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Citations for Millipore Sigma :
7901 - 7950 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with PBS to remove residual puromycin followed by staining of cells using Alexa fluor-488 anti-puromycin antibody (Millipore, Cat#MABE343-AF488).
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bioRxiv - Neuroscience 2022Quote: ... sections were blocked for 1 hour in 10% normal goat serum (at room temperature) and probed with an anti-NeuN Alexa Fluor 488 conjugated antibody (1:100; Millipore-Sigma cat. # MAB377X) for 2 hours at room temperature and protected from light ...
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bioRxiv - Genetics 2022Quote: ... For normalization of signals, we used actin or Pgk1, which was detected by using mouse anti-actin monoclonal antibody (1:5000, (Millipore Cat# MAB1501, RRID:AB_2223041)) or mouse anti-Pgk1 antibody (1:10000 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Cultured human cortical neurons of 100-120 days post-differentiation were immunostained as above with primary antibodies against MAP2 (Mouse-anti-MAP2, Sigma, 1:200 dilution), VGLUT1 (Mouse-anti-VGLUT ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Genomics 2019Quote: ... the membrane was incubated with properly diluted primary antibody (M2 Monoclonal ANTI-FLAG 1:1000, F1804, Sigma; Monoclonal GAPDH 1:5000, 2118S, CST) in 5% dried milk/TBST overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed three times in 1x TBST and then incubated for 1 hour at 22°C in anti-mouse IgG HRP conjugate secondary antibody (Sigma, 2834819; 1:1000). Membranes were washed three times in 1x TBST before adding enhanced chemiluminescence detection reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... blocked in 3% BSA followed by overnight staining with lectin GS-II-Alexa594 and an anti-αSMA-alexa488 antibody (1:150, Sigma, in-house conjugated) in 0.5% triton in PBS ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates from different groups were collected and incubated with magnetic bead RIP buffer containing anti-human argonaute 2 (Ago2) antibody (Millipore, Billerica, MA, USA) and anti-human CPEB2 antibody (Proteintech ...
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000, EMD Millipore, #AP132B, RRID:AB_11212148) followed by 1 h with avidin-biotin complex (ABC Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Pathology 2020Quote: ... The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA, cat. no. A5228; 1:500; Sigma-Aldrich), anti-vimentin (cat ...
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bioRxiv - Neuroscience 2020Quote: ... We incubated free-floating CeA coronal sections (16 µm) for 2 h at 30 °C with rabbit anti-FG antibody (Millipore; AB153I; 1:200) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Biochemistry 2020Quote: ... 10 µL of cell samples were transferred into a 96 well assay plate and incubated for 20 min in the dark at 4°C with 2 µL 7-amino-actinomycin D viability staining solution (eBioscience, catalog #00-6993-50) and 10 µL anti-HA FITC-conjugated antibody (Sigma-Aldrich, catalog #H7411), the latter pre-diluted 1:100 in TBS+4% BSA ...
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bioRxiv - Neuroscience 2021Quote: Brains of male and female mice expressing EYFP in MC3R neurons were collected and processed as describe above and neurons expressing TH were labeled using standard immunohistochemical (IHC) techniques (as described above for c-fos) using mouse anti-TH antibodies (Catalog # MAB318, Millipore, Inc., Billerica, MA) diluted 1:1500 followed by Alexa Fluor 594 conjugated goat anti-mouse antibodies (Catalog # A-11032 ...
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bioRxiv - Cell Biology 2021Quote: ... Human-αSMA staining was visualised by 1h incubation with a secondary goat anti-rabbit-HRP conjugated antibody followed by a 10 min incubation with 3,3’-diaminobenzidine (DAB Fast Tablet, Sigma-Aldrich, St. Louis, MO). Nuclear counterstaining was performed with hematoxylin after which the sections were dehydrated and mounted with Entellan.
