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Citations for Millipore Sigma :
7751 - 7800 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The proteins were then transiently transfected (pBiex-1; Escort IV, Sigma-Aldrich, St. Louis, MO) into Sf9 cells ...
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bioRxiv - Cancer Biology 2022Quote: Total protein extracts were obtained from cell lines and tumour specimens using RIPA (Sigma-Aldrich) buffer and subjected to SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were stained with Ponceau S (0.1% Ponceau reagent [Sigma, P3504] with 5% acetic acid). Incubation with primary antibodies was conducted overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... to be subsequently treated with Iodacetamide for protein alkylation (Sigma-Aldrich, St. Louis, MO, USA) and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were reduced with 60 µL 40 mM dithiothreitol (DTT; Sigma-Aldrich, Taufkirchen, Germany) in 50 mM TEAB ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Cell Biology 2021Quote: ... crosslinked to Protein A/G PLUS-Agarose (SantaCruz) using 20 mM dimethyl pimelimidate (Sigma Aldrich) at 4°C for overnight ...
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bioRxiv - Cell Biology 2021Quote: ... protein was dialyzed into REMSA buffer [10mM HEPES pH 7.3 (Sigma-Aldrich, Cat. No. H3375), 20 mM KCl (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were then electro-transferred onto a polyvinylidene fluoride (PVDF) membrane (0.2 µm; Millipore). After blocked with 5 % skim milk in TBST (50 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Japan) and anti-glial fibrillary acidic protein (GFAP) (1:2,000; mouse monoclonal, G3893, Sigma-Aldrich) were used to assess activated microglia and reactive astrocytes in ischemic regions ...
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bioRxiv - Microbiology 2021Quote: ... After preclearing the cell lysate with protein A/G agarose magnetic beads (#16-663, Millipore) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Chloroquine diphosphate (C6628) and HRP-conjugated Protein G (#18-160) were purchased from Sigma-Aldrich. Torin1 (#4247 ...
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bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... separated protein lysates were transferred onto polyvinylidene difluoride membranes (Immobilon-P; EMD Millipore, Burlington, MA); membranes were blocked in 5% BSA (BSA Fraction V ...
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bioRxiv - Neuroscience 2021Quote: ... and the proteins denatured by adding 200 μL of 8 mol/L urea (Sigma-Aldrich), followed by 15 min sonication ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Biophysics 2020Quote: ... The purified protein was incubated and for 1h at 37°C with bovine thrombin (Sigma) in a 1000:1 molar ratio ...
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bioRxiv - Cancer Biology 2021Quote: ... After preclearing the cell lysate with protein A/G agarose magnetic beads (16-663; Millipore) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... fractions containing pure protein were concentrated using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma), dialyzed into PBS ...
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bioRxiv - Biochemistry 2020Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 30°C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... was used for blotting and proteins were stained using the following antibodies: (Sigma-Aldrich AV46863), TMEM41B (Sigma-Aldrich HPA014946) ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of total protein in the samples were quantified using the Bradford assay (Sigma). An equal concentration of protein samples was mixed with 2X Laemmli buffer and the samples were boiled for 10 min at 100° C ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Molecular Biology 2022Quote: ... and the ATAD5-RLC proteins were eluted with 100 µg/mL FLAG-peptide (Sigma Aldrich) in the same buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was transferred from the 20% polyacrylamide gels to PVDF Immobilon-P membranes (Millipore Sigma) and blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: Tissue proteins were extracted according to kit instructions (Sigma Aldrich, R0278, St. Louis, Missouri, USA) and protein concentrations were routinely quantified by using a BCA kit (Beyotime ...
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bioRxiv - Immunology 2022Quote: ... After that 50 µl of 1:5 dilution Protein A colloidal gold conjugate (Sigma, USA), which is used as an indicator was added to the test hole and was allowed to soak for 2 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were transferred into new tubes and protein concentration were determined by BCA assay (Sigma). Approx ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto a nitrocellulose membrane (Sigma-Aldrich). After incubation with the appropriated primary ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to 1 mg/ml using a 50,000 MWCO Amicon Concentrator (Millipore), flash frozen and stored at -80°C.
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bioRxiv - Biochemistry 2022Quote: ... proteins separated by SDS-PAGE were transferred to a PVDF membrane (Immobilon-P, Merck Millipore) in a Mini Trans-Blot (Bio-Rad ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... the proteins on the gel were transferred to Immobilon PVDF membranes (EMD Millipore, Billerica, MA) at 400 mA for 2.5 hrs ...
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bioRxiv - Physiology 2022Quote: Protein samples were solubilized in 1% NP-40 lysis buffer with 4% protease inhibitors (Sigma) and centrifuged for 14,000 g for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: Antibodies used were: rat anti-myelin basic protein (MBP, 1:200, MAB386, Millipore, MA, USA), rabbit anti-Olig2 (1:200 ...
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bioRxiv - Cancer Biology 2019Quote: ... The DTT and IAA treated proteins were then dialyzed against 50 mM ammonium bicarbonate (Sigma) for 24h at 4C and with the dialysis buffer changed three times ...
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bioRxiv - Plant Biology 2020Quote: ... Solubilized proteins were incubated with 15-20 µl of Anti-FLAG M2 Affinity Gel (Sigma) for 2 h with gentle rotation at 4°C ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2019Quote: Protein samples were separated by SDS-PAGE and blotted on PVDF (polyvinylidenfluoride) membranes (Merck Millipore). Membranes were blocked for 1h with PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Firefly luciferase activity was normalized to protein content using Bicinchoninic Acid Kit from Sigma-Aldrich® and measured with using a luminometer (Centro XS3 LB960 ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2019Quote: ... incubated with mouse α-anti-HA clone 12CA5 conjugated with protein G beads (Sigma-Aldrich) for 16 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were removed using Amicon Ultra 3K 0.5 ml centrifugal filters (Millipore, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... buffer exchanged and protein dialysis was performed using Amicon Ultra-4 centrifugal concentrators (UFC801008, Millipore,) with a molecular weight cut off of 10 kDa and the final concentration was estimated using the Bradford protein assay (5000006 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred into methanol pre-activated polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck-Millipore). After transference ...
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bioRxiv - Immunology 2019Quote: ... Protein aliquots were reduced with 5 mM tris-2-carboxymethyl phosphine (TCEP) buffer (Sigma Aldrich) and incubated for 1 hour at 60°C to reduce disulfide bonds ...
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bioRxiv - Plant Biology 2020Quote: Sample proteins were separated by SDS-PAGE and electrotransferred onto an Immobilon-P membrane (Millipore) for immunoblot detection ...
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bioRxiv - Immunology 2019Quote: ... Eluted proteins were concentrated using Amicon Ultra 0.5ml Centrifugal Filter tubes NMWL 30 KDa (Millipore) and quantified by NanoDrop Spectrophotometer (Thermo Fisher) ...