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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP) (EMD Millipore; AB5804), rabbit anti-microtubule associated protein-2 (MAP2 ...
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bioRxiv - Plant Biology 2022Quote: Protein samples were run on SDS-PAGE and transfer onto PVDF membrane (Immobilon-P, Millipore). The membrane was blocked by PVDF blocking reagent (Toyobo ...
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bioRxiv - Microbiology 2023Quote: ... Co-immunoprecipitated proteins were eluted for 1h at 4°C with 3X FLAG peptide (Sigma) in TBS (pH 7.4) ...
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bioRxiv - Physiology 2022Quote: ... the University of Michigan Transgenic Core injected Cas9 protein (Sigma-Aldrich, St. Louis, MO, USA), editing templates containing LoxP sites (IDT Ltd. ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... The ECL Western blotting Analysis System was used to reveal protein bands (Millipore, United States). Densitometry was performed with ImageJ software and standardized against β-actin.
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation of FLAG fusion proteins was performed using an anti-FLAG M2 affinity gel (Sigma) by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein levels were determined by immunoblotting using anti-FLAG clone M2 (Sigma, F3165, 1:3000).
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were transferred into fresh tubes and proteins concentrations were assessed using Bradford assay (Sigma). Supernatant was used as input fractions ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were transferred into fresh tubes and proteins concentrations were assessed using Bradford assay (Sigma). Sample were then mixed with 4x SDS buffer and boiled for 95°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μg protein per sample was alkylated for 30 min with 20 mM Iodoacetamide (Sigma) and digested with 5% trypsin (w/w ...
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bioRxiv - Plant Biology 2024Quote: ... The DDB2-FLAG fusion protein was detected using the anti-FLAG HRP (Sigma-Aldrich: A8592) at a 1:5,000 (v ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized by chemiluminescence using either Immobilon Chemiluminescent HRP substrate ECL (Millipore #WBKLS0500) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher #34095) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated using Amicon 10K and 30K columns (Millipore, Ref. UFC803096; UFC801096) until the final protein concentration reached approximately 1 to 2 mg/mL ...
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Physiology 2024Quote: ... viral media was collected and passed through a 0.45 μM low-protein binding filter (Millipore) and concentrated by low-speed centrifugation (3300 g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins bands were immobilized through transfer to PVDF membrane (IPFL00010, Immobilon Transfer Membrane, Millipore, Ireland) for 90 min at 30 volts in ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were diluted to the same protein concentration using 2X Laemmli buffer (Sigma-Aldrich), boiled for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to 0.45 µm pore size polyvinylidene difluoride membranes (PVDF) (Millipore, catalog # IPVH00010), incubated with rabbit anti-LC3B antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The final protein was concentrated by using Amicon Ultra 30 kDa MWCO filters (Millipore Sigma).
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bioRxiv - Microbiology 2024Quote: ... the protein was concentrated to approximately 30 mg/ml using an Amicon Ultracel-10K (Millipore) and then subjected to size-exclusion chromatography using either an S75/26–60 column or an S200/26–60 (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following proteins were purchased from the indicated vendors: MAP2 (EMD Millipore AB5622); SLC17A6 (Alomone Labs AGC-036-GP) ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were detected and visualized for protein bands with Immobilon Forte western HRP substrate (Millipore) and imaged in Image Quant LAS4000 ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mg lysate proteins were mixed with 200 µL of anti-Flag M2 (Sigma-Aldrich) or anti-HA (Thermo Scientific ...
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bioRxiv - Genetics 2024Quote: ... Proteins were transferred to either NitroBind nitrocellulose (GE, EP4HY00010) or Immobilon-FL PVDF (Millipore, IPFL00010) membranes in Tris-Glycine transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... 5-100 ng of S-RBD protein (SinoBiological, 40592-V08B) or ovalbumin (Sigma-Aldrich, A5503) recombinant protein were added as control ...
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bioRxiv - Immunology 2024Quote: ... and RIP assays were carried out with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore) and anti-Flag antibody (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Luminol Reagent (Millipore, WBKLS0500) and ChemiDoc XRS+ Imaging system (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Biophysics 2024Quote: ... then protein was eluted with elution buffer (wash buffer with 10 mM d-Desthiobiotin (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblotting loading samples were prepared after adjusting the protein concentration using 5X SDS (Sigma-Aldrich) and denatured by boiling ...
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bioRxiv - Biophysics 2024Quote: ... the protein was further purified by binding to ANTI-FLAG M2 Magnetic Beads (Sigma Aldrich) and eluted with 3× FLAG Peptide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mg of nuclear protein was concentrated in 500ul using a Microcon centrifugal filter (Millipore) and then applied to a Superose 6 size exclusion column (10/300 GL GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, I6758) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Systems Biology 2022Quote: Single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was estimated using 4% copper sulfate and bicinchoninic acid (Sigma, USA, Cat#B9643). Protein samples were separated by 8-12% SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The amount of total protein in the samples was quantified using the Bradford assay (Sigma). An equal concentration of protein samples was mixed with 2X Laemmli buffer (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: ... After electrophoresis proteins were transferred to a Polyvinylidene fluoride (PVDF; Immobilon-P™, Millipore®) membrane using semi-dry blotting (Amersham biosciences TE 77 ECL Semi-Dry Transfer Unit ...
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bioRxiv - Physiology 2023Quote: ... STIM-1 polyclonal (Protein Tech group) was used at 1:250 and HCN4 polyclonal (Sigma) was used at 1:500 ...