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from fresh frozen pulverized postmortem brain tissue using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Neutralization assays were performed by pre-incubating cells with anti-GalactosylCeramide antibody (mGalC, 10µg/mL, Millipore 10µg/mL, 30 min at 4°C) or viral inoculum with monoclonal antibody against HIV-1 gp120 (1-2µg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
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bioRxiv - Developmental Biology 2020Quote: ... permealized in 0.1% Triton X and 5% goat serum in PBS for 1 hr and incubated with primary antibody (anti-FLAG (Sigma, F1804, 1:300), anti-OCT4 (R&D ...
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bioRxiv - Genetics 2020Quote: ... samples were stained overnight at 4°C with anti-Olig2 antibody conjugated to Alexa Fluor 488 (cat no. MABN50A4, EMD Millipore, Burlington MA), anti-PU.1 antibody conjugated to Alexa Fluor 647 (cat no ...
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... CBX2 protein was expressed in Sf9 cells using the baculovirus system and affinity purified from nuclear extracts using agarose beads coupled with the M2 anti-FLAG antibody (Sigma-Alrich, Cat# A2220). Purified CBX2 protein (1 mg ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Plant Biology 2022Quote: ... a 1:5000 dilution of anti-rabbit IgG secondary antibodies was employed and detected by chemiluminescence with the Luminata pack (Millipore, Billerica MA, USA).
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at room temperature and incubated overnight at 4 °C with mouse anti-FLAG monoclonal antibody (Catalogue no. F3165, Millipore Sigma, MO, USA) diluted 1:1000 in Milk/PBST ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated for 1 h at room temperature with sheep anti-mouse horseradish peroxidase (HRP)-conjugated antibody (Catalogue no. GENA931, Millipore Sigma, MO, USA) diluted 1:10000 in Milk/PBST ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... in PBS (blocking buffer) was used to block the cells for one hour and replaced with anti-FLAG antibodies (Sigma Aldrich, F-1804) diluted in blocking buffer (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Neuroscience 2023Quote: ... eYFP fluorescence was used to confirm TH+ expression in VTA cell bodies using a rabbit anti-TH antibody (1:1000; Millipore Sigma, Burlington, MA) as the primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cell bodies were identified by immunolabeling against Glutamine Synthetase using rabbit anti-Glutamine Synthetase antibody (1:500, Sigma-Aldrich catalog# G2781, RRID:AB_259853), and GABA immunoreactivity was detected by immunostaining with guinea pig anti-GABA antibody (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... Washes were followed by the addition of 100 μL of anti-FLAG horseradish peroxidase (HRP)-linked monoclonal antibody (1:10,000 mAb, #A8592, Sigma Aldrich, St. Louis, MO) for 1 h at room temperature with rocking ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2024Quote: ... A housekeeping protein was also detected using the same blot by mild stripping and re-probing the membrane with an anti-GAPDH rabbit polyclonal antibody (Sigma-Aldrich, G9545-200).
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bioRxiv - Pathology 2024Quote: ... The membranes were incubated after washing with an anti-rabbit IgG alkaline phosphatase secondary antibody (1:5000, Sigma– Aldrich, St Louis, MO, USA) for 1 h ...
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bioRxiv - Immunology 2024Quote: ... and cultured at density 1.25×106 cells/ml in wells coated with anti-CD3 antibody (0.5 μg/ml; OKT3, Sigma-Aldrich, Saint Luis, Missouri, USA), in RPMI medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: The sample was incubated overnight (12Lhours) at 4 °C with a primary mouse anti alpha- and beta-tubulin IgG antibodies (Sigma-Aldrich (REF: T5168) and Sigma-Aldrich (REF ...
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bioRxiv - Neuroscience 2023Quote: ... the dye hFTAA59 was used at dilution of 1:400 in combination with the Cy3-conjugated monoclonal anti-α-smooth muscle actin antibody (Sigma Aldrich, C6198) at dilution of 1:800.
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bioRxiv - Microbiology 2024Quote: ... Thereafter the cells were incubated at 4 °C overnight in blocking-solution containing the primary antibody (Rabbit anti-hemoglobin (SIGMA, Cat. No. H4890) diluted 1/1000) ...