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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The fractions were then pooled based on protein concentration and Amicon centrifugal filters (EMD Millipore) were used to concentrate the samples ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was eluted with a cocktail of 100 ug/ml RNase A (Sigma-Aldrich) and 0.1 units/ µL RNase H (Epicenter) ...
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bioRxiv - Cancer Biology 2023Quote: ... All the proteins were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore Corporation, UFC901024).
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bioRxiv - Neuroscience 2023Quote: ... We used primary antibodies for astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541). To quantify relative intensity of staining for GFAP we measured the average signal intensity in the region of interest and normalized it to that of the WT littermate mouse ...
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bioRxiv - Biochemistry 2023Quote: ... Polyubiquitinated proteins were visualized by immunoblotting for ubiquitin (Millipore Sigma 05-994, clone P4D1-A11) and actin was used as loading control (Millipore Sigma MAB1501R ...
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bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were detected by Western blotting using the following primary antibodies against: Flag (M2, Sigma), ERK1/2 (3A7 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were detected with ECL Prime Western blotting Amersham (Sigma Aldrich, cat. no GERPN2232) detection reagent and images acquired with STELLA imaging system ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Biophysics 2024Quote: ... the protein was further purified by binding to ANTI-FLAG M2 Magnetic Beads (Sigma Aldrich) and eluted with 3× FLAG Peptide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mg of nuclear protein was concentrated in 500ul using a Microcon centrifugal filter (Millipore) and then applied to a Superose 6 size exclusion column (10/300 GL GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblotting loading samples were prepared after adjusting the protein concentration using 5X SDS (Sigma-Aldrich) and denatured by boiling ...
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bioRxiv - Cancer Biology 2023Quote: Equal amounts of protein or EVs were mixed with reduced (containing 5% β-mercaptoethanol, Sigma) or non-reduced 4 x Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The AGO1 protein was detected by Western blot using a Flag-HRP antibody (A8592, Sigma).
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bioRxiv - Cell Biology 2023Quote: Protein lysates were resolved in SDS-PAGE gels and transferred to PVDF (Millipore, 0.2 µm) membrane ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then added with 75 μl pre-washed Protein A Sepharose (Sigma, #P6649) and incubated at 4 °C for 30 min with rotation ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following proteins were purchased from the indicated vendors: MAP2 (EMD Millipore AB5622); SLC17A6 (Alomone Labs AGC-036-GP) ...
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bioRxiv - Microbiology 2024Quote: ... protein expression was induced by adding 50 μM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma) and 1 mg/mL riboflavin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: Coding sequences for M and Enn proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site ...
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bioRxiv - Physiology 2024Quote: ... The separated proteins were transferred to an Immobilon PVDF-FL membrane (#IPFL00005, Millipore, Billerica, MA) at 100 V ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich), and stored in 20 mM HEPES ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein-containing fractions were concentrated using a 3 kDa cutoff Amicon centrifugal filter (Sigma UFC8003) and loaded onto a Superdex 75 size exclusion column equilibrated with a storage buffer (25 mM Na2HPO4 pH 7.0 ...
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bioRxiv - Pathology 2024Quote: ... Separated proteins were transferred onto polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA). The membranes were reacted with the following primary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were transferred onto a 0.45 μm pore polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore), previously activated in cold 100% methanol ...
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bioRxiv - Genetics 2024Quote: ... and the protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore, Merck).
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bioRxiv - Genetics 2024Quote: ... proteins were loaded on an 8% polyacrylamide gel and transferred onto a PVDF membrane (Millipore). Chemiluminescence was induced by Luminata Forte Western HRP Substrate (Millipore) ...
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bioRxiv - Developmental Biology 2024Quote: Proteins were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, IPVH00010) for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins solubilized into serum-containing media were pulled down by using heparin-sepharose beads (Sigma). Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5) ...
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bioRxiv - Molecular Biology 2024Quote: Protein samples were separated on Bis-Tris gels and transferred to a PVDF membrane (Millipore). For immunoblots showing multiple proteins with similar molecular weight ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to probe target proteins with the BCIP®/NBT Liquid Substrate (Sigma-Aldrich). ImageJ software (National Institutes of Health ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were concentrated to 1 mL with a 30K MWCO concentrator (EMD Millipore) and diluted with 1 mL buffer A ...
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bioRxiv - Microbiology 2024Quote: ... Eluted protein was concentrated to 2 mL by centrifugation using Amicon Ultra centrifugal filters (Millipore) with a 50-kDa molecular weight cut-off (MWCO ...
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bioRxiv - Plant Biology 2024Quote: Yeast protein was extracted by homogenizing yeast cells with acid-washed glass beads (Sigma-Aldrich) in homogenizing medium (0.25 M sorbitol ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Pathology 2024Quote: ... Proteins were then transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins on the gel were then transferred to Immobilon-FL PVDF membranes (Millipore) at 100V for 1 hr 12 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysates were mixed with 30 µL of Protein G Sepharose 4B (Sigma-Aldrich) and rotated at 4°C for 2 h with 2.5 µg of mouse anti-Myc antibody (Calbiochem) ...
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bioRxiv - Biochemistry 2024Quote: ... The disulfide bridges in the protein extract were reduced in 10 mM dithiothreitol (DTT, Sigma) for 1 h at 37 °C and shaking at 1000 rpm in a TC ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions which contained full-length protein were pooled and concentrated in an Amicon (Millipore) 4 mL centrifugal concentrator 30K MWCO to 50-100 µM ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions which contained full-length protein were pooled and concentrated in an Amicon (Millipore) 4 mL centrifugal concentrator 3K MWCO to 100-200 µM ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were digested overnight at 37°C with mass spectrometry grade trypsin (SOLu-Trypsin, Sigma) in an enzyme:substrate ratio of 1:50 ...
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bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra-15 Ultracell-30K centrifugal filter units (Millipore–Sigma), then centrifuged at 100,000 rpm at 4 °C for 10min using a Beckman TLA-120.2 rotor to remove aggregates ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra-15 Ultracell-30K centrifugal filter units (Millipore–Sigma), then centrifuged at 100,000 rpm at 4 °C for 10min using a Beckman TLA-120.2 rotor to remove aggregates ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated with addition of Trichloroacetic acid (TCA) 100% (1/5v/v) (Sigma #T6399) and washed with 0.01M HCl/ 90% Acetone before by peptide identification by LC/MS.
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bioRxiv - Systems Biology 2024Quote: ... and 0.25 ml 10 X endonuclease-free BugBuster Protein Extraction Reagent (Millipore, Cat. No. 70921) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Bait-purified prey proteins from plasma were then eluted with 5 mM biotin (Sigma-Aldrich) in ice-cold 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich 68957) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich 22790) ...