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Citations for Millipore Sigma :
7751 - 7800 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 16-h incubation in PBS with 0.1% Triton™ X-100 with the following antibodies: mouse monoclonal anti-NeuN (1:500; EMD Millipore, MAB377), rabbit polyclonal anti-KCC2 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... 600 μl aliquots of diluted chromatin were incubated with 7 μl of the rabbit anti-trimethyl-Histone H3 (Lys27) antibody (Millipore, 07-449) in a rotator at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then washed and stained with 1:100 dilution of mouse anti-sarcomeric α-actinin primary antibody (A7811, Sigma-Aldrich) and 1:100 rabbit anti-CD31 (AB32457 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with 5% BSA in 0.1% TBS-Tween for 1 hr at room temperature for blocking step and incubated with 1:1000 anti-RFC1 antibody produced in rabbit (Sigma-Aldrich; AV44167) at +4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Microtubules were linked to PEG-silanized coverslips by incubating chambers with 33 μg/mL monoclonal anti-beta-tubulin III (neuronal) antibody (Sigma-Aldrich, T8578) for 5 minutes prior to washing with 10 mg/mL BSA for 2 minutes ...
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bioRxiv - Immunology 2023Quote: ... To ensure efficient and reproducible protein immobilization a preliminary array validation was carried out with anti-FLAG antibodies (Sigma-Aldrich, cat# F7425) 1:5000 and mouse anti-His6 tag polyclonal antibodies (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... with 0.2% triton X-100 and for 12 h at 4 °C in a humidified chamber with the primary antibody: mouse-anti-NeuN (Neuronal Nuclei, Sigma-Aldrich MAB377) or goat-anti-MMP-9 (Matrix metallopeptidase 9 ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with bovine serum albumin (0.5%) before labeling with anti-STIM1 monoclonal antibody (1:50 dilution; 30 min; Sigma-Aldrich, Rockford, IL). Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Bioengineering 2023Quote: ... Blockade of collagen VI-integrin interactions was performed using monoclonal antibodies against integrin β1 (10 μg/mL anti-integrin β1, Millipore sigma, MAB2253), Cilengitide (5 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were then transferred from the 96-well plates into opaque white 384-well plates that were pre-coated with monoclonal anti-FLAG M2 antibody (Millipore Sigma, F1804). Plates were blocked with 1% BSA/5% sucrose/0.5% Tween 20/1xPBS ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C in blocking solution with the addition of the following antibodies (dependent on experiment): goat anti-ChAT (Millipore Sigma AB144P), rabbit anti-cFos (Synaptic System 226 008) ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 hours at RT and then immunoblotted overnight at 4°C with the primary mouse anti-amyloid precursor protein A4 antibody (clone 22C11) (Sigma MAB348-AF647) (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed 3 times with PBS and incubated with blocking solution (PBS/BSA 1%) for 1 h at 37 °C and then with the following primary antibodies: mouse anti-GFAP (1:500; Millipore, Cat. MAB360), rabbit anti-C3 (1:25 ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with rabbit-anti-Iba-1 primary antibody (1:1000, Wako Chemicals; Cat. No) and WFA (1:400; Sigma; Cat. No) for 23 hours at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated in secondary antibody (Alexa Fluor 488 Goat anti-Rabbit, 1:500, Invitrogen #11008; DAPI, 1:1200, Sigma- Aldrich #D9564) at 4°C in the dark overnight with rocking ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL of protein extract were loaded on a denaturing polyacrylamide gel before being transferred to a nylon membrane and probed with an anti-flag M2 antibody (Sigma Aldrich F1804). An anti-β-actin antibody (Sigma Aldrich A2066 ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess serum was tipped off and sections were incubated with primary antibody (anti-FLAG, Sigma F7425, 1:100 diluted in 1% BSA), overnight in a humid chamber at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in TBS-T and incubated overnight at 4 °C in the same buffer with anti-TH monoclonal antibodies (1:500; Millipore, Stockholm, Sweden). Immunoreactivity was detected using a secondary antibody conjugated with Alexa fluorescent dye (1:400 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then permeabilized by addition of ice-cold 70% ethanol and stained with an anti-H3S10ph antibody (Merck-Millipore, 05-1336) diluted 1:1000 for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... blotted and incubated overnight in 5% skim milk (GERBU Biotechnik, 70166) in TBST at 4°C with an anti-RAS antibody (Millipore, 05-516). As a loading control ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in antibodies for 48h at 4 °C in a blocking solution and immunolabeled with chicken anti-Nefh (1:5000, EMD Millipore, AB5539), mouse anti-Syn1 (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibodies (POLR2A, Abcam monoclonal antibody, ab5408; SMN, Santa Cruz polyclonal antibody H-195, sc-15320; ACTB, Sigma, A5441) were used at 1:2,000 to 1:5,000 dilutions for incubation overnight followed by three times washes with RIPA buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The horseradish peroxidase (HRP) secondary antibodies polyclonal antibody against FLAG and polyclonal antibody against HA were from Sigma-Aldrich. All secondary antibodies conjugated with Alexa Fluor dyes for immunostaining were from Life Technologies.
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bioRxiv - Biophysics 2019Quote: ... The following antibodies and respective secondary antibodies were used in the indicated dilutions: polyclonal FN or LN antibodies (Sigma-Aldrich, St ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... GFP antibodies (50430-2-AP, Proteintech; MAB3580, Millipore; Antibody Facility MPI-CBG) 1:250 and Histone H3 (phospho S28 ...
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bioRxiv - Plant Biology 2019Quote: ... primary antibodies and secondary antibodies coupled to Horseradish Peroxidase (HRP, Sigma Aldrich) for protein detection on blots.
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bioRxiv - Biochemistry 2020Quote: ... OC antibody of 1:25,000 and A11 antibody of 1:500 (Millipore and Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... samples were treated with SP_1505 antibody or control rabbit IgG antibody (Sigma) at concentrations indicated in figure legends ...
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bioRxiv - Plant Biology 2019Quote: The antibody fractions of the polyclonal HRP antibody mixture (P7899, Sigma-Aldrich) binding to α1,3-fucose and anti β1,2-xylose ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated in antibodies against acetylated tubulin antibody (Sigma, 1:1000) and SV2 (DSBH 1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... The antibodies used were: rabbit antibody against Alix (1:500; ABC40, Millipore), mouse monoclonal against CNP (1:1000 ...
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bioRxiv - Pathology 2021Quote: ... Antibodies used were a rabbit polyclonal antibody to podocin (Sigma-Aldrich, P0372) used at a dilution of 1:100 ...
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bioRxiv - Physiology 2022Quote: ... The BCKDK antibody (AV52131) and AUH antibody (AV40374) were purchased from Sigma. Anti-PPARγ (#81B8) ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were alkaline phosphatase-coupled IgG antibodies (1:10000; Sigma-Aldrich).