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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit anti-chicken and anti-sheep were purchased from Sigma.
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bioRxiv - Developmental Biology 2019Quote: ... Tissues were blocked in 5% normal donkey serum before an overnight incubation with the primary antibody at 4°C (1:200 for ICSBP antibody, sc-365042, Santa Cruz; and 1:100 for MEF2C antibody, SAB4501860, Sigma-Aldrich). The slides were washed twice in PBS at room temperature and applied with secondary antibody diluted 1:200 in 1% BSA prepared in PBS (biotinylated anti-mouse IgG (H+L) ...
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bioRxiv - Cell Biology 2019Quote: ... the captured proteins were eluted by 20 µl 2× SDS-PAGE loading dye and detected by western blot using anti-Flag antibody (Sigma, 1:3000, Cat# F1804).
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Puro-treated samples were then incubated for 1 hour with anti-puromycin Alexa Fluor 647 directly conjugated antibody (1:100; Millipore Sigma cat. no. MABE343). OPP-Puro samples were processed for Click-chemistry detection with Alexa Fluor 647 picolyl azide as per the instructions in the Click-iT® Plus OPP Protein Synthesis Assay Kit (Invitrogen cat ...
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bioRxiv - Biophysics 2019Quote: ... chemically fixed and permeabilized cells were labeled with anti-phosphotyrosine 4G10 Platinum mouse monoclonal antibody of the IgG2b subclass (Millipore Cat# 05-1050, RRID:AB_916371). The primary antibody was detected using a secondary goat anti mouse IgG Fcγ subclass 2b antibody (Jackson ImmunoResearch Labs Cat# 115-005-207 ...
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bioRxiv - Neuroscience 2019Quote: ... One mid rostro-caudal IC section from each animal was immunolabelled for GABA (rabbit anti-GABA antibody 1:1000, Sigma-Aldrich Cat# A2052, RRID:AB_477652), and the neuronal marker NeuN (mouse anti-NeuN antibody 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed in TBST 2 × 15 min and incubated for 1 hour in room temperature with HRP-conjugated anti-rabbit antibody (Sigma-Aldrich, NA9043, 1:2,500) diluted in TBST with 5 % non-fat dry milk ...
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bioRxiv - Neuroscience 2020Quote: ... antibody was used as a pan-neuronal marker and membranes were reprobed with mouse monoclonal anti-β-actin antibody (1:10,000; Sigma, Saint-Quentin Fallavier, France) to confirm equal protein loading ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins bound to beads were resolved on an SDS-PAGE and the presence of Nck was determined by immunoblot analysis using anti-Nck antibody (Millipore #06-288; 1:1000). As a negative control anti-Grb2 (BD Transduction #610112 ...
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bioRxiv - Physiology 2020Quote: ... Cells were incubated at RT for 3 hrs in 300 μl secondary antibody solution containing anti-rabbit TRITC (Sigma-Aldrich Cat# T6778, RRID: AB_261740) at a concentration of 1:75 in 1× TBS ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated for 24 hours at RT on a rocking platform with ATTO647N-conjugated anti-rabbit antibodies (Sigma-Aldrich, catalog no. 40839) diluted to 1:250 in the antibody dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin antibody (Lysine-40) (Sigma Clone 6-11B1), α-tubulin (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the bound (B) and unbound (UB)/flow through were analyzed by Western blotting using anti-GST rabbit polyclonal antibody (Sigma #G7781, 1:5000 dilution).
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bioRxiv - Biochemistry 2022Quote: ... The diluted lysate was incubated with antibody overnight at 4 °C with rotation: H3K4me3 (4 × 106 cells, 2.5 μg anti-H3K4me3, Millipore, Cat#07-473, Lot#3394198), H3K36me2 (10 × 106 cells ...
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bioRxiv - Cell Biology 2022Quote: ... transferred to a nitrocellulose membrane, and probed with the following antibodies: anti-FLAG M2-HRP (HRP, horseradish peroxidase) conjugate (Sigma, A8592; dilution 1:10,000), rabbit anti-dynein heavy chain DYNC1H1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... was performed on a 1 in 6 series of sections from sham- or 6-OHDA-lesioned animal as previously described[57] using an anti-TH mouse monoclonal antibody (LNC1 clone, EMD Millipore, Burlingame, MA, USA). Briefly ...
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bioRxiv - Pathology 2022Quote: ... The sections were blocked using 1% BSA for 1 hr and incubated with rabbit anti-hamster ACE2 antibody (Product no. HPA000288, Millipore Sigma, Burlington, MA, USA) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunostaining was performed in cryostat sections (14 µm) using the mouse monoclonal anti FLAG M2 antibody (1:500; Ref F1804; Sigma-Aldrich, St Louis, Missouri) and the anti Sp8 C18 antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were pre-blocked with 5% low-fat dry milk in TBS-T and incubated with a rabbit polyclonal anti-KHSRP antibody (Sigma-Aldrich Cat# HPA034739, RRID:AB_10601582), or a monoclonal anti-c-Myc antibody (D84C12 ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Neuroscience 2019Quote: ... 40 μm sections of PFA-fixed mouse brains were stained with the following antibodies: mouse anti-Gad67 (cat. no.: MAB5406; Millipore; 1:1000; RRID: AB_2278725); chicken anti-GFP (cat ...
