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Citations for Millipore Sigma :
7251 - 7300 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Genetics 2023Quote: ... Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 µg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were loaded onto a Supelco™ Discovery™ HS F5-3 (15 cm×2.1 mm, 3-μm) column (Sigma-Aldrich Co.) kept at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 8029.3), and 0.5% sodium deoxycholate (AppliChem, A1531) in PBS supplemented with Phosphatase Inhibitor Cocktail 2 and Cocktail 3 (Sigma-Aldrich, P5726, P0044) and Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... EE was gently removed and leaves were submerged in lactophenol trypan blue solution (5 ml of lactic acid, 10 ml of 50% glycerol, 1 mg of trypan blue (Sigma), and 5 ml of phenol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then centrifuged at 300 g for 5 min and resuspended in the plating medium (MEM containing 10% FBS, 1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used in 5 % low-fat milk in TBS-Tween buffer: Puromycin (ms, Millipore, 12D10, 1:10.000), β-actin (ms ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Evolutionary Biology 2021Quote: ... Three more washing steps with TBS were performed before resolving the staining with 5 ml of substrate per membrane: a 30 mg tablet of 4-chloro-1-naphthol (Sigma) dissolved in 10 ml of methanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 μl was dropped into fresh KB broth containing 200 μg mL-1 calcofluor white (Fluorescent Brightener 28, Sigma-Aldrich) and plates were incubated overnight at 28°C ...
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bioRxiv - Immunology 2022Quote: ... 1.000 to 50.000 cells were allowed to adhere for 1 hour at 37°C to coverslips coated with 5 μg/cm2 poly-D-Lysine (Sigma). Cells were then washed in PBS 1x ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally detached from the mask and incubated for 1 hour at RT with a 5 μg/mL solution of fibronectin (Sigma) in NaHCO3 100 mM ...
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bioRxiv - Neuroscience 2021Quote: ... Stained sections were rinsed three times (5 min each) in PBS and incubated in 1:1000 Hoechst solution (33342, Sigma) for 20 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Whole brains were dissected and incubated in blocking solution (5% normal donkey serum and 1% Bovine Serum Albumin (BSA; Sigma) in PBS with 0.1% Triton-X100 (PBS-T) ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed with the previous Tween-20 solution and exposed to Hoechst 33258 (5 μg mL-1, Sigma-Aldrich). Fluorescence images were taken on a fluorescence microscope (Leica DMI 3000B equipped with Nikon-AcT1 software) ...
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bioRxiv - Cell Biology 2022Quote: ... It was incubated for 5-10 minutes in EDTA-containing buffer (500ml RPMI, 20mM Hepes, 1% Penicillin/Streptomycin (Sigma P4333), 12.5μg/ml DTT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were refreshed with RPMI-1640 supplemented with 1X B27 minus insulin and 5 μM IWR-1 (Sigma-Aldrich I0161). Two days later ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 4X for 5 min each in T-Buffer followed by a rinse in TBS for 5 min and then incubated in 1:5000 DAPI (Sigma) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... for 10 min at room temperature followed by 1 h blocking step with PBS containing 0.4% Triton X-100 5% goat serum (all Sigma-Aldrich) before incubation overnight at 4°C with anti-Muc2 (Novus Biologicals #NBP1-31231 ...
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bioRxiv - Immunology 2019Quote: ... saturated in blocking solution (PBS-BSA 5%) for 1 hour and finally incubated with primary Abs: rabbit anti-mouse laminin (Sigma), Alexa fluor 594 Goat anti-mouse IgM (Thermofisher ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5 °C for 5 h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation ...
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bioRxiv - Systems Biology 2019Quote: ... p.o.) or vehicle (5 % DMSO, 30 % cyclodextrin, p.o.) 1 h prior to administration of 100 µg zymosan A (Sigma-Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 2 mM β-mercaptoethanol) supplemented with 1 pill of cOmpleteTM EDTA-free Protease Inhibitor cocktail (Sigma-Aldrich). Cells were then sonicated and the lysate was clarified by centrifugation in a Beckman JA-25.50 rotor at 50000 x g for 1 h at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... Sepals were carefully removed by tweezers and dissected buds were put into capillaries containing medium (½ MS, 5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The capillary was fixed into a metal holder (Zeiss ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were blocked with 5% (w/v) bovine serum albumin in PBS and then incubated with 1:50 anti-HA mouse monoclonal antibody (Sigma) in PBS at room temperature for 2 h ...
