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Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and an extract of total cellular proteins was prepared by harvesting the cells by centrifugation and resuspending cell pellets in 50 mM Tris-HCl (pH 8.0) (200 µL per OD unit) and incubated with 5 µg mL-1 lysostaphin (Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... in PBS for 20 minutes followed by 1 hour of blocking in 5% bovine serum albumin (BSA, Sigma-Aldrich). Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 1% FBS and gluMax along with 5 μM CHIR99021 and 2 μM retinoic acid (R2625, Sigma-Aldrich) for 72 hrs ...
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bioRxiv - Cell Biology 2024Quote: ... cell pellets were resuspended in 400μl lysis buffer (150mM NaCl, 50mM Tris–HCl pH 8.0, 5% Glycerol, 1% digitonin (Sigma, #D141), 1mM MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: Tissue was immediately placed in 1 mL ice-cold lysis buffer containing 5 µl/ml protease inhibitors (Sigma #P8340) and minced using fine scissors before mechanical homogenisation with an ice-cold Teflon-tipped glass dounce homogeniser (10 passes with rotation at 350 rpm) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1×107 OE19 cells were treated with virus (MOI 0.7) and 5 μg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 500 ng/μL puromycin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated in primary antibody (rabbit anti-γH2A.X, Cat#9718, Cell Signalling, 1:1000) containing 5% bovine serum albumin (BSA, Sigma) TBST ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated with a PBS 1X solution containing 5% Horse Serum and 1% gelatin (ref. G7765, Sigma) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... 1% penicillin/streptomycin and ITS supplement (5 μg/ml insulin, 5 μg/ml transferrin, 5 ng/ml sodium selenite) (GenDEPOT, Houston, TX; Sigma-Aldrich, St. Louis, MO) and cell passages 6-9 were utilized for all experiments ...
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bioRxiv - Cancer Biology 2019Quote: ... The membrane was UV-crosslinked for 3 minutes/each side and hybridized with probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) and developed as described62 ...
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bioRxiv - Neuroscience 2019Quote: ... ten times into red fluorescent dye (3 mg Di-I [1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate] per 100 μL acetone; Sigma-Aldrich, St. Louis, MO). The probe was inserted several hundred microns into the cortex at each of the four designated points and left in place for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... the eluate was washed 3 times with PBS in an Amicon Ultra-4 Centrifugal Filter Unit (NMWL 3 kDa, Millipore, UFC800324) and finally concentrated to < 250 µl ...
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bioRxiv - Cancer Biology 2020Quote: ... PRL-3 over expressing construct was made by cloning full length PRL-3 cDNA into p3XFLAG-CMV-14 expression vector (Sigma, E7908)
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bioRxiv - Immunology 2022Quote: ... Cells were exposed to LPS (100 ng/mL) for 3 hr with ethanol or HNE (0.3-3 µM, Millipore Sigma, 393204) in appropriate growth media ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Synthetic Biology 2020Quote: ... indole-3-propionic acid (order no. 57400) and indole-3-butyric acid (order no. i5386) have been obtained from Sigma-Aldrich. All ligands have been dissolved in 50mM Tris / 300mM NaCl pH8 buffer with if necessary 1% dimethyl-sulfoxide (DMSO) ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Physiology 2023Quote: Tissue from the LV of 3 sedentary and 3 exercised mice of each sex was fixed in RNAlaterTM (Millipore Sigma, MFCD03453003), and RNA sequencing was performed by Genewiz from Azenta Life Sciences ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... Slides were incubated for 3 minutes in 3% acetic acid and then for 20 minutes in Alcian blue solution (Sigma Aldrich) at room temperature ...
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bioRxiv - Immunology 2019Quote: ... and placed in 1X PBS containing 5 mM EDTA and 5 mM DTT (Sigma-Aldrich). Tissues from 3-4 pups (per infection group ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ml of schizonts at <5% parasitaemia were lysed using 0.1% saponin/PBS (Sigma) for 10 min (room temperature) ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was quenched by addition of 5 μL 5% (v/v) hydroxylamine (Sigma Aldrich), followed by a 15 minutes incubation step ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-chloro-2’-deoxyuridine (CIdU) and 5-lodo-2’-deoxyuridine (ldU) were obtained from Sigma.
