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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein was precipitated after chilling samples on ice and adding cold ammonium acetate (Sigma, A1542) to a final concentration of 1.875 mM ...
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bioRxiv - Neuroscience 2023Quote: ... Total proteins of BR-EVs were extracted with RIPA buffer containing 0.5% PMSF (Sigma Aldrich). In brief ...
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bioRxiv - Biophysics 2023Quote: ... Protein was eluted with wash buffer supplemented with 100 mg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to a storage buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins reacting with primary antibodies were visualized by the Immobilon forte western HRP substrate (Millipore) for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Microbiology 2023Quote: ... The protein bands were developed with Immobilon Forte Western HRP substrate (Millipore Sigma, MA, USA) and imaged using a Bio-Rad ChemiDoc Imaging System (Hercules ...
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bioRxiv - Microbiology 2023Quote: ... Dried protein pellets were resuspended with 50 mM TEAB buffer (Millipore Sigma, Burlington, MA, USA) and quantified using Pierce 660nm Protein Assay Reagent (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein’s peak fractions were pooled and concentrated using 50kDa cut-off Amicon (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were separated by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore).
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was pre-treated with EZview Red Protein A agarose beads (P6486; Sigma) for 45 minutes to reduce the likelihood of nonspecific binding to the agarose ...
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bioRxiv - Cell Biology 2022Quote: Proteins from GL261 and CT2A cells were extracted in RIPA buffer (Sigma, cat# R0278-50mL) containing a protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and the lysates were immunoprecipitated with specific antibody and protein A/G-Sepharose (Sigma-Aldrich) at 4 °C for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was extracted using radioimmunoprecipitation (RIPA) assay buffer (Cat # R0278, Sigma-Aldrich, St. Louis, MO) containing cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... The expressed protein was purified using Glutathione Sepharose 4B (Sigma-Aldrich Cat# GE17-0756-01) using the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Western blotting of immunoprecipitated proteins was performed as described above using anti-FLAG (F1804, Sigma) or anti-HA (11867423001 ...
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bioRxiv - Biochemistry 2023Quote: ... and protein thereafter eluted with Strep-buffer supplemented with 2.5 mM d-desthiobiotin (Sigma-Aldrich). All purified proteins were concentrated to appropriate concentration using 30 kDa Mw cut-off centrifugal filters (Amicon ...
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bioRxiv - Cancer Biology 2023Quote: RIP experiment was optimized using the Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, USA) in light of the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Physiology 2023Quote: ... 15-35 µg protein per sample were denatured with 5 % v/v 2-mercaptoethanol (Sigma) in LDS sample buffer (Thermo ...
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bioRxiv - Zoology 2023Quote: ... 10 μl of supernatant was used for protein quantification using Bradford Reagent (Sigma, B6916-500ML). Whole-body uric acid levels were measured from 5 μl of supernatant at 22°C for 30 min using uric acid assay kit following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was purified using the HiPrep Q Fast Flow 16/10 column (Sigma-Aldrich) followed by the reverse-phase HPLC C4 column ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... Isolated proteins were then segregated by gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). Membranes were subsequently incubated in primary antibodies (Supplementary Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... Purified proteins were further concentrated and changed to PBS buffer using the Centricon device (Millipore).
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bioRxiv - Neuroscience 2023Quote: Total protein samples were lysed in radioimmunoprecipitation (RIPA) buffer containing 2% protease inhibitor cocktail (Sigma). Protein quantification was completed using the Pierce Bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by 10% SDS-PAGE and transferred to the PVDF membrane (Millipore, USA). The membrane was placed in 5% bovine serum albumin and incubated with the anti-midP0188 pAb (1:500 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... TEV digested protein was concentrated to < 2 mL using an Amicon Ultra-15 concentrator (Millipore) and then purified by size exclusion chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 1 h incubation with Protein G agarose beads (Millipore, catalog no. 16-266) at 4 °C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... The separated protein samples were transferred to PVDF membranes (Immobilon-P; Millipore, Burlington, MA, USA) using the Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated protein (7-15 mg/ml) was serially diluted in 1% Genapol X-100 (Sigma). 0.3-0.5 μl of diluted protein was added to the cis (ground side ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were purified from clarified supernatants using a 1mL StrepTrap™ HP column (Sigma), buffer exchanged ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 0.5ml using Amicon Ultra-4 ml Centrifugal Filters (Millipore) and loaded onto a gel filtration column (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then added with 75 μl pre-washed Protein A Sepharose (Sigma, #P6649) and incubated at 4 °C for 30 min with rotation ...
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bioRxiv - Plant Biology 2023Quote: ... antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma) antibody for AVR-Pii and ZiF effectors in pGADT7.
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bioRxiv - Biochemistry 2023Quote: ... Polyubiquitinated proteins were visualized by immunoblotting for ubiquitin (Millipore Sigma 05-994, clone P4D1-A11) and actin was used as loading control (Millipore Sigma MAB1501R ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were detected by Western blotting using the following primary antibodies against: Flag (M2, Sigma), ERK1/2 (3A7 ...
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bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... beads were incubated directly with 4X protein loading buffer (Li-Cor) with 2-Mercaptoethanol (Sigma). For digital PCR ...
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bioRxiv - Neuroscience 2023Quote: ... The fractions were then pooled based on protein concentration and Amicon centrifugal filters (EMD Millipore) were used to concentrate the samples ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were loaded into Amicon® Ultra-15 centrifugal filter unit (Millipore Sigma), washed with Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were detected with ECL Prime Western blotting Amersham (Sigma Aldrich, cat. no GERPN2232) detection reagent and images acquired with STELLA imaging system ...
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bioRxiv - Immunology 2023Quote: ... protein bands within the gel were transferred on an Immobilon-FL transfer membrane (Millipore IPFL00010) for 45 mins at 20 volts and 400 mAMPs ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was eluted with a cocktail of 100 ug/ml RNase A (Sigma-Aldrich) and 0.1 units/ µL RNase H (Epicenter) ...
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bioRxiv - Cancer Biology 2023Quote: ... All the proteins were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore Corporation, UFC901024).
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bioRxiv - Developmental Biology 2023Quote: ... Medium was supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Sigma) when iPSCs were thawed or passaged as single cells.