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Citations for Millipore Sigma :
7451 - 7500 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates on coverslips (Laboratory Glassware Marienfeld) coated with poly-L-lysine (Sigma) and laminin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Respiration was measured under basal conditions as well as after injections of 1 µM Oligomycin (Sigma-Aldrich), 0.5 µM FCCP (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×106 cells were plated in a 12-well plate along with 8 µg/ml polybrene (Millipore) and the specific volume of lentivirus depending on the MOI desired ...
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bioRxiv - Physiology 2024Quote: ... cells were seeded on 6-well plates coated with 0.1% gelatin (Sigma Aldrich, Cat. No: G2500-100G) at 1×106 cells per well ...
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bioRxiv - Biophysics 2024Quote: ... The samples are placed in few-mm sized wells that are cast of 2% agarose gel (Sigma) prepared with HM ...
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bioRxiv - Cell Biology 2024Quote: ... dissociated neurons were plated into 24-well plates coated with 100μg/ml poly-D-lysine (Sigma-Aldrich) and 10μg/ml laminin (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... at 400,000 cells per well on double-coated [hESC-qualified Matrigel Matrix and Poly-L-Ornithine (Sigma)] 10 cm tissue-culture treated plates ...
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bioRxiv - Neuroscience 2024Quote: ... Corning 6-well cell culture plates were pre-coated with Poly-L-Ornithine (PLO, Sigma #P4957-50ML) and incubated for 1h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The assay deep-well plate was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated at 37°C anaerobically for 5 h ...
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bioRxiv - Genomics 2024Quote: ... we harvested EBs and moved them to an ultra-low attachment 6-well plate (CLS3471-24EA, Sigma) in E6 media (A1516401 ...
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bioRxiv - Developmental Biology 2024Quote: ... Drug-resistant wells were subsequently processed for single-cell isolation using Accutase (Sigma-Aldrich, catalog no. A6964), diluted ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed and the nuclei stained for 1 h at RT by addition of 33 μl 16% (w / v) para-formaldehyde (PFA)and 4 μg / ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed with phosphate buffer saline (PBS) and then binding buffer and stained with annexin V-FITC and Propidium iodide (50 µg/ml; Sigma-Aldrich, St. Louis, MO) for 15 min ...
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bioRxiv - Bioengineering 2020Quote: Alizarin Red S staining was conducted by applying slices after fixation and washing to the 2 (w/v) % Alizarin Red S staining solution (Sigma Aldrich; pH = 4.1-4.3) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% (v/v) normal horse serum in TBS) for 1 hour at room temperature followed by incubation in rabbit anti-TH (Millipore Cat# AB152, RRID: AB_390204) (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... but with a 10% (w/v) glucose solution containing a yellow dye (1 mg mL−1 Acid Yellow 17; Sigma-Aldrich, St Louis, Missouri). This procedure was repeated during 8 consecutive days with the papers changed every day and blue and yellow dyes on cottons switched between days.
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bioRxiv - Microbiology 2020Quote: ... 500μL of cell suspension was added to 4.5mL of a 78°C mixture of equal parts 1.0% w/v Gelrite® (Sigma-Aldrich, St. Louis, MO, USA) and two-fold concentrated YS medium ...
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bioRxiv - Cell Biology 2022Quote: ... The sample was washed three times with PBS and permeabilized for 15 min with 0.01% (w/v) digitonin (Sigma-Aldrich, St. Louis, MO, USA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... washed in sterile H2O and sown on vertical plates with agar medium (1 % [w/v] sucrose, 2.2 g/l MS salts [Sigma/Aldrich], 0.8 % agarose, [pH 5.7]) in cultivation room under a photoperiod of 16 h light:8 h darkness and 23 °C and light intensity 110 µmol/m2/s.