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bioRxiv - Genetics 2021Quote: ... protease inhibitors (5 μM E64, 10 μM Bestatin, 5 μM Pepstatin, 48 hours (Merck Millipore)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... injections were resuspended in a 5% EtOH /5% Kolliphor-EL (Sigma-Aldrich Cat. No. C5135) / 90% water for injections vehicle ...
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bioRxiv - Bioengineering 2023Quote: ... Tendons (n = 5/group/time) were then digested with proteinase-K (5 mg/ml) (Sigma) for 18 hours and stored at -20C until further assays could be performed ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 5-fluorouracil (5-FU) and crystal violet from Sigma-Aldrich (St. Louis, MO, USA). They were dissolved in dimethylsulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-diphenyl-2H-tetrazolium bromide solution (MTT) (Sigma-Aldrich, A2231, 5 mg/mL in PBS) to each well ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µl of the supernatant was incubated with 5 µg of amyloglucosidase (Sigma- Aldrich A1602) with 50 mM Na acetate for 30 minutes at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Transduced cells were selected with antibiotics appropriate for the encoded resistance gene (5 μg/ml blasticidin, MP Biomedicals, Santa Ana, CA, USA; 1 μg/ml puromycin, Sigma Aldrich; 1 mg/ml geneticin (G418), Santa Cruz ...
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bioRxiv - Cancer Biology 2021Quote: ... At 6 to 8 weeks of age seven male and female adult mice received four intraperitoneal injections of tamoxifen (Sigma-Aldrich, T5648-5G, CAS# 10540-29-1) dissolved in corn oil (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: The FA standards used in this study were as follows: cis-6-octadecenoic acid (C18:1 ω-12, petroselinic acid; Sigma-Aldrich, St. Louis, MO, USA, #P8750), cis-9-octadecenoic acid (C18:1 ω-9 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were captured on pre-labeled 1×3 mm glass slides coated with 10% poly-L-lysine (Sigma Aldrich, St. Louis, MO, USA) and maintained at 37° during sectioning ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Neuroscience 2020Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice with 0.1% Triton-X in PBS and blocked with 3% BSA (constituted from powder BSA, Roche Fraction V, sold by Sigma Catalog Number: 10735078001) in PBS for 30 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were incubated for 1 hour and 15 minutes in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (item 03450, Sigma-Aldrich, St. Louis, MO) solution ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA with freshly added 1 mM DTT), then concentrated to 500 μl using Amicon Ultra-4 (4 mL, 3 kDa cut-off) (Millipore, Darmstadt, Germany, UFC800324) and loaded onto a Superdex75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended again in 1x PBS (pH□=□7.4) with 3□mg□mL−1 of urease powder from Canavalia ensiformis (Jack bean) (Sigma-Aldrich cat. no. U4002). When fabricating micromachines with peptides attached ...
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bioRxiv - Plant Biology 2020Quote: ... Then samples were incubated for 1 hour and 15 minutes in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (item 03450, Sigma-Aldrich, St. Louis, MO) solution ...
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bioRxiv - Neuroscience 2019Quote: ... Feeding media include agar paste (US Biological, A0940) containing 10% glucose, 0.5% Tryptone (Becton-Dickinson, 628200) or 3% oleic acid (Sigma-Aldrich, 112-80-1). All behavioral assays were performed under blind conditions.
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bioRxiv - Bioengineering 2022Quote: The HEK-pAGDisplay-RBD stable cells grown to 80% confluency were gently detached by Accutase (1/2 solution in PBS, 3 min, Sigma Aldrich cat. A6964) and washed by PBS with 2 g/L BSA (PBSB) ...
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bioRxiv - Neuroscience 2020Quote: ... These free-floating sections were blocked with 1% normal goat serum for 1 hour in 0.01M phosphate buffered saline (PBS) before incubation with with a mouse α-NeuN (Fox-3) antibody (1:1000; Millipore, Cat#: MAB377 (2018)) and a rabbit α-TDP-43 antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and blocked for 1 h at RT with a serum containing 3% bovine serum albumin (BSA; Sigma−Aldrich, cat. no. A9647), 10% goat serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... blocked in 3% BSA followed by overnight staining with lectin GS-II-Alexa594 and an anti-αSMA-alexa488 antibody (1:150, Sigma, in-house conjugated) in 0.5% triton in PBS ...