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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed in PBS and PBS-TX followed by incubation with the relevant primary antibody (rabbit anti choleratoxin (Sigma-Aldrich UK) or rabbit anti-Mcherry (1:2000 dilution ...
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bioRxiv - Developmental Biology 2019Quote: ... the membranes were washed 3x in TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2019Quote: Immunoprecipitations were set up with 100 µl solubilised chromatin (roughly equivalent to 4 × 106 cells) and 3 µg of an anti-acetylated histone H3 antibody (Millipore, 06-599) in a total of 1 ml Native ChIP Incubation Buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Molecular Biology 2021Quote: The following antibodies were used for Western blotting at the indicated dilution: anti-FLAG-HRP conjugate (Sigma M2 A8592-1MG, 1:5000); anti-MBTD1 (Abcam ab116361 ...
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bioRxiv - Pathology 2021Quote: ... Rabbit polyclonal anti-calnexin (CNX) antibody (Cat# SAB4503258, RRID:AB_10746486) and all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Genetics 2020Quote: ... 3xFLAG-EED expression and deletion of eed deletion were confirmed by western blots probed with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) and genotyped by PCR with primers ...
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bioRxiv - Systems Biology 2020Quote: ... Resolved proteins were transferred to nitrocellulose membranes and detected by standard Western blotting using monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and HRP labelled anti-mouse IgG secondary antibody (Cell Signalling technology ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were permeabilized and non-specific binding sites were blocked before incubation with rabbit anti-TH antibody (1:1000, AB152, Millipore Sigma, USA) for 48h ...
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining.
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining ...
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bioRxiv - Biophysics 2019Quote: FLAG-BLM (40 nM) was conjugated to Quantum Dots (QDs) by first pre-incubating a biotinylated anti-FLAG antibody (Sigma-Aldrich, F9291) with streptavidin QDs (Life Tech. ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-calbindin (1:1000, mouse, Sigma Aldrich, #C9848). After 3 rinses with PBS for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in a phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled in 2x sample buffer for 5 min to elute the proteins and analyzed by SDS-PAGE followed by Western blot using anti c-myc antibody (Sigma-Aldrich, #C3956).
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bioRxiv - Microbiology 2021Quote: ... (80) and α-PilC (1:5000 dilution) (81) antibodies were used together with horseradish-conjugated goat anti-rabbit immunoglobulin G (Sigma-Aldrich) or anti-mouse sheep IgG antibody (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight followed by goat anti-rabbit secondary antibody conjugated with Cy3 for 1 hr at room temperature (1:500, Millipore, Cat. #AP132C). Since the purpose of this staining was to evaluate the presence of extracellular HMGB1 in the tendon ...
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bioRxiv - Cell Biology 2021Quote: ... tissue sections were washed 3 times with PBS and incubated at room temperature for 2 hrs with Cy3-conjugated goat anti-rabbit IgG antibody (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... For KIF18A localization analyses the following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (MAB1864; Sigma Aldrich), rabbit anti-KIF18A 1:100 (A301-080A ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Genetics 2019Quote: ... 0.1%SDS and 1% Tween 20 and re-stained for the loading using the anti-glucose-6-phosphate dehydrogenase antibody (Millipore, cat #A9521). Blots were again imaged on ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog # 11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) or 5ug Normal Mouse IgG Polyclonal Antibody (MERCK ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were analysed using 4-12% Bis-Tris polyacrylamide gels in MOPS buffer followed by Coomassie staining or western blot using the following antibodies: Mouse anti-FLAG M2 (Sigma, 1:10,000). RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... ovaries were incubated overnight at 4°C under agitation with the primary antibody (anti-pTyr (4G10) (05-321, Millipore Sigma, 1:40), anti-pERM (3141S ...
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bioRxiv - Developmental Biology 2022Quote: ... and cultures were incubated overnight at 4°C with primary antibodies diluted in TBS/10% goat serum: mouse anti-β-tubulin-III (Sigma #T8660) or mouse anti-MAP2 (Sigma ...
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Each strain to be tested was then washed twice with sterile 1x PBS and adjusted to a concentration of ~2×105 cells/mL in macrophage growth media containing 1 μg/mL of the anti-GXM antibody 18B7 (Sigma Cat# MABF2069). Cells were opsonized in this media for 1h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Sixty μl of Dynabeads Protein G were incubated for 1h30 at 4°C under agitation with 16 μg of the anti-Ago2 antibody (Sigma-Aldrich, SAB4200085) in 1X PBS containing 0.1% of Tween 20 ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were then rinsed 3 times in 0.3% triton X-100/PBS and incubated for one hour at room temperature with the secondary fluorescent antibody (Donkey, anti-goat, Alexa fluor 488 secondary, Sigma-Aldrich - USA) concentration (1:1000) ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore, MAB1574, 1:5,000).
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bioRxiv - Neuroscience 2022Quote: ... Larvae were washed three times in 0.1% PBST and incubated with secondary antibodies: anti-mouse Alexa Flour 647, 1:100 (Invitrogen, #28181. Stained larvae were mounted using DAPI Fluoroshield (Sigma-Aldrich, #F6182). Images were collected on a Nikon A1 eclipse Ti confocal microscope.
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bioRxiv - Molecular Biology 2023Quote: ... ChIP experiments were performed basically in accordance with the above procedure using 1 µg of an anti-H4K16ac antibody (Millipore, 07-329) and 2.4 µg of an anti-H2A.Z antibody (Kudo et al ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated for 1 hour at room temperature with a goat anti-mouse alkaline phosphatase-conjugated secondary antibody (dilution 1: 5000; Sigma-Aldrich, USA) and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skim milk in TBST and target proteins were detected with ANTI-FLAG® M2 antibody (Sigma-Aldrich) and anti-α-tubulin (SantaCruz).
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bioRxiv - Neuroscience 2022Quote: ... immunohistochemical examination of all surgical brain specimens was performed using the following panel of antibodies: mouse monoclonal anti-NeuN (clone A-60, Millipore Cat#MAB377), mouse monoclonal anti-GFAP (clone 6F2 ...