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Citations for Millipore Sigma :
7801 - 7850 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Protein were separated by 8% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Millipore). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by SDS-PAGE and transferred onto a nitrocellulose filter membrane (Millipore, USA). The membranes were incubated with primary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and trypsin (porcine pancreas, Type IX-S, 13000-20000 BAEE units/mg protein, Sigma-Aldrich) stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES ...
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bioRxiv - Synthetic Biology 2020Quote: ... These pellets were resuspended and lysed in 100 µL of Bugbuster protein extraction reagent (Millipore) containing 5 U mL−1 Benzonase nuclease (Millipore ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred from gels to Immobilon-P polyvinylidene difluoride 0.45 μm membranes (Millipore, USA) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins samples were resolved by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore) for 16 h at 100 mA ...
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bioRxiv - Molecular Biology 2019Quote: RNA-renatured proteins (~40 μg) were supplemented with 1 μg anti-NF-H (N4142, Sigma), 1 μg anti-Tau (T9450 ...
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bioRxiv - Systems Biology 2019Quote: ... Expression of the CARD-CC proteins was determined with anti-Flag antibody (F-3165, Sigma). Expression of the E-tagged PKC clones with anti-E-tag antibody (ab66152 ...
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bioRxiv - Biophysics 2020Quote: ... We collect the supernatant and precipitate proteins by adding 300 mg/mL ammonium sulfate (Sigma). We stir the solution for 30 minutes at room temperature and centrifuge again at 12,000 rpm for 0.5 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... Eluted proteins were precipitated with the addition of 0.25x volume of 100% trichloric acid (Sigma). Protein pellets were washed five times with cold Acetone (Merck ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) with the mouse anti-Ago2 antibody (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 30K (Merck Millipore) and stored at -80°C.
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bioRxiv - Molecular Biology 2020Quote: ... coli ProQ protein or its N-terminal domain (NTD) were cloned into pET15b vector (Novagen) using BamHI restriction site (Supplemental Table S2) ...
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bioRxiv - Biophysics 2019Quote: ... followed by on-matrix cleavage of the protein using 50 units of bovine thrombin (Sigma) per litre of culture at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein co-immunoprecipitation was performed using ANTI-FLAG® M2 Affinity Gel (A2220, Sigma Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-Sepharose (P3391) and PRMT5:MEP50 active complex (SRP0145) were purchased from Sigma Aldrich. Protein co-immunoprecipitation was performed using ANTI-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Molecular Biology 2021Quote: ... The following commercially available antibodies were used to detect targeted proteins: anti-SPF45 (Sigma-Aldrich), anti-SF3b155 (MBL Life Science) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich) rotating at 4 °C for 2 hr ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore). The purity of the protein was analyzed by SDS-page and Western-blot using Anti-His antibodies.
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bioRxiv - Microbiology 2020Quote: ... The eluate containing protease and cleaved protein was concentrated using Amicon Ultra 10,000 MWCO (Millipore), then passed through a Superdex-200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
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bioRxiv - Microbiology 2021Quote: ... Total protein concentrations were determined using the bicinchoninic acid assay (Sigma Inc.; procedure TPRO-562), and SDS-PAGE (12% ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue lysates were precleared for 2 h with Protein A + G magnetic beads (EMD Millipore). Precleared lysates were then incubated with 5 μg of anti-Sox9 antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: The following proteins were detected by western blotting (antibodies in brackets) CPSF3 (Sigma-Aldrich, HPA04657), IntS11 (Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: ... Proteins were subsequently electrotransferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P Membrane, PVDF, Millipore) for 20 min at 25 mA and 2,5 mV in a Trans-Blot Turbo TM Transfer System (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins resolved by gel electrophoresis were transferred onto an Immobilon-FL PVDF membrane (EMD Millipore) at 100 Volts for 90 minutes in ice-cold transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to 42-μm-pore Millipore PVDF membranes (Sigma-Aldrich, MO) for 1 h at 30 V ...
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bioRxiv - Immunology 2020Quote: ... Eighty micrograms of protein was separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with milk or BSA for 1 h before incubation with primary antibodies for 1.5 h ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Immunology 2020Quote: ... and 10 mg of protein per experiment were reduced with 10mM dithiothreitol (DTT, Sigma, #D0632) for 1 hour at 55°C and alkylated with 20mM iodoacetamide (IAA ...
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bioRxiv - Genetics 2019Quote: Proteins were precipitated in 23% TCA (Sigma-Aldrich, St. Louis, MO, Product number T-0699) at 4 °C O/N ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing-grade modified trypsin (Sigma). To purify the peptides ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the purified proteins were concentrated with Amicon Ultra-0.5 Centrifugal Filter devices (Merck Millipore) following manufactures instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The eluted proteins were concentrated 5X with 10KDa-cutoff Amicon centrifugal filter unit (Millipore, #UFC501024), and analyzed by native gels and Western blotting.
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bioRxiv - Physiology 2019Quote: ... Data were normalized by protein content of the homogenate measured by Bradford assay (Sigma Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... followed by an overnight incubation with Pure Proteome Protein A/G mix magnetic beads (Millipore). Samples were washed as indicated by the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated using a 30 kDa MWCO centrifugal filters (Amicon Ultra; Merck Millipore) and then gel filtered ...
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was induced by 0.2 mM Isopropyl-²-D-thiogalactopyranoside (IPTG; Sigma-Aldrich, Taufkirchen, Germany) and continued at 16 - 20°C for 18 - 20 h ...
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bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Immunology 2021Quote: S protein at 1mg/ml in PBS was cross-linked with 4% formaldehyde (FA) (Sigma) overnight at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The remainder of the samples was incubated for 1hr with uncoated protein G beads (Millipore) to remove a-specifically bound proteins ...
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bioRxiv - Cell Biology 2021Quote: ... The protein sample was concentrated in an Amicon Ultra 15 centrifugal filter (Millipore Sigma, UFC900308) to a concentration 1mg/ml and injected into Guinea Pigs (Pocono Rabbit Farms and Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000 ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... The protein was eluted with buffer C (buffer B+10%glycerol+2.5 mM Desthiobiotin (Novagen)) in four column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of Clover-MgtA fusion proteins was induced by addition of 0.2 % L-arabinose (Sigma) in exponential growth phase for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified protein was concentrated with Amicon Ultra-0.5 mL centrifugal filters (50kDa cutoff; EMD Millipore) to 100 µL ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mg protein were incubated with ANTI-FLAG® M2 Affinity Gel (Sigma, cat.no. A2220) for 2 h on a rotator at 4°C ...