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bioRxiv - Plant Biology 2021Quote: ... each rat received 0.1 ml of 1% (w/v) carrageenan (injected into the sub-plantar region of the right hind paw subcutaneously) (Sigma Aldrich, # C-1013, Germany). Paw volumes of the right hind paw of each rat were measured by plethysmometer before and 1 ...
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bioRxiv - Immunology 2020Quote: ... Wapl-degron v-Abl pro-B lines were treated with 150 μM Indole-3-acetic acid (auxin analog, IAA, Sigma-Aldrich, #I3750-25G-A) and 2 mg/mL doxycycline (Dox ...
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bioRxiv - Immunology 2020Quote: ... Equal portions of renal cortex from each mouse were finely minced in digest buffer [4.25mg/mL Collagenase V (Sigma-Aldrich St. Louis, Missouri, USA), 6.25mg/mL Collagenase D (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... respectively using either glass micropipettes filled with 0.5 M NaCl solution containing with 1.5% w/v Biocytin (Sigma Aldrich, USA, impedance 3-25 MΩ) or Silicon probes (single shank ...
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bioRxiv - Neuroscience 2022Quote: ... with 690 μl Buffer B (50 mM Tris,pH 8, 10 mM NaCl) that contained 20% w/v glucose (Sigma Aldrich, cat. no. D9559). The buffer was prepared on ice.
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bioRxiv - Systems Biology 2022Quote: Synchronized wildtype adult worms were washed and incubated with 0.005% CFW from a 1% stock (w/v, dissolved in DMSO; Fluorescent Brightener 28, Sigma-Aldrich, CAS 4404-43-7) in 500 µL NGM for 3 hours in the dark ...
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bioRxiv - Cell Biology 2019Quote: hMSCs were harvested with trypLE™ at 60 – 70 % confluence and a solution containing 6.105 cells in 70 μL medium were mixed with a 30 μL 3 % (w/v) liquid low melting agarose solution (i.e. stored at 37 °C) (Sigma-Aldrich, Saint Quentin Fallavier, France) diluted in culture medium containing 50 μg/mL Gentamicin (Sigma-Aldrich ...
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Measuring adaptation dynamics to hydrogen peroxide in single human cells using fluorescent reportersbioRxiv - Cell Biology 2020Quote: ... solution was prepared on ice the day of the experiment by adding 10μL of stock H2O2 solution 30% w/v (#16911, Sigma-Aldrich, L’Isle d’Abeau Chesnes, France) in 10mL sterile mq-water ...
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bioRxiv - Plant Biology 2019Quote: ... were grown at 37°C in Luria-Bertani (LB) medium or tryptic soy agar containing 0.003% (w/v) Congo red dye (Sigma-Aldrich, St. Louis, MO, USA) (Runyen-Janecky and Payne ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... tracheotomized and their lungs were installation-fixed for 5 minutes at 20cm H2O hydrostatic pressure with 4% (w/v) paraformaldehyde (PFA) (Sigma-Aldrich, Oakville, ON, Canada), pH 7.4 ...
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bioRxiv - Physiology 2021Quote: ... a synthetic reduced vitamin D analog which is activated in the liver and is independent of renal hydroxylation (0.4% w/v dihydrotachysterol (Sigma-Aldrich, St. Louis, MO, USA) per 100 g of standard rodent food ...
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bioRxiv - Physiology 2021Quote: ... Parametric and non-parametric tests were used based on results from normality (Shapiro-Wilk) and equal variance (Brown-Forsythe) tests (Sigma Plot v.13, Systat). All data are shown as mean ± SD or scatter plots with bars indicating group means ...
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bioRxiv - Biochemistry 2022Quote: ... The parafilm was washed with water and blocked with 1x phosphate-buffered saline (PBS) containing 3% (w/v) BSA and then incubated in α-HA antibody (Sigma; 1:1.500 dilution) diluted in PBS buffer and 3% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... the growth medium contained 50 mM TMA as the growth substrate and agar solidified HS + TMA media was obtained by adding 1.5% w/v agar (Sigma-Aldrich, St. Louis, MO, USA). To select for transformants ...
