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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... for one hour and followed with incubation overnight with following primary antibodies in 5% bovine serum albumin (Puromycin 1:2000 (Millipore), GAPDH 1:1000 (Cell Signaling Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone, Sigma-Aldrich Inc., St. Louis, MO, USA) control ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed by incubation for 5 min at room temperature in 1 mL of lysis buffer (0.864% ammonium chloride (Sigma-Aldrich) diluted in sterile deionized H2O) ...
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bioRxiv - Cell Biology 2022Quote: ... expression was induced on Day 0 by addition of Doxycycline 1 μg/ml with the addition of 5 μM DAPT (Sigma). The next day media was changed to 1% FBS whilst maintaining Doxycycline and notch inhibition ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μL of each sample was injected onto a SeQuant® ZIC®-pHILIC 150 x 2.1 mm analytical column equipped with a 2.1 x 20 mm guard column (both 5 mm particle size; EMD Millipore). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Cell Biology 2023Quote: ... MSK-QRESS-KIT] and 1 uL was injected into a ZIC-pHILIC 150 x 2.1 mm (5 um particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 800µl NP-40 buffer (20mM Tris pH=8, 150-200mM NaCl, 0.5mM EDTA, 1% NP-40, 1mM DTT, 5% glycerol and protease inhibitor cocktail; Sigma-Aldrich). Protease inhibitor cocktail (Bimake ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 300μl extraction buffer (50mM Tris pH=8, 150mM NaCl, 0.5mM EDTA, 1% NP-40, 5%, 1mM DTT, and protease inhibitor cocktail [Sigma-Aldrich]). Cell extracts were sonicated and centrifuged at 4°C for 15 min at 16,000 ×g ...
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bioRxiv - Immunology 2023Quote: ... which was stained after stimulation in with 200 ng/ml of PMA and 1 μg/ml of Ionamycin in the presence of Brefeldin A (5 μg/ml; Sigma) and Golgi stop (0.5 μl/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The pH was adjusted to 7.35 when bubbled with 95% O2/5% CO2 using 1 M KOH (Fluka Analytical, Sigma-Aldrich). After cutting ...
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bioRxiv - Physiology 2023Quote: ... and 5 μL samples of each sample were pipetted onto a 1 μm pore size glass fibre filter (Sigma, APFB04700) on top of a vacuum pump assembly (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... After blocking with 5% BSA the membrane was incubated for 1 h with 50 µg of purified PLG (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed with a free-floating section staining protocol for immunofluorescence using unconjugated primary antibodies (βIV-Tubulin, T7941, Sigma‒Aldrich, dilution 1:400; GFAP, glial fibrillary acidic protein, G-A-5, Sigma‒Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... in an anaerobic chamber (Sheldon Manufacturing) in hemin (5 µg/mL) and L-cysteine (1 mg/mL) supplemented Brain Heart Infusion (Sigma) media (BHIS ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were washed again and anti-FLAG M2 antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk/0.1% PBS-T) was added and incubated for 1hr at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... the acrylate functionalized photodegradable monomer was synthesized by suspending 4-[4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy]butyric acid (0.0166 mol, Sigma-Aldrich) in anhydrous DCM (90 mL) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 1 hour in the culture medium supplemented with 5 μM FAK inhibitor PF-573228 (Sigma-Aldrich) before FRAP experiments.
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Developmental Biology 2023Quote: ... and the slides were incubated for 5 minutes in 100 µl of DAPI solution 1 μg/μl (Sigma-Aldrich, #10236276001) before finishing with three more washes of PBS ...
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bioRxiv - Microbiology 2023Quote: ... Culture volumes equivalent to 5 ml of culture at an OD600 of 1 were filtered through 0.22 µm filters (Millipore, # GVWP02500) via vacuum filtration ...
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bioRxiv - Microbiology 2023Quote: ... The slides were washed atleast 4 times with TTBS and before mounting the cells nuclei were visualized by 5 min counterstaining with wash buffer containing Hoechst dye 33342 (1:10,000 dilution, cat no. B2261, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Reaction mixes were made with 10 μM bovine tubulin (PurSolutions) containing 5% Alexa 568-labeled bovine tubulin and 1 mg/mL BSA (Sigma) in assay buffer ...
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bioRxiv - Molecular Biology 2023Quote: A nucleation mix containing 12.5 μM bovine tubulin (5% AlexaFluor 568-tubulin and the remaining unlabeled) and 1 mg/mL BSA (Sigma: A7906) in cold assay buffer was centrifuged at 80,000 rpm in TLA100 rotor (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and suspended in ice cold 50 µl A-Ri-Buffer (5% FBS, 1% GlutaMAX-I, Invitrogen, 35050-079, 9 µM Y-27632, Millipore, 688000 ...
