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Citations for Millipore Sigma :
7151 - 7200 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 3 μl of the culture was spread on BHIS plates containing 100 ng/ml of anhydrotetracycline (Sigma) for counter selection ...
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bioRxiv - Microbiology 2024Quote: ... washed 3 times with 1x phosphate-buffered saline (PBS) then mounted in Fluoroshield histology mounting medium (Sigma). For each experiment ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were anesthetized with 200 mg/l 3-aminobenzoic acid ethyl ester (MS-222) (Sigma-Aldrich, A5040) and embedded laterally in 1.2% low-melting-point (LMP ...
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bioRxiv - Microbiology 2020Quote: ... The sample was diluted and purified with a 3 kDa Amicon Ultra centrifugal filter (Millipore, Temecula, CA, USA) to remove low molecular weight impurities ...
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bioRxiv - Microbiology 2020Quote: ... plates supplemented with 3% sheep blood (iTek StPaul MN) and 20 μg/mL neomycin (Sigma St Louis MO) and then grown overnight at 37°C in a 5% CO2 humidified incubator ...
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bioRxiv - Biophysics 2020Quote: ... the cells were fixed in 3% formaldehyde solution (37 wt.% in H2O, 252549, Sigma-Aldrich, St. Louis, MO) with 0.1% glutaraldehyde solution (25% in H2O ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell lines were regularly passaged every 3-4 days by dissociation with Trypsin-EDTA 0.25% (Sigma Aldrich). The cell lines were regularly subjected to monthly screening for mycoplasma infection ...
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bioRxiv - Cancer Biology 2021Quote: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (L’Isle-d’Abeau-Chesnes, France). Antibodies against phospho-IκBα Ser32 (#2859) ...
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bioRxiv - Immunology 2021Quote: ... After 3 washes in TBST the blot was revealed using the EC™ Prime Western Blotting System (Sigma). The membrane was imaged using a Chemidoc MP imaging system from BioRad.
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bioRxiv - Cell Biology 2019Quote: ... larvae were washed 3 times with 0.1% PBS-Tween20 and incubated in 0.1% w/v collagenase type 1A (Sigma) in PBS at 37°C for 1.5 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Alive embryos older than 16 ss were additionally mounted with 0.016% ethyl 3-aminobenzoate methanesulfonate salt (Tricaine, Sigma) in the LMA and added to the E3 medium to prevent movement during imaging ...
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bioRxiv - Cell Biology 2020Quote: ... glass-bottomed dishes were first cleaned and activated with 1% v/v (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich) in ethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with 3% BSA in PBS 1X and stained with 5μg/mL Hoechst (Sigma Aldrich). Samples’ images were acquired and analysed by high content fluorescence microscope Operetta® (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: Non- and PUUV-Suo infected Mygla.REC.B cells were exogenously stimulated with TLR-3 ligand polyI:C (10 µg/ml, Sigma Aldrich) or Sendai virus (multiplicity of infection 1 ...
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bioRxiv - Molecular Biology 2021Quote: A2780-DNA329 and OVCAR-3 (ATCC) ovarian cancer cell lines were cultured in RPMI-1640 medium (R8758, Sigma). Ovarian cancer COV362 (ECACC) ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in 1X PBS and incubated for 5 min in 50mM ammonium chloride (Sigma-Aldrich, 254134) before mounting on cavity blades (Marienfeld ...
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bioRxiv - Neuroscience 2021Quote: ... 3 ml of an enzyme cocktail [1 mg/ml collagenase A (#11088793001, Sigma-Aldrich, St. Louis, MO, USA) and 0.1 mg/ml elastase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Prior to training rats were randomly assigned to receive either sham surgery or intracranial bilateral lesions to the region of interest under 2-3% isoflurane anaesthesia with local application of bupivacaine (2mg/kg s.c. at 0.8ml/kg; Sigma) at the incision site ...
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bioRxiv - Neuroscience 2021Quote: ... shShank3 injected mice were randomly infused with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %). The treatment was counterbalanced between trials ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %).
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bioRxiv - Molecular Biology 2021Quote: ... Degradation of AID-tagged CHD4 was induced by the addition of 500 μM 3-Indoleacetic acid (I2886, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Denmark) as well as the BKCa blockers paxilline (3 μmol/L with 0.03% DMSO, Sigma-Aldrich, Darmstadt, Germany) and iberiotoxin (100 nmol/L ...
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bioRxiv - Molecular Biology 2019Quote: ... Expression of constructs was suppressed by addition of 0.5 µM and indole-3-acetic acid (IAA; Sigma, I5148). 2i F1 hybrid 129/Sv:Cast/Eij mESCs cells were cultured for 2 weeks on primary mEFs in 2i ES cell culture media ...