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bioRxiv - Cell Biology 2019Quote: ... All mutations were confirmed by Sanger DNA sequencing and expression was confirmed by western blotting with anti-Myc primary antibody (1:2000 dilution; Merck Millipore #06-340, Billerica, MA).
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bioRxiv - Neuroscience 2020Quote: ... in TBST before incubating overnight at 4°C in primary antibody solution (4% NGS in TBST) with rat anti-GFAP (Millipore Cat# 345860-100UG, RRID:AB_211868), rabbit anti-GFAP (1:2000 ...
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bioRxiv - Microbiology 2019Quote: ... Blots were probed with the following antibodies diluted 1:5000 in 1X TBS-T containing 5% nonfat dry milk: rabbit anti-FLAG (Sigma-Aldrich Cat# F7425, RRID:AB_439687), mouse anti-FLAG (Sigma-Aldrich Cat# F1804 ...
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bioRxiv - Microbiology 2020Quote: ... Periplasmic and cytoplasmic fractions were separated electrophoretically using SDS-PAGE gels after which Western blotting was performed according to standard protocols using the following antibodies: anti-PhoA (Millipore MAB1012 diluted 1:2,500), anti-mouse-HRP (Abcam ab6789 diluted 1:5,000) ...
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bioRxiv - Developmental Biology 2020Quote: ... Standard IF staining was carried out by incubation of tissue sections with primary antibodies overnight at 4 °C at the following dilutions: mouse anti-acetylated tubulin (Sigma cat. # T7451; 1:1000), mouse anti-RAB11 (BD Transduction Laboratories cat ...
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bioRxiv - Cell Biology 2021Quote: ... Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-alpha-tubulin (Sigma, T6074, 1:1000 dilution) or anti-UKHC (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... We used following combinations of primary (dilution 1:200) and secondary (dilution 1:250) antibodies: mouse monoclonal anti-acetylated tubulin (Sigma, [6-11B-1], T7451) with Abberior Star 635P goat anti-mouse IgG (H + L ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 hours at room temperature and incubated in the primary antibodies against anti-acetylated α-tubulin (mouse monoclonal, 1 mg/ml, Sigma Cat #T7451, USA), diluted in the blocking solution (2 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were purchased from the following sources: anti-HA rabbit polyclonal antibody (pAb; #NB600-345, Novus Biologicals, Englewood, CO; #F3165, Millipore-Sigma, St. Louis, MO), anti-DDK (M2 Flag ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Developmental Biology 2022Quote: ... at 20°C overnight and then treated with the secondary antibody (AlexaFluor 488-conjugated goat anti-mouse IgG; Sigma-Aldrich; 1:1000 in PBS) at 20°C overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5 μg of sheared chromatin were then used per immunoprecipitation with 1 μg of the following specific histone antibodies: anti-H3K4me3 (Millipore, cat. No. 07-473), anti-H3K4me1 (Diagenode ...
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bioRxiv - Microbiology 2022Quote: ... Neurons were stained with the following primary antibodies overnight at 4°C: mouse monoclonal anti-β-III-Tubulin (Tuj1, 1:1000; Millipore cat. No. MAB5564) and rabbit polyclonal anti-SARS-CoV-2 (COVID-19 ...
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bioRxiv - Microbiology 2022Quote: ... Both NP and endo Hf fractions were collected for quantitation of the residual endo Hf using an anti-MBP mouse antibody (Sigma, catalog no. M6295-.2ML) in ELISA ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Microbiology 2022Quote: ... the culture medium containing S-VSV were pre-treated to neutralize any residual G*-VSVΔG-GFP by incubating with anti-VSV-G antibody (Millipore, Cat #: MABF2337, 1:1,000 dilution) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for at least 20 hours at room temperature with a combination of the following primary antibodies in PBS-TX with 2% NGS or NDS: guinea pig anti-VGLUT1 (1:1000; AB5905, Sigma-Aldrich, St. Louis, USA), guinea pig anti-VGLUT2 (1:20,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2022Quote: ... AGAL-FLAG was detected 24 hours post transfection by Western blot analysis with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Endogenously expressed AGAL and tubulin were detected with rabbit monoclonal anti-galactosidase alpha antibody (used for staining of AGAL in kidney ...
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bioRxiv - Cell Biology 2023Quote: ... WI-38 or LF-IPF cells were stained with FITC-conjugated mouse monoclonal anti-ACTA2 antibodies at 1:200 dilution (Sigma-Aldrich, cat. nr F3777) overnight at 4°C ...
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bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 3% bovine serum albumin in 0.5% Tween-20/Tris-buffered saline (TTBS) for 1 h and incubated with an anti-2,4-dinitrophenylhydrazone (DNP) adducts polyclonal antibody (1:100, EMD Millipore, Billerica, MA, USA, #MAB2223) or HNE polyclonal antibody (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... then salivary glands were incubated for 24 hours at 4°C with the following primary antibodies: anti-pan-PMCA (5F10, MABN1802, Sigma-Aldrich, dilution 1:250), anti-Dlg (4F3 ...
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bioRxiv - Neuroscience 2024Quote: ... Filters were washed three times with TTBS buffer and then incubated for 1 hour with secondary peroxidase-coupled anti-rabbit antibody (1:7000, Millipore, Cat #401393-2 ML). Immunostaining was revealed by enhanced chemiluminescence luminosity (Amersham Pharmacia Biotech ...