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Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Immunostaining was performed as previously described [84] using the following antibodies: Mouse anti-Rac1 (Millipore Cat#05-389-25UG, 1:200), Rabbit anti-RhoA (Proteintech Cat#10749-1-AP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were incubated overnight in a humidified chamber at room temperature with the following antibodies diluted with 10% normal goat serum as follows: anti-Flag (Sigma, F1804) 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Neuroscience 2024Quote: ... dorsal and ventral transverse slices were incubated with the following primary and secondary antibody combinations using the protocol described: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... with 200 μl of 15 μg/ml anti-CD63 antibody (Cosmo Bio, SHI-EXO-M02) or mouse IgG2a isotype control (Millipore, MABF1080Z) in PBS containing 0.02% Tween 20 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with the secondary antibodies Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma-Aldrich, DUO92002-100RXN) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue sections on Visium Spatial Gene Expression Slide were fixed with methanol at −20 °C for 30 min and labelled with an anti-αSMA antibody (Cy3 conjugated, 1:2000, Sigma C6198) for 30 min at room temperature followed by DAPI nuclear counter stain ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then rinsed 3 times for 10 minutes in PBS and incubated overnight at 4°C in a mix of the primary antibodies (mouse anti-S100β, Sigma Aldrich #S2532 ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with 5% BSA at room temperature for 1 hour and incubated with anti-FLAG M2-peroxidase antibody (Sigma-Aldrich) at room temperature for another hour ...
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bioRxiv - Immunology 2024Quote: ... cFibs cultured on Matrigel-coated cell culture plates were harvested and stained for Anti-Fibroblast Antibody Clone TE-7 (Millipore Sigma) and Antibody to Sarcomeric Myosin MF20 (University of Iowa Developmental Studies Hybridoma Bank ...
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bioRxiv - Plant Biology 2020Quote: ... GFP antibodies (Sigma) and Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate antibody were used ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibody (Millipore); and anti-mouse NeuN (1:1000 for 12 h ...
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bioRxiv - Neuroscience 2020Quote: ... SST antibody (Millipore, #MAB354 ...
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bioRxiv - Molecular Biology 2019Quote: ... S9.6 antibody (Millipore) was added and samples were incubated overnight at 4° C with gentle rotation ...
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bioRxiv - Microbiology 2022Quote: ... αFLAG antibody (Sigma) was used at 1:10,000 to detect Cap2-3×FLAG ...
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bioRxiv - Genetics 2024Quote: ... Antibody (CTCF: Millipore 07-729 ...
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bioRxiv - Physiology 2019Quote: The interactions between TRPA1 and IL-R1 in nodose ganglia cultures were detected by PLA with Duolink in situ kit (Sigma- Aldrich). Primary nodose ganglion cells were harvested and cultured on glass coverslips as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 105 IL-7 dependent primary Irf4−/− preB-I cell cultures were resuspended in 400 µL RPMI medium (D5030, Sigma-Aldrich) with 600 µL viral supernatant and 1.5 µL polybrene and spun in culture plates at 2700 RPM for 90 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: Fluorescent immunostaining for nuclear NFκB and cytoplasmic NFκB was quantified in immortalized C8D30 astrocytes following 2-hr treatment with 1μg/ml LPS or a cocktail of 3 cytokines: Il-1α (3ng/ml, Sigma, Cat# I3901), TNFα (30ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... the reporter cells were exposed to drugs or DMSO vehicle for one hour before the addition of 3 ng/mL IL-1a (Sigma, I3901), 400 ng/mL C1q (MyBioSource ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... PBMCs cultures not supplemented with IL-2 overnight were stimulated with 25 ng/ml phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... LP cells were incubated for 5 h with 20 ng/ml PMA and 1 μg/ml ionomycin in the presence of 10 μg/ml Berfeldin A (Sigma, IL). For FACS analysis ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA was isolated from the si-NC and si-Dnmt3aos-transfected M(IL-4) macrophage cells and was then treated with bisulfite using the Imprint DNA Modification Kit (Sigma-Aldrich). Bisulfite sequencing and pyrosequencing were conducted by Shanghai Sangon Biotech Corporation ...
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bioRxiv - Microbiology 2021Quote: ... All SEC-FPLC described in this study was performed on a GE Healthcare AKTA PV-908 FPLC system (Chicago, IL) on Superdex S200 10/300 GL (Sigma-Aldrich) and Superose 6 10/300 GL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Immunology 2022Quote: ... All cocultures were maintained in SCGM supplemented with IL-2 (120 IU/ml) and in the presence of 10 µM raltegravir (Sigma-Aldrich) and 0.4 µM nevirapine (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... virus supernatant was aspirated and preactivated PBMCs (0.5-0.8 x 106/well) in TexMACS medium supplemented with 100 IU/ml IL-2 and 6 ng/ml polybrene (Sigma-Aldrich) were added to each well ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2024Quote: The viral particle stock was diluted in sterile saline (0.9% NaCl) (Hospira, Lake Forest, IL) with 0.01% Fast Green (Sigma, St. Louis, MI). A 10-µl Hamilton syringe (Hamilton Company ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies were detected using fluorochrome-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:200 in 1% Triton-X100 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used are: tyrosine hydroxylase (TH) antibody (rabbit polyclonal, Millipore) for noradrenergic (NAergic ...
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bioRxiv - Cell Biology 2021Quote: ... Bound antibody was detected using peroxidase-conjugated secondary antibodies (Sigma-Aldrich Company Ltd ...
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bioRxiv - Molecular Biology 2021Quote: ... and Western blotting performed using CFTR antibodies or actin antibody (Millipore). The CFTR antibody utilized for full-length protein was 596 against amino acids 1204-1211 (UNC School of Medicine) ...
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bioRxiv - Biophysics 2022Quote: ... and mouse monoclonal primary antibody against myelin basic protein antibody (Millipore) at 1:200 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... GAPDH antibody (MAB374) and Cas9 antibody (MAC133) were purchased from Millipore. HSP72 (ADI-SPA-810 ...
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bioRxiv - Microbiology 2020Quote: ... β-actin antibody and C9 antibody were purchased from Sigma (A2228) and SANTA CRUZ (sc-57432) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against α-tubulin antibody (1:200; T6074, Sigma) and guinea pig polyclonal antibody anti-CenpC antibody (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2mM EDTA](IgG antibody: Millipore 06-371; SON antibody: abcam ab121759) and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody RNA-DNA hybrid (s9.6) antibody (Merck Millipore, #MABE1095, 3ul) or GFP (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM MgCl2) for 5-10 minutes at room temperature (∼25°C) before adding Monoclonal Anti-FLAG BioM2 antibody (Millipore Sigma, #F9291) and Qdot 705 Streptavidin Conjugate (ThermoFisher ...