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Citations for Millipore Sigma :
8201 - 8250 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... USA) or the fluorescent signal of each protein was measured with a chemiluminescence detection system (Millipore) using Scion Image software (Scion Corp. ...
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bioRxiv - Plant Biology 2020Quote: ... Bound effectors and associated plant proteins were competitively eluted by 3XFLAG peptides (Sigma-Aldrich, Dorsett, England) and separated by SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... the mixed liposome and protein samples were analysed in Lumox®24-well plate (Millipore, USA) by live-cell imaging on Axio Examiner.Z1 (Zeiss microscope).
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bioRxiv - Systems Biology 2021Quote: ... the proteins the samples were reduced with 5 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 20 minutes in 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total protein was then incubated with anti-Flag antibody (M2 monoclonal clone, Sigma) for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich).
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bioRxiv - Immunology 2021Quote: ... Sheared chromatin was “pre-cleared” using Protein A Agarose/Salmon Sperm DNA beads from Sigma-Aldrich. Pre-cleared chromatin from the equivalent of 3.57×106 cells was used in the chromatin IP reactions along 3μg of either anti-TCF1 (C63D9 ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were immunoprecipitated by the addition of 3 µl of rabbit anti-FGFR3 (Sigma-Aldrich, F0425)/500 µg protein with protein A-agarose (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... proteins were fractionated on SDS-polyacrylamide gel electrophoresis gel and transferred to a nitrocellulose membrane (Millipore). Membranes were incubated with primary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Neuroscience 2019Quote: ... Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore, Bedford, MA, USA). After blocking with 5% (w/v ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were visualized on Microchemi 4.2 (DNR Bio Imaging Systems) using Immobilion Western ECL reagents (Millipore).
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bioRxiv - Immunology 2021Quote: ... The cells were harvested by centrifugation and lysed by a BugBuster Protein Extraction Reagent (Novagen, USA). Nanobodies were purified by IMAC using an AKTA start system and a HisTrap column ...
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bioRxiv - Microbiology 2020Quote: ... anti-PP1b that cross-reacts with both PP1b isoform and PP1c isoform proteins (Millipore, 07-1217), anti-phospho-PP1a (Thr320 ...
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bioRxiv - Cell Biology 2019Quote: ... isolated PMNs were preincubated with 20μM TMB-8 (Ca2+ antagonist and protein kinase C inhibitor; Sigma), for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was filtered through a 0.45 µm low protein-binding membrane (Millipore Steriflip HV/PVDF). To achieve a 300 times concentration of the GeCKO pooled library ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved by standard SDS-PAGE gels and transfer to Immobilon PVDF membranes (Sigma, #IPVH00010) and incubated with specific antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate protein concentration was determined using the Direct Detect Spectrometer (Millipore Sigma, Cat. No. #DDHW00010-WW) and normalized to 5 μg ...
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bioRxiv - Biophysics 2021Quote: Liposomes were separated from free protein on a Sepharose® CL-4B column (Sigma Aldrich #CL4B200). The conditions resulted in on average <<1 ctSTIM1 dimer per liposome.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on an SDS-PAGE gel and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked for at least 30 min in blocking solution (5% milk in 1X TBS-T ...
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bioRxiv - Neuroscience 2022Quote: RNA immunoprecipitation was performed using the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The specific protein bands were visualized with immobilon western chemiluminescent HRP substrate (Millipore, Sao Paulo, Brazil), captured by Alliance 4.7 (Uvitec ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using an Amicon ultra-15 centrifugal filter unit concentrator (Millipore, Burlington, MA) and loaded onto an ion-exchange column (HiTrap SP FF ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein expression was visualized using Immobilon ECL Ultra Western HRP Substrate (Millipore Sigma, catalog number WBULS0500) in combination with the BioRad ChemiDoc Touch Imaging system ...
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bioRxiv - Cancer Biology 2022Quote: ... Non-specific protein interactions were blocked by incubating with 10% goat serum (Sigma-Aldrich Cat# S26) for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The column was calibrated with standard proteins of known Stokes radii (Sigma-Aldrich, St. Louis, MO). Abl2 or Abl2-557-C and tubulin were mixed with tubulin at a ratio of 1:4 and incubated for 30 min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Pathology 2022Quote: ... The signal intensity of targeted protein was detected using ECL western blot detection solution (Millipore, WBKLS0500).
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bioRxiv - Molecular Biology 2022Quote: ... The precleared lysate was then transferred to Protein G Dynabeads coupled with anti-FLAG antibody (Sigma) and the mixture was incubated for 2 hours at 5°C on a roller ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The presence of transferred proteins on the membrane was confirmed by staining with Ponceau S (Sigma). Membranes were blocked for 2 hours at room temperature with 5% non-fat milk in 0.1% tween-20/TBS (TBS-T) ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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bioRxiv - Genomics 2022Quote: In vitro translated protein was purified over 20 µL of anti-HA agarose beads (Sigma-Aldrich), glutathione agarose beads (GoldBio) ...
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bioRxiv - Biophysics 2022Quote: ... Five microliters of a 5 mg mL−1 solution of protein G (Sigma-Aldrich, Tokyo, Japan) was added to the flow chamber (about 5 μL) ...
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bioRxiv - Immunology 2022Quote: ... coupled to protein A Sepharose magnetic beads (Ademtech 0423) and anti-TAX1BP1 antibody HPA024432 (Sigma-Aldrich) together with protein A Sepharose (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were harvested from the cells by subcellular fractionation using ProteoExtract subcellular fractionation kit (Millipore Sigma). Fractionated samples were analyzed by SDS-PAGE and electrophoresis for detection of HA-tagged Nedd4 (Roche anti-HA high affinity,1:2000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were fractionated on a 10% SDS-PA gel and transferred to PVDF membranes (Millipore, IPVH00010), which were then treated with 5% non-fat milk in 1× TBS buffer (50 mM Tris- HCl [pH 7.5] and 150 mM NaCl ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Diluted chromatin was pre-cleared with Salmon Sperm DNA/Protein A agarose slurry (Millipore, 16-157) at 50 μl slurry/ml of diluted chromatin in an end-to-end rotator overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were proceeded to SDS-PAGE and analyzed by silver staining (Sigma-Aldrich, PROTSIL2-1KT).
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bioRxiv - Microbiology 2021Quote: ... Protein was separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, Millipore). Blots were blocked using 5% milk in Tris-buffered saline containing 0.1% Tween 20 (TBS-T) ...