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bioRxiv - Physiology 2019Quote: ... Hydrolyzed protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, Porcine 1:250, 85450C, Sigma Aldrich) at 37°C for 20 hours ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Neuroscience 2019Quote: ... The rest of the pipette was filled with the same KCl solution containing 5 μM gramicidin D (1:1000 DMSO dilution, Sigma) and 10 μM Fluorescein (Sigma ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell pellets were thawed and resuspended in 630 μl of Buffer 1 with 5 mM imidazole and supplemented with 70 μl of 10 mg/ml lysozyme (Sigma), 1 μl (250 U ...
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bioRxiv - Microbiology 2021Quote: ... were resolved on a 12.5% SDS gel and puromycin-labelled peptides were detected with anti-puromycin (1:5000 mouse anti-puromycin, clone 12D10; Sigma). Prior to antibody detection ...
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bioRxiv - Bioengineering 2020Quote: ... 5) Skin cleaning with saline and application of polyvinylpyrrolidone (PVP-K90, 1% w/v) (Sigma-Aldrich Inch, St. Louis, Missouri); 6 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were cultured in 5 ml osteogenic media (DMEM, 10% FBS, 1% P/S/F, 50 μg/ml L-ascorbic acid (SIGMA), 100 nM dexamethasone (SIGMA) ...
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... tubes stood at room temperature for 5 min before 48 µL of chloroform:isoamyl alcohol 24:1 was added (Sigma #C0549). Tubes were hand-shaken for 30 sec and left to stand for 2 min before centrifuging at 12,000 x g for 15 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... brain tissue samples of 100 ± 5 mg were lysed in 1 mL of RIPA containing a cocktail of protease inhibitors (Millipore), homogenized with Dounce homogenizer (Dutcher) ...
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bioRxiv - Neuroscience 2021Quote: ... aNPCs were infected at MOI=5 with a lentivirus encoding for a Mili-targeted short hairpin (shMILI, plKO.1, Sigma), or for a short hairpin scramble lentivirus (Control ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4°C overnight with the following primary antibodies in 5% BSA in TBST: puromycin (Sigma, 1:25,000) and vinculin (Cell Signaling Technology ...
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bioRxiv - Physiology 2020Quote: ... sections were blocked in 5% donkey serum for 1h and incubated in goat anti-periostin antibody (1/400, ref AF2955 R&D) diluted in 5% donkey serum in PBS (D9663, Sigma) over-night at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then pre-incubated for 1 h at RT in incubation buffer: 5 % normal donkey serum (EMD Millipore, USA) and 0.2 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were incubated at room temperature for 40 min with a 1:5,000 dilution of monoclonal anti-FLAG-peroxidase antibody in PBST supplemented with 5% milk (Sigma, #A8592). The membranes were subsequently washed another 5 times with PBST ...
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bioRxiv - Plant Biology 2021Quote: ... EE was gently removed and leaves were submerged in lactophenol trypan blue solution (5 ml of lactic acid, 10 ml of 50% glycerol, 1 mg of trypan blue (Sigma), and 5 ml of phenol ...
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bioRxiv - Cell Biology 2021Quote: ... iPSC-CMs were frozen in batches (>5 million cells) in CryoStor media (1 million cells/100 μLs CryoStor; Millipore Sigma) and placed in liquid nitrogen for disease modelling experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were blocked from non-specific interactions for 1 hour in a buffer of tris-buffered saline (TBS) with 5% milk and 0.1% Tween-20 (Sigma-Aldrich) under gentle agitation ...
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bioRxiv - Biochemistry 2022Quote: ... Polar Samples were chromatographically separated by injecting a sample volume of 1 μL into a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 mM, EMD Millipore). The flow rate was set to 150 mL/min ...
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bioRxiv - Immunology 2022Quote: ... Mice were then singly housed for 1 hour and then gavaged with 100 μl of carmine red (5% weight/volume; Sigma) in 1.5% methylcellulose ...
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bioRxiv - Neuroscience 2022Quote: ... 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, and 1 mM MgCl2; unless otherwise stated, all reagents were from Sigma). PFA was prepared in batches ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then permeabilized with 0.1% Triton X-100 for 15 min at RT and blocked with 1% bovine serum albumin and 5% goat serum (Sigma). cells were incubated with primary antibodies Esrrb (PP-H6705-00 ...
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bioRxiv - Neuroscience 2022Quote: ... Tubes stood at room temperature for 5 minutes before 48 μL of chloroform:isoamyl alcohol 24:1 was added (Sigma, C0549). Tubes were hand-shaken for 30 seconds and left to stand for 2 minutes before centrifuging at 12,000 RPM for 15 minutes at 4°C ...