels about 40% relative to our CTCF-EGFP line (using Simple Western of asynchronous cell lysates, RAD2l detected via anti-RAD2l antibody (0S-908, Merck Millipore, l:S0, Suppl. Fig. 4G). 0verexpression of RAD2l rescued this effect ...
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bioRxiv - Microbiology 2024Quote: ... Fixed cells were washed twice with PBS + 0.1% Tween 20 and incubated with 1:1000 mouse anti-HCMV IE-1 monoclonal antibody (Millipore, Catalog No: MAB810R, clone 8B1.2) in staining buffer (Dulbecco’s PBS [DPBS] containing 1% FBS and 0.3% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... Pro-inflammatory stress was induced by a cytokine mix composed of IL-1β (5 ng/mL, cat. I5271-Sigma Aldrich, St. Louis, MO, USA), TNFα (30 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... and then lysed for 15 min with a M-PER Mammalian protein extraction reagent (Pierce Biotechnology, Rockford, IL) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and 1 mM p-amidinophenyl methanesulfonylfluoride (PMSF) ...
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bioRxiv - Neuroscience 2024Quote: ... injections of 0.9% sterile saline (Nicolas Carrillo, Baxter Healthcare Corporation, Deerfield, IL, USA) or of cocaine (15mg/kg, Sigma-Aldrich, St Louis, MO, USA). During days 6-12 animals remained in their home cages undisturbed ...
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bioRxiv - Immunology 2024Quote: ... and culture supernatants were harvested after 24 h for analysis of cytokines (CXCL1 and IL-6) and MMP2 activity (Luminex bead array, Millipore Sigma, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... Subsequent cytokine measurements interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were done using Milliplex magnetic bead panel (Millipore Sigma, MA, US) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cryopreserved blood plasma was thawed at room temperature and used for IL17a detection by a commercial mouse IL-17a ELISA Kit (Sigma Aldrich catalog #RAB0263-1KT). The test was performed according to manufacturer guidelines ...
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bioRxiv - Physiology 2024Quote: ... Pups were initially fed with 50 μL of Esbilac formula (PetAg, Hampshire, IL) with or without dextran sodium sulfate (DSS, Sigma-Aldrich, St Louis, MO) supplementation ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with primary antibodies (pp71 antibody kindly provided by Thomas Shenk, dilution-1:100 in 0.1% BSA; Daxx antibody (MABE1911, Sigma-Aldrich, St. Louis, MO), dilution-1:50 in 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% NaN3) and incubated with primary antibody (monoclonal α-V5-Tag mouse antibody /Elabscience/ and monoclonal α-Flag M2 mouse antibody /Sigma Aldrich/) and secondary antibody (AF488-conjugated goat anti-mouse antibody /Elabscience/) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody was detected using an HRP-conjugated secondary antibody raised in rabbit (Sigma). Chemoluminescent detection was achieved using an ECL Prime Western Blot detection kit (GE HealthCare) ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies were either alkaline phosphatase-coupled IgG antibodies (1:10.000) from Sigma-Aldrich or horseradish peroxidase-coupled IgG antibodies (1:8000 ...
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bioRxiv - Molecular Biology 2020Quote: ... or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745, clone MC315; Merck Millipore). DNA was purified by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Immunology 2022Quote: ... Cells were subsequently incubated with primary antibody and visualized with Alexa secondary antibody (Sigma).
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bioRxiv - Plant Biology 2019Quote: ... Bound antibodies were labeled with a peroxidase-conjugated goat antibody to mouse immunoglobulins (Sigma), and peroxidase activities were then determined ...
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bioRxiv - Microbiology 2020Quote: ... transferred to PVDF membranes and probed with α-FLAG antibody (Sigma, M2 monoclonal antibody) and detected with chemiluminescence.
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (PSD95 1:500, Antibodies Inc., Davis CA and MAP2 1:1000; Millipore) were diluted in 1x PBS with 1% BSA and 0.2% Tween ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit polyclonal calbindin antibody (C8666) and mouse monoclonal NeuN antibody (clone A60; Millipore, MAB377B) were from Sigma-Aldrich (Taufkirchen ...
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bioRxiv - Physiology 2019Quote: ... Pax7 primary antibody (1:100, DSHB) and laminin primary antibody (1:100, Sigma-Aldrich) were diluted in 1% TSA blocking buffer and applied overnight ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used: monoclonal antibodies against β-actin and GFP (Sigma-Aldrich), Gos28 and GM130 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... β-Actin mouse antibody (#A2228) and actin rabbit antibody (#A2066) were purchased from Sigma-Aldrich. Anti-Active Caspase-3 antibody (#559565 ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... resuspended in 250 μl antibody binding buffer and 2.5 μg FLAG M2 antibody (Sigma) per 25 μl of Protein A/G magnetic beads and incubated overnight at 4°C with rotation ...
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bioRxiv - Plant Biology 2020Quote: ... MPK6 antibody (Sigma-Aldrich; A7104) or Phospho-p44/42 MAPK (erk1/2 ...
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bioRxiv - Plant Biology 2020Quote: ... MPK3 antibody (Sigma-Aldrich; M8318), MPK6 antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibody 1H6 (Millipore, # MABE1126) was diluted in 0.5% goat serum/PBST and incubate with coverslips at 37°C for 1 hr or at 4°C overnight ...