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bioRxiv - Biophysics 2023Quote: ... product of fusion protein Bre1(591-700) -(GGS)4- Bre1(591-700) -(GGS)5-Rad6(1-172) was cloned into vector pET32-a (Novagen) with N-terminal thioredoxin ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was blocked for 1 h in 5% milk in PBS-T then incubated overnight with an anti-flag antibody (Sigma) or actin antibody (Sigma ...
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bioRxiv - Physiology 2023Quote: Hyperthyroidism was induced at a housing temperature of 30 ± 1°C by treating the mice with 0.5 mg/L 3,3’,5-Triiodo-L-thyronine (#T6397, Sigma Aldrich, Germany) in 0.01% BSA and tap water for 12 days ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each sample was pipetted immediately into 1.5 ml Eppendorf tubes containing 5 ul of 1 x Sigmafast Protease Inhibitor Cocktail (Sigma-Aldrich) on dry ice and subsequently stored at −80 °C until analysis.
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bioRxiv - Neuroscience 2024Quote: ... mice were injected subcutaneously (s.c.) with tamoxifen (dose 100 mg/kg, 1/day for 5 days; Cat# T5648, Sigma-Aldrich). Tamoxifen was administered from a stock solution (20 mg/ml in corn oil ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissues were homogenized with lysis buffer (20 mM Tris-Cl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM dithiothreitol (Sigma, #D9779), 100 µg/ml cycloheximide ...
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked and permeabilized for 1 h at room temperature with 5% horse or donkey serum (Vector or Sigma) and either 0.1% Triton-X100 for iPSCs and neural progenitors ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies used included mouse anti-Integrin alpha 5/CD49e (Snaka51, Catalog Number: MABT201, Sigma-Aldrich, Dilution: 1:500), mouse anti-Integrin alpha V (L230 ...
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bioRxiv - Cell Biology 2024Quote: ... in TNE buffer (50 mM Tris/Cl, 150 mM NaCl, 5 mM EDTA, pH 7.4) containing 1 mM PMSF (Sigma #P7626). Cell debris and unbroken cells were removed by centrifugation at 500 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated with 1:5 diluted Duolink® in Situ PLA Probe anti-rabbit Plus (cat#DUO92002, Sigma-Aldrich) and anti-mouse Minus (cat#DUO92004 ...
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bioRxiv - Neuroscience 2024Quote: ... myelinating cultures were treated for 3 hours at 37°C in a humidified 5% CO2 incubator with nocodazole (final concentration 20 μM; 1.2 μL of 5 mg/mL in DMSO was added per 1 mL media; SML1665; Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... sdAb-expressing transgenic lines were subjected to an additional selection with 5 µg ml-1 hygromycin B (HygB) (Sigma-Aldrich). Induction of the constructs was done by supplementing the medium with 0.5 µg ml-1 tetracycline (Tet ...
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bioRxiv - Immunology 2024Quote: ... NK cells were pretreated with DMSO or 2-DG (0.5 mM, 1 mM, 2 mM, 5 mM; Sigma-Aldrich, USA), BAY 87 (10 μM ...
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bioRxiv - Cell Biology 2024Quote: ... which was diluted 1:5 in DMEM media with a final concentration of 10 μg/mL polybrene (TR-1003-G, Sigma). After 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the substrate of choice was added to the chambers at the following concentrations: 1) 5 mM sodium pyruvate (P8574, Sigma), 2 mM malate (M1000 ...
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bioRxiv - Plant Biology 2019Quote: ... 20 μL/mL Phosphatase Inhibitor Cocktail 3 (Sigma, P0044), 20 μL/mL Phosphatase Inhibitor Cocktail 2 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and Pellet Paint Co-precipitant (Merck Millipore #69049-3). Genomic DNA traces were removed with DNA-free DNase Treatment and Removal Reagents (FisherScientific #AM1906 ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides carrying a 3’ Cy5 fluorophore (purchased from Sigma) were ligated subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), for 1 hour at 4°C on rotation ...
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bioRxiv - Cell Biology 2019Quote: ... 3 μl of 8 mg/ml hexadimethrine bromide (Sigma) was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-γ-adaptin (100/3) from Sigma. Hybridomas producing antibodies against LDL receptor (C7 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated in 3% H2O2 (Sigma-Aldrich, Shanghai, China) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pretreated with 3 μM FTY720 (Sigma-Aldrich) for 2 h ...