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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and incubated overnight at 4°C with primary antibodies to label cells containing tyrosine hydroxylase (anti-tyrosine hydroxylase monoclonal, 1:1000 Millipore, MAB318) and mCherry (anti-dsRed polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Microbiology 2023Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit IgG and visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Burlington, MA) and an ImageQuant LAS4000 digital imaging system (GE Healthcare ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (Cat#MAB-318, Millipore Sigma) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence (IF): mouse anti-KHC (clone H2; MAB1614; recognizes primarily KIF5C34) (1:100, EMD Millipore); goat anti-KLC1 (V-17 ...
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bioRxiv - Cell Biology 2023Quote: ... each coverslip was washed 3 times in PBS then placed on top of 50 µL drops of the secondary antibody mixture of goat anti-rat TRITC (1:100; Millipore Sigma) and goat anti-mouse FITC (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... BSA in Tris buffered saline and Tween 20 (TBS-T) buffer for 1 h and incubated overnight with the following primary antibodies: Anti-LINE1 ORF1p (MABC1152) (EMD Millipore), Recombinant Anti-LINE1 ORF1p antibody [EPR21844-108] (Abcam) ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of DRM and DSM fractions were analyzed by SDS-PAGE followed by Coomassie staining and Western blotting for detection of IsdF-3xFLAG using an anti-FLAG M2 antibody (Sigma, #F3165) and infrared imaging (Odyssey CLx ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated in PBS supplemented with the pan-flavivirus anti-Env monoclonal antibody 4G2 (NovusBio, dilution 1:1000) at the presence of 0.05% Saponin (Sigma-Aldrich, France) for 1h ...
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bioRxiv - Biochemistry 2023Quote: ... Tubulin was used as the loading control for normalization and probed with Mouse anti-Tubulin antibody (SIGMA, 1 in 3000 dilutions). All the immunoblots of GFP variants were developed ...
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bioRxiv - Biophysics 2023Quote: ... we weakened N-cadherin-mediated cell-cell adhesion of SMCs [49] by using the medium with the addition of mouse monoclonal anti-N-cadherin antibody (10 µg/mL, C3865, Sigma-Aldrich). The SMCs were cultured for 48 h on trough-shaped concavity arrays ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich (A2179)) in PBST ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Genetics 2023Quote: ... The biotin molecules incorporated into the DNA were visualized by indirect immunofluorescent labeling using 1:60 diluted mouse anti-biotin as a primary antibody (Sigma-Aldrich) applied in 1% BSA/1×PBS/0.1% Triton X-100 for 45 min at RT in a humid chamber ...
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bioRxiv - Microbiology 2023Quote: ... Presence or absence of a ZCCHC3 specific band with each clone was determined by western blotting using an anti-human ZCCHC3 antibody (SAB1408147, Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... the chromatin was sheared with a Covaris S220 sonicator to fragments between 300 and 1,000 bp and subsequently and immunoprecipitated with anti-H3K4me3 antibody (Millipore 04-745) or anti-CTCF antibody (Active Motif 91285) ...
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bioRxiv - Neuroscience 2023Quote: ... then labeled with a 1:100 dilution of anti-nucleoprotein monoclonal antibody blend (Light Diagnostics Rabies DFA Reagent, Millipore Sigma 5100) as well as a 1:250 dilution of rabbit anti-Cre polyclonal antibody (Millipore Sigma 69050 ...
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bioRxiv - Developmental Biology 2023Quote: ... from wing imaginal discs of Salm-3xFlag genotype (strain F1049, courtesy of Dr. Frank Schnorrer) using anti-Flag antibodies (Sigma-Aldrich). The samples from anti-Flag immunoprecipitated chromatin and controls were sequenced at the Centre for Genomic Regulation ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBS again and incubated for 48h with a rabbit anti-TH antibody (1:1000, Millipore Cat# AB152, RRID:AB_390204) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... An additional washing step was followed by a 1 h incubation at RT with the primary mouse anti-c-myc antibody (500 µl, 1:200, Sigma Aldrich). Further ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labeled secondary antibody (1:10) (Sigma-Aldrich). Further ...
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bioRxiv - Cancer Biology 2023Quote: ... for at least an hour before incubation with 1 ug/ml primary antibody anti-phospho-histone H2A.X (Ser139) (Millipore, 05-036) and primary anti-53BP1 (Abcam ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hour at room temperature with a horseradish peroxidase conjugated anti-mouse secondary antibody (Cat# NA931-1ML, Millipore Sigma) diluted 1:1000 in 3% milk ...
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bioRxiv - Neuroscience 2023Quote: ... every 4th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with mouse anti-FLAG monoclonal antibody (1:500; MAB3118, Sigma-Aldrich) or mouse anti-HA monoclonal antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... The fixed animals were washed sequentially in Borate-Triton buffer (with added βME) and then incubated at 4°C overnight in mouse anti-acetylated tubulin primary antibody (Ab) (Sigma T6793), at a dilution of 1:100 in 0.5% PBST ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained for 1 hr at room temperature using primary antibody for anti-CDHR5 (1:200; Sigma catalog no. HPA009081), anti-CDHR2 (1:75 ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then incubated overnight at 4°C in primary antibody solutions with anti-α-tubulin (mouse monoclonal, 1:1,000, T9026, Sigma-Aldrich) or anti-TOMM20 (Rabbit polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with homemade anti-RBM20 (1:400) primary antibody in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2022Quote: ... Hxk2-3V5 and associated proteins were immunoprecipitated from glass bead extracts (250 μg total protein) using 20 μL of agarose conjugated anti-V5 antibody (Sigma; A7345). The extract and beads were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA-tagged antibody conjugated with a PLUS oligonucleotide was prepared using the Duolink In Situ Probemaker PLUS (DUO92009; Sigma-Aldrich). The specimens were washed twice with wash buffer A and were subsequently incubated with Duolink ligation solution for 30 min at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... Collected adrenal sections were probed with antibodies for TLQP-21 (rabbit anti-TLQP-21, 1:100, 20) or Tyrosine Hydroxylase (sheep polyclonal TH, 1:100, Millipore Sigma) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% normal goat serum in MAB for 30 min at RT and incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:10000 for oxt, 1:1000 for other genes, Sigma-Aldrich) at 4°C overnight ...