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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Brain slices with Cav2-Cre injection at MPC and lENT were immunostained by Rabbit anti-Cre antibody (Millipore Sigma, Cat#69050-3) and Donkey anti-Rabbit secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were subjected to blocking in 5% milk in TBST and incubated overnight with respective primary antibodies - rabbit anti-HA (1:1500, Cat. No. H6908, Sigma-Aldrich, USA), rabbit anti-c-fos (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed with PBS and incubated with secondary antibodies: goat anti-rabbit Alexa Fluor 488 1:500 AP132JA4 (Millipore, Etobicoke, CAN) and goat anti-mouse Alexa 546 1:500 AP192SA6 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) for 4 hours at 4°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... and then stained for 45min at 37°C in a humidified chamber with both DAPI and with FITC-anti-influenza A polyclonal antibody (Millipore, Billerica, MA) primarily effective for binding viral NP ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by incubation in blocking solution and primary antibody against ChAT (Millipore, AB144P, goat anti-ChAT, 1:500 v/v) for five nights at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... sections were blocked for 1 hour in 10% normal goat serum (at room temperature) and probed with an anti-NeuN Alexa Fluor 488 conjugated antibody (1:100; Millipore-Sigma cat. # MAB377X) for 2 hours at room temperature and protected from light ...
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Immunology 2022Quote: ... and then incubated overnight at 4°C with 1:1000 dilution of primary antibody - either anti-p52/p100 (Millipore Sigma Cat#05361) or anti-β-Actin (Cell Signaling Technology Cat#3700) ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Molecular Biology 2019Quote: Recombinant rcCBP2 was run on SDS-PAGE and subjected to western blotting using anti-hexa histidine HRP (horse radish peroxidase) conjugated antibodies (Sigma; 1:2000) to confirm identity of the purified protein.
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bioRxiv - Bioengineering 2019Quote: ... MA) and the secondary antibody used was HRP-conjugated goat polyclonal anti-mouse IgA (1:500; A4789; Sigma Aldrich; St. Louis, MO). HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used at 1:1000 and included: mouse monoclonal anti-β1/β2-adaptins (clone 100/1; Sigma, St. Louis, MO); mouse monoclonal anti-clathrin heavy chain (clone 23 ...
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bioRxiv - Developmental Biology 2019Quote: ... gills were washed 3 ×20 min with phosphate-buffered saline (PBS) and then immunostained with anti-bromodeoxyuridine antibody (BrdU, Sigma-Aldrich, Inc.). Therefore ...
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bioRxiv - Pathology 2019Quote: ... The membranes were then washed in PBS-T and incubated overnight at 4°C in PBS-T containing 5% milk powder and primary antibody: monoclonal anti-GFAP (Sigma-Aldrich, G3893) in dilution 1:400 or monoclonal anti-actin (MP Biomedicals ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... 5 × 106 cell equivalents were analyzed on 10% PAA / SDS gels and sequentially probed with anti-HA antibodies (Sigma, 3F10, 1:3000), anti-Protein A antibodies (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... After incubating overnight in blocking solution containing a primary antibody (polyclonal rabbit anti-tyrosine hydroxylase; 1:1000 dilution; Millipore; catalog no. 657012), sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed in PBS and PBS-TX followed by incubation with the relevant primary antibody (rabbit anti choleratoxin (Sigma-Aldrich UK) or rabbit anti-Mcherry (1:2000 dilution ...
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bioRxiv - Developmental Biology 2019Quote: ... the membranes were washed 3x in TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2019Quote: Immunoprecipitations were set up with 100 µl solubilised chromatin (roughly equivalent to 4 × 106 cells) and 3 µg of an anti-acetylated histone H3 antibody (Millipore, 06-599) in a total of 1 ml Native ChIP Incubation Buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Molecular Biology 2021Quote: The following antibodies were used for Western blotting at the indicated dilution: anti-FLAG-HRP conjugate (Sigma M2 A8592-1MG, 1:5000); anti-MBTD1 (Abcam ab116361 ...
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bioRxiv - Pathology 2021Quote: ... Rabbit polyclonal anti-calnexin (CNX) antibody (Cat# SAB4503258, RRID:AB_10746486) and all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Genetics 2020Quote: ... 3xFLAG-EED expression and deletion of eed deletion were confirmed by western blots probed with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) and genotyped by PCR with primers ...
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bioRxiv - Systems Biology 2020Quote: ... Resolved proteins were transferred to nitrocellulose membranes and detected by standard Western blotting using monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and HRP labelled anti-mouse IgG secondary antibody (Cell Signalling technology ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were permeabilized and non-specific binding sites were blocked before incubation with rabbit anti-TH antibody (1:1000, AB152, Millipore Sigma, USA) for 48h ...
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining.
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining ...
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bioRxiv - Biophysics 2019Quote: FLAG-BLM (40 nM) was conjugated to Quantum Dots (QDs) by first pre-incubating a biotinylated anti-FLAG antibody (Sigma-Aldrich, F9291) with streptavidin QDs (Life Tech. ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-calbindin (1:1000, mouse, Sigma Aldrich, #C9848). After 3 rinses with PBS for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in a phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled in 2x sample buffer for 5 min to elute the proteins and analyzed by SDS-PAGE followed by Western blot using anti c-myc antibody (Sigma-Aldrich, #C3956).
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bioRxiv - Microbiology 2021Quote: ... (80) and α-PilC (1:5000 dilution) (81) antibodies were used together with horseradish-conjugated goat anti-rabbit immunoglobulin G (Sigma-Aldrich) or anti-mouse sheep IgG antibody (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight followed by goat anti-rabbit secondary antibody conjugated with Cy3 for 1 hr at room temperature (1:500, Millipore, Cat. #AP132C). Since the purpose of this staining was to evaluate the presence of extracellular HMGB1 in the tendon ...
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bioRxiv - Cell Biology 2021Quote: ... tissue sections were washed 3 times with PBS and incubated at room temperature for 2 hrs with Cy3-conjugated goat anti-rabbit IgG antibody (1:500, Millipore, Cat. #AP132C).