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Citations for Millipore Sigma :
7601 - 7650 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Systems Biology 2024Quote: The MIA PaCa-2 pancreatic cancer and HEK 293 human embryonic kidney were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma Aldrich, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... hydrogel used within the arrays was previously developed to mimic the material properties of human mucus.29 A solution of 4% porcine gastric mucins (PGM; Sigma Aldrich; mucin from porcine stomach ...
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bioRxiv - Genetics 2023Quote: ... Control female mice were injected with 5 U of pregnant mare serum gonadotropin (PMSG, nor272-Prospecbio, Israel) and 46 h later injected with 5 U of human chorionic gonadotropin (HCG, C1063 Sigma-Aldrich) and placed into the home cage of the male to mate overnight ...
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bioRxiv - Immunology 2023Quote: ... and maintained at 37°C with 5% CO2 in complete RPMI in the presence of 5% human AB serum (Sigma-Aldrich) for differentiation into MDMs for 10-14 day (Pierini et al. ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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bioRxiv - Microbiology 2024Quote: The human monocytic cell line U937 (ATCC CRL-1593.2) was obtained from ATCC and primary human peripheral monocytic cells (PBMCs) were purchased from Sigma (HUMANPBMC-0002644). U937 cells were cultured in RPMI medium (Genesee ...
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bioRxiv - Microbiology 2024Quote: ... Primary human PBMC were differentiated into macrophages (hMDMs) after culturing for 7 days with RPMI supplemented with 20% heat-inactivated human serum (Sigma-H4522).
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bioRxiv - Cell Biology 2024Quote: ... the media were switched into Phase II erythroid differentiation media consisting of Stem Span SFEMII supplemented with 3% normal human serum (Sigma-Aldrich), 3 IU/ml erythropoietin (R&D Systems ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 5 U thrombin increments were added to microcentrifuge tubes containing 50 µl of human citrated plasma (Sigma Cat No. P9523-1ML), the time to clot formation was recorded ...
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bioRxiv - Bioengineering 2024Quote: To test the micromechanical properties of meniscal cells and newly synthesized PCM, 35-mm petri dish (351008, Life Sciences) was treated with 20 µg/mL human fibronectin (10838039001, Sigma-Aldrich) for 12 h prior to seeding meniscal cells with an initial density of 5,000 cells per dish ...
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bioRxiv - Microbiology 2024Quote: ... by human neutrophils was measured over a time of 30 minutes by monitoring luminol-amplified chemiluminescence using 282 μM luminol (Sigma Aldrich). Neutrophils were stimulated either with negative control (Hank Balanced Salt Solution ...
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bioRxiv - Cell Biology 2024Quote: ... The shRNA plasmids were obtained from the human genome-wide TRC shRNA library (purchased from Sigma-Aldrich, USA, Catalog no. SH0411), available at shRNA resource centre ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were alkylated by addition of 100 μl of 50 mM iodoacetamide (Sigma RPN6320V) prepared fresh in 50 mM NH4HCO3 pH 7.5 buffer and allowed to react in the dark at 20 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted kinases (proteins) were concentrated with Amicon Ultra-4 (10K cutoff) spin columns (Millipore), purified by removing the detergent using methanol/chloroform extraction ...
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bioRxiv - Cell Biology 2020Quote: ... PreScission protease-eluted supernatant was added to S-protein agarose beads (EMD Millipore, Burlington, MA) and incubated rocking for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection was performed using chemiluminescent substrate (ECL™ Western Blotting Reagents, Sigma-Aldrich, GERPN2106) with film exposure (HyBlot CL® Autoradiography Film ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated with Amicon Ultra-15 Ultracel 10k centrifugal filter units (Merck Millipore, #UFC901024). Purification of cytoplasmic actin ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against cytoskeletal proteins (vimentin, actin and β-tubulin were from Sigma (St.Louis, MO, USA). The mitochondrial marker MitoTracker® ...
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bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
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bioRxiv - Immunology 2021Quote: ... Separated proteins were transferred to a nitrocellulose membrane (GE 10600002) or PVDF membrane (Millipore IPVH00010). Membranes were blocked for a minimum of 0.5 hour at room temperature in a 5% (w/v ...
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bioRxiv - Immunology 2021Quote: The 96-well polystyrene microplates were coated with 500 ng of protein A (Sigma-Aldrich) in coating buffer for four h at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: Protein extracts were prepared with RIPA buffer supplemented with protease and phosphatase inhibitors (Sigma-Aldrich) using a minipestle or Bioruptor Pico (Diagenode) ...
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bioRxiv - Molecular Biology 2020Quote: ... was filtered through a frit filter plate (96-Well Protein Precipitation Filter Plate, Sigma Aldrich), and the effluent was collected and evaporated to dryness and the residue was dissolved in 20 ▯L of a 40:60 MeOH:H2O v/v mixture containing the same 13C-PFAS injection standards ...
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bioRxiv - Microbiology 2019Quote: ... the DNA sequences encoding the proteins without the signal sequence were cloned into pET28a (Novagen) using the NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoreactive protein signals were visualized using stable peroxide solution and luminol/enhancer solution (Millipore, USA). Silkworm α-tubulin protein was used as an internal control.
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bioRxiv - Evolutionary Biology 2021Quote: ... triglyceride assays (Pointe Scientific T7532-120, T7531-STD) and soluble protein assays (Sigma B9643-1L) were then carried out in triplicate according to the kit instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein lysis was incubated on ice with 50 U of Benzonase Nuclease (Millipore, #E1014-5KU) for 20 min and at 37 °C for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Heart lysates were first pre-cleared with 20 μl of packed protein A beads (Sigma) per lysate on a rocker or tumbler at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Physiology 2022Quote: ... Oocytes expressing desired proteins were harvested in ice-cold CelLytic M lysis buffer (Sigma-Aldrich) supplemented with proteinase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were eluted in HBS containing 1 mM DTT and 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
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bioRxiv - Neuroscience 2021Quote: ... 100 μg of total protein for each group were reduced with 5 mM dithiothreitol (Sigma) for 30 min at 56 °C and then alkylated with 11 mM iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... protein-A sepharose and ANTI-FLAG® M2 Affinity Gel beads (Sigma Aldrich, Cat# A2220) were washed three times with 1x PBS and two times with IP100 buffer (25 mM Tris-HCl 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to a 0.45 µm PVDF membrane (EMD Millipore, Billerica, MA, USA), and the blot was probed with anti-AGO2 mouse monoclonal antibody (Wako Pure Chemical Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins by SDS-PAGE were transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore), washed with TBST for 5 minutes and then were blocked with 5% BSA in TBST[0.1%(v/v ...
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bioRxiv - Molecular Biology 2021Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 4°C for 1 hour with rocking ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto a polyvinylidene fluoride membrane (Immobilon R, Merck Millipore Ltd) for 1 hour at 20 V ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was transferred to a new tube and protein concentration determined by Direct Detect (Millipore). 1mg of lysate per immunoprecipitation was used for the following steps ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Protein degradation was triggered by addition of 0.5 mM indole-acetic acid (IAA; Sigma-Aldrich) to the growth medium.
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bioRxiv - Biophysics 2019Quote: ... the protein sample was filtered using a 30 kDa centricon filter (Merck Millipore, Darmstadt, Germany). The crude protein was then injected into a DEAE anion exchange column equilibrated with 10 mM Tris (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... Immunoblotting was performed with anti-Protein A mouse monoclonal primary antibody (SPA-27, Sigma Aldrich) or anti-LukS-PV mouse monoclonal primary antibody (IBT) ...