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Citations for Millipore Sigma :
7801 - 7850 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen, Karolinska Institute, Sweden) or 4 µg/IP anti-rabbit IgG control (Sigma-Aldrich Cat#I5006; RRID: AB_1163659). Two IPs were performed for LXR and pooled prior to DNA purification.
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bioRxiv - Biochemistry 2021Quote: ... Blots were stripped with low pH stripping buffer and then re-probed for total RSK using RSK1/RSK2/RSK3 rabbit monoclonal primary antibody at 1:2500 dilution (CST, Cat. no. 9355S,) or for β-actin (Sigma, Cat. no. A3854, 1:50000). Phospho-site specific signal was normalized with respect to the total RSK or β-actin signal.
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bioRxiv - Cancer Biology 2019Quote: ... polymerase (PARP)-1 polyclonal antibody (1:1000; Epitomics, Burlingame, CA, USA) and a mouse anti-β-actin antibody (1:10000; Sigma-Aldrich, St. Louis, MO, USA), followed by incubation with secondary antibodies and chemiluminescence detection.
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bioRxiv - Molecular Biology 2022Quote: ... THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% FBS and 50 μM β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA). L929 cells were cultured in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were performed and described previously 56 using antibodies specific to pSTAT3, STAT3, STAT1, STAT5 (Cell-Signaling Technology, Danvers, MA, USA) or β-Actin-HRP (Sigma-Aldrich, St. Louis, MO, USA). Blots were imaged in a Bio-Rad ChemiDoc MP System using enhanced chemoluminescence (ECL ...
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bioRxiv - Molecular Biology 2023Quote: ... for 10 minutes followed by washing with 400 μl TB and incubating with 40 μl of β-Casein (Sigma-Aldrich, 10 mg/ml in TB buffer) for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... For AR and FOXA1 ChIP organoids were double fixed (Singh et al., 2019) with 2 mM Di(N-succinimidyl) glutarate (Millipore Signal, Cat #80424-5MG-F) and then 1% formaldehyde ...
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bioRxiv - Cell Biology 2020Quote: ... 3-Isobutyl-1-methylxanthine (IBMX, ≥ 99%), N-acetyl-L-cysteine (NAC, ≥ 99%) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥ 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pregnant mare serum gonadotropin (PMSG ...
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bioRxiv - Physiology 2022Quote: ... and streptomycin (100 μg/ml) and in presence or absence (negative control) of 20 mM sodium lactate (catalog n°L7022, Sigma-Aldrich, Saint-Louis, MO, USA), 1,5 mM sodium pyruvate (catalog n°11360-070 ...
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bioRxiv - Neuroscience 2020Quote: ... SND75 devices and Aclar sheets were coated separately using 100 μg/ml poly L-ornithine (PLO) (Sigma, St. Louis, MO USA, Cat N° P3655-100MG) in DPBS (Cat N° 14190250 ...
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bioRxiv - Microbiology 2021Quote: ... the MDCK monolayers were washed twice to remove FCS using PBS containing 1 μg/mL of trypsin treated with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) (Sigma-Aldrich, St. Louis, Mo, USA), inoculated with each sample ...
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bioRxiv - Physiology 2022Quote: ... and Trpc6E897K/E897K (n=15) mice were given daily injections of low-endotoxin bovine serum albumin (BSA, A-9430, Sigma Chemical Co, St. Louis, MO) in sterile saline (300mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7) gradient (20%, 40%, 60%, 80%, 100%), incubated 3 times (1h, o/n, 2h) in 100% dichloromethane (DCM) (Sigma-Aldrich, St. Louis, MO, USA) to remove hydrophobic lipids ...
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bioRxiv - Microbiology 2020Quote: ... The optimization of the analytical parameters in MS was performed by infusing the internal standard N-heptanoyl-L-homoserine lactone (C7-HSL) (Sigma-Aldrich, Saint Louis, MO, USA).
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Neuroscience 2020Quote: ... fixed with vacuum grease and kept in physiological Tyrode’s solution for imaging (in mM: 120 NaCl, 2.5 KCl, 30 glucose, 2 CaCl2, 1 MgCl2, and 20 HEPES; Sigma Aldrich; pH = 7.2-7.4; n = 1.33).
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Cancer Biology 2022Quote: Western blotting of nuclear extracts or whole cell extracts was performed by standard methods using antibodies specific for the N-terminal portion of ETV6 (Sigma, # HPA000264; Santa Cruz, #sc-166835x), CTCF (Cell Signaling ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... in case of dissolving and removing the remaining oil, 200 μL n-Hexane (Aladdin, catlog no. H140380-1L) with 1% (v/v) span80 (Sigma-Aldrich, catalog no. S6760-250ML) was added to microgels to dissolve any remaining oil ...
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bioRxiv - Microbiology 2022Quote: ... each flask was spiked with 3 ml of 100 mg NH4+-N L-1 NH4Cl or 100 mg Mn2+ L-1 MnCl2·4H2O (Sigma Aldrich, Saint Louis, Missouri USA). Liquid samples (1 ml ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Microbiology 2023Quote: ... Single colony was picked up into 50 ml 3-(N-morpholino) propane sulfonic acid medium (MOPS) buffered defined liquid medium (Sigma-Aldrich, St. Louis, MI, USA). For DSM1092 ...