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bioRxiv - Cell Biology 2024Quote: ... 0.004% (w/v) bromphenol blue) run on a 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon® -FL PVDF membrane, Sigma-Aldrich, cat. #05317). The membrane was blocked with blocking buffer (0.5% Casein ...
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bioRxiv - Cell Biology 2024Quote: Worms at one day of adulthood were analyzed for the ability to burrow through 26% (w/v) pluronic F-127 (Sigma-Aldrich, St. Louis, MO) gel (Fig.2E) ...
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bioRxiv - Genetics 2023Quote: ... Electrophoresis was run at 120 V for 50 min in 1X tris-glycine-SDS running buffer (25 mM tris base (Sigma; cat. no. T1503-1KG), 190 mM glycine (Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar spinal cord from L1 - L6 was removed and embedded in 2.5% w/v agar (No. A-7002, Sigma-Aldrich, St Louis, MO, USA). The agar block was then superglued with Loctite 401 (Henkel Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... through electrophoresis at 200 V for 40 min and transferred to a polyvinylidene dilfluoride (PVDF) membrane (Immunobilon™ transfer membrane; Millipore, Bedford, MA, USA) at 100 V for 90 mins ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslip has 10μL concentrated ABP-dextran / agarose mixture (1%w/v low gelation temperature agarose, Sigma-Aldrich, with 10mg/mL ABP-dextran in aCSF) pipetted from 42°C heat block immediately before inversion and placement over cortex ...
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bioRxiv - Biochemistry 2024Quote: ... bovine serum albumin (Fraction V Stock, 100 µg/mL), and PureCol (collagen, 30 µg/mL) suspended in α-MEM (each Sigma-Aldrich, MI, USA). 16HBE14o-expansion media was α-MEM (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Guts were incubated overnight at 4°C with a primary anti-catalase antibody (C0979, Sigma; 1:250 in 5% w/v BSA in PBS). After three washes with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... After 48 hours the cells were lysed in RIPA buffer (150 mM Tris-HCl [Sigma-Aldrich], 150 mM NaCl [Sigma-Aldrich], 0.5% (w/v) sodium deoxycholate ...
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bioRxiv - Genomics 2024Quote: DNA indexes with different overhang sequences were validated by performing in-situ ligation assays on 4T1 mouse cells grown on 25mm round coverslips coated in poly-L-lysine overnight (Sigma P4707, 0.01% w/v). Cells were grown for 24-48hrs to ∼90% confluence.
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bioRxiv - Developmental Biology 2024Quote: ... TrypLE Expres Enzyme activity was inactivated with the addition of pre-ice-chilled 200 ul 0.04% (m/v) non-acetylated BSA in DPBS (Sigma-Aldrich: B6917-100MG, Gibco: 14190144). The resulting cell suspension was underlayed with 200 ul of 1% non-acetylated BSA in HBSS (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... or 96 hpf and fixed in groups of 10-15 fish per 1.5 mL Eppendorf® Safe-Lock microcentrifuge tube (Millipore Sigma, Cat. No. T9661-500EA) in 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2019Quote: ... Neurons were plated (12-well plate) on poly-L-lysin-hydrochloride (Sigma-Aldrich, St. Louis, Missouri, United States) coated coverslips in plating medium (500 mL MEM ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
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bioRxiv - Cell Biology 2020Quote: ... as well as rabbit polyclonal antibodies against Phospho-Histone H3 (Ser10) (Millipore, Cat. #06-570, 25 ng/ml), TPX2 (Proteintech ...
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bioRxiv - Immunology 2022Quote: ... All wells were treated for 1 hour with 10 µM 2’,7’-dichlorofluorescin diacetate (DCF-DA, Sigma, D6883) in Ringer buffer (155 mM NaCl ...