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bioRxiv - Biochemistry 2019Quote: ... the probes were transferred into a 0.1% (v/v) solution of 3-aminopropyltrimethoxysilane (APTES) (Sigma, St. Louis, MO) in toluene for 2 h ...
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bioRxiv - Immunology 2019Quote: ... 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Sigma-Aldrich (Saint Louis, MO, USA) and GeneJuice® Transfection Reagent (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... To create the Tmem98A193P allele Edit-R crRNA (sequence 5’-CCAAUCACUGUCUGCCGCUG-3’) (Dharmacon) was annealed to tracrRNA (Sigma) in IDT Duplex buffer (IDT) ...
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bioRxiv - Bioengineering 2019Quote: ... Chips fabricated with polyester terephthalate (PET) track-etched membranes were assembled using (3-Glycidyloxypropyl) trimethoxysilane (GPTMS; Sigma, 440167) and (3-Aminopropyl ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 x in ice-cold choline-buffer and lysed in 0.5% Triton X-100 (Sigma) in double-distilled water for 20 min at room temperature with gentle agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted nsp7 sample was concentrated with a 3 kDa molecular weight cut-off Centrifuge Filter (Millipore Corporation) and then size-separated by a Superdex S75 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were split into 3 replicates containing 6.6 million cells each and treated with either DMSO (Sigma-Aldrich) or 40nM erlotinib (Selleckchem) ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were then stained at room temperature in 0.05 M Tris-HCl pH 7.6 buffer containing 0.5 mg/ml 3-39 diaminobenzidine (DAB, Sigma Aldrich) and 0.01% hydrogen peroxide.
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed with 1X TBS 3 × 5 minutes followed by treatment with 0.05% 3,3’-Diaminobenzidine (Sigma Aldrich) and 0.01% H2O2/1X TBS for 20 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day ...
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bioRxiv - Biochemistry 2019Quote: ... Isolated protein was then buffer exchanged with 0.5 M Triethyl ammonium bicarbonate (pH 8.5) using a 3 kDa cutoff centrifugal filter (Amicon, Millipore). Protein was then estimated using Bradford’s reagent (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... Dialyzed CTPS2 was then concentration approximately 5-fold using a 3 kDa cut-off centrifugal filter unit (Millipore), flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... cells at 3–4 DIV were incubated with culture medium containg 0–0.8 mM methylmethanesulfonate (MMS, Sigma, 129925) or 0–8 µM etoposide (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Fixed collagen samples were washed 3 times with a solution containing 50 mM PIPES pH 7.4 (Sigma, P6757) kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI ...
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bioRxiv - Plant Biology 2020Quote: ... low-fat dry milk) or anti-GFP (Sigma-Aldrich, dilution 1:1000 in TBS-T with 3% BSA) primary antibody at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Mo and PN were then washed in DMEM and placed in 3 μm Boyden chambers (Merck Millipore, France), at the concentration of 0.5 x 106 cells/chamber in 200 µl DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... washed 3 times with phosphate-buffered saline (PBS) and permeabilized in 0.4% Triton X-100 (T878, Sigma-Aldrich), 1% donkey serum (D9663 ...
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bioRxiv - Physiology 2020Quote: ... Each experimental condition was plated in triplicate and 3 wells were always treated with fulvestrant (ICI182,240) (Sigma Aldrich) in order to measure the basal ERα transcriptional activity ...
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bioRxiv - Cancer Biology 2019Quote: Cells were treated with blebbistatin (20μM, 3 hr) (Selleckchem, S7099), fasudil (5μM, 24 hr) (Selleckchem, S1573), ML7 (20μM, 1 hr) (Sigma, I2764), ionomycin (10μM ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Microbiology 2019Quote: ... Membranes were blocked in 0.05% v/v Tween-3% v/v bovine serum albumin-PBS (Sigma Aldrich, USA) overnight at 4°C and washed twice with 0.05% v/v Tween (Sigma Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... permeabilized in 0.1% v/v Triton X-100 in PBS and blocked in 3% w/v BSA (Sigma) in PBS for 1 hr ...
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bioRxiv - Biophysics 2020Quote: ... Cleaned glass coverslips were then treated during one hour with 3-(trimethoxysilyl)propyl acrylate (Sigma #2530-85-0) to promote a strong adhesion between the hydroxy-PAAm hydrogel and the glass coverslips and finally dried under a nitrogen flow ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Bioengineering 2021Quote: ... then rinsed 3 times with PBS and permeabilized with staining buffer (0.1% Triton X-100/PBS, Sigma, 93443) for 1 hour before immunolabeling.