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bioRxiv - Microbiology 2019Quote: ... All mice were ovariectomized (OVX) and subcutaneously implanted with a silastic capsule containing 17/β-Estradiol (E2, #E8875, Sigma; 50µg in 25µl of 5% ethanol/sesame oil) or Veh (5% ethanol/sesame oil ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were diluted in 0.1 % Triton X-100 in PBS (β - tubulin III: ab78078, abcam, 1:500; MAP2: M4403, Sigma, 1:500; PAX6: 60094, StemCell, 1:500) and incubated on the CO sections in a humidified chamber at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... DPSCs were cultured in the mineralization-inducing fluid (5% FBS, 10 mmol/L β-sodium glycerol phosphate, Sigma; 10−7mol/L dexamethasone, Sigma; 50 ng/mL ascorbic acid, Sigma) containing 0 ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted membranes were blocked with 5% milk in TBST for 30 minutes and probed overnight (4°C) with primary antibody (anti-β-actin [Sigma-Aldrich, A5441], anti-HA [Biolegend, 901501]). After 3 washes with TBST ...
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bioRxiv - Plant Biology 2019Quote: ... water was replaced with assay buffer containing 34 mg/L luminol sodium salt (Sigma, #A4685), 20 mg/L horse radish peroxidase (Fujifilm Wako ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed using ice-cold radioimmunoprecipitation assay buffer containing protease inhibitor cocktail (Sigma, P8340) and phosphatase inhibitor (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink® proximity ligation assays were carried out according to manufacturer’s instructions (Duolink®, Sigma). The average number of PLA dots detected per cell was calculated using DotCount software and data represents the mean of at least two independent experiments ± SD.
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bioRxiv - Developmental Biology 2021Quote: ... The total amount of glucose was determined using the glucose (HK) assay reagent (Sigma, G3293) following a 15 min incubation at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). Further details are described in the S1 Supplemental Methods.
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bioRxiv - Cell Biology 2022Quote: The cAMP enzyme linked immunosorbent assay (EIA, CA-200, Sigma-Aldrich, St. Louis, MO, USA) was used to evaluate the levels of intracellular cAMP levels ...
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bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
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bioRxiv - Immunology 2019Quote: In vitro chemotactic assays were performed in 24-well Millicell hanging-cell culture inserts (Millipore). Briefly ...
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bioRxiv - Neuroscience 2019Quote: Frozen cortexes were homogenized in radioimmunoprecipitation assay (RIPA) buffer containing a protein inhibitor cocktail (Sigma). Samples were centrifuged at 7000 g for 5 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: Fitness costs were assessed by a growth curve assay using M9 minimal medium (Sigma-Aldrich). Rif-R E ...
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bioRxiv - Genomics 2021Quote: ... and TNFα) were determined using a custom bovine cytokines MilliPlex xMAP assay (Merck Millipore, USA). Data were recorded on a MAGPIX flow cytometer using Xponent software (Luminex ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Bioengineering 2020Quote: ... and TNFα.) using a MILLIPLEX® MAP mouse angiogenesis magnetic bead-based assay (EMD Millipore). The assays were read on a calibrated Bio-Plex® 200 System ...
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bioRxiv - Neuroscience 2021Quote: MILLIPLEX® 96-Well Plate Assays were performed according to the manufacturer’s protocol (EMD Millipore). Briefly ...
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bioRxiv - Neuroscience 2021Quote: Proximity ligation assay (PLA) was performed in hippocampal neurons according to manufacturer’s instructions (Duolink, Sigma). Until the PLA probe incubation step ...
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bioRxiv - Genomics 2019Quote: ... 1000 µl assay medium containing 75 mM Tris pH=7.5 (Merck Millipore, Burlington, MA, USA), 7.5 mM MgCl2 (Merck) ...
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bioRxiv - Systems Biology 2021Quote: Cells collected was dissolved in RIPA (Radioimmunoprecipitation assay) buffer (EMD Millipore Cat No-20-188), sonicated in ice for 15s ...
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bioRxiv - Microbiology 2020Quote: For assessing in vivo intestinal permeability fluorescein isothiocyanate (FITC)-labeled dextran assay (4kDa, Sigma Aldrich) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoturbidimetric assay was standardised against purified mouse albumin standards (Sigma Chemical Co. Poole, UK) with samples diluted in phosphate buffer saline as appropriate ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Cancer Biology 2019Quote: Transwell migration assays were performed using 24-well polycarbonate Transwell® inserts (3422, Sigma Aldrich). LNCaP ...
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bioRxiv - Cancer Biology 2021Quote: 2D cell growth assay was analyzed using MTT (Thiazolyl Blue Tetrazolium Bromide (Sigma Aldrich #M2128)) ...
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bioRxiv - Cell Biology 2021Quote: ... In situ proximity ligation assay (PLA) was performed using the Duolink InSitu Reagents (Sigma-Aldrich) according to the manufacturer’s instructions ...