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Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 0.1% Tween-20 with 5% w/v nonfat dry milk) and incubated with diluted (1:10.000) rabbit polyclonal antiserum (Sigma-Aldrich) against mCherry overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Viral supernatants after 48- or 72-hour transfection were filtered (0.45 μm) and DLD-1 cells were infected in the presence of 5 μg/mL polybrene (Sigma-Aldrich) for ∼24 hours ...
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bioRxiv - Biophysics 2022Quote: ... Flow cells were first incubated with 20 μL of 1 mg/mL streptavidin in 1xPBS for 5-10 minutes (Sigma). Unbound streptavidin was flushed out with two 50 μL washes of SEC buffer (0.1% DDM ...
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bioRxiv - Biochemistry 2022Quote: ... Around 3 g of cells were suspended 1:5 in 50 mM Mops/KOH pH 7.0 supplemented with 2 mM dithiothreitol (DTT, Sigma-Aldrich) and disrupted by sonication while in an ice bath using a SONOPULS GM200 (Bandelin ...
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bioRxiv - Biophysics 2022Quote: ... Resuspended cells were homogenized in a dounce homogenizer and lysed by addition of 1% Triton X-100 and 5 μl of Benzonase Nuclease (Millipore) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at 37 °C/5% CO2 and washed three times with 50 µl per well of PBS (Sigma). Immortalised human umbilical vein endothelial cells (Ea.Hy 926 cells ...
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bioRxiv - Physiology 2022Quote: ... The rats were lightly anesthetized with isoflurane and 5 µl of pyrogen-free 0.9% saline or PGE2 (1 mg/ml dissolved in saline; P5640, Sigma-Aldrich) was injected into the lateral ventricle through the cannula as mentioned above ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl drops of the cultures were added to LB agar blocks containing 1 µM Propidium Iodide (PI, Sigma, Germany) and the covered bacterial area of the blocks were placed on a circular bottom glass open cultivation system II (PeCon ...
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 17 mM sodium citrate, 5 mM EDTA, 0.5 mM DTT, and protease and phosphatase inhibitor cocktail [1:100]; Sigma-Aldrich) for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated for 8 h followed by treatment with 5 μM EPFL6 for 1 h in the presence of 2 μM MG132 (Sigma). The ubiquitinated ERECTA was detected with an α-HA (ab18181 ...
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bioRxiv - Plant Biology 2022Quote: ... The reproductive organs were exposed by removing the sepals and placed into glass capillaries (size 4, Zeiss) containing ½ MS medium (5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The inflorescence embedded in solidified MS was pushed out of the capillary and then placed into the capillary holder for the Z.1 ZEISS light-sheet microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... Working stocks of glycan-biosynthesis inhibitors were all made in DMSO at the following concentrations and stored at -80°C: 5 mM NGI-1 (Sigma), 10 mM Kifunensine (Kif ...
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bioRxiv - Neuroscience 2023Quote: Refractive index matching was achieved by incubating the cleared brains in CUBIC-R+(M) (45 wt% 2,3-dimethyl-1-phenyl-5-pyrazole [Antipyrine; #10784, Sigma-Aldrich, Germany] ...
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bioRxiv - Neuroscience 2022Quote: For differentiation of OPC to OL: OPC medium was supplemented with 40 ng ml−1 3,3ʹ,5-Triiodo-L-thyronine (T3) (Sigma; T6397)52 ...
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bioRxiv - Microbiology 2023Quote: ... a HCCA matrix solution was prepared at 5 mg.mL−1 in 80/20 ACN/water doped with 0.1% TFA (Sigma-Aldrich, Belgium). Then two times 10 layers of matrix were sprayed onto the slides with the SunCollect instrument (SunChrom ...
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bioRxiv - Neuroscience 2023Quote: ... samples were reconstituted in 50 uL water and 2 uL were injected onto a SeQuant® ZIC®-pHILIC 150 x 2.1 mm analytical column equipped with a 2.1 x 20 mm guard column (both 5 mm particle size; Millipore-Sigma). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Microbiology 2022Quote: ... permeabilized for 5 minutes with 0.1% Triton X-100 and blocked for 30 minutes with 5% bovine serum albumin (BSA) (all reagents purchased in Sigma). Cells from infectivity and replications assays were incubated 1 hour at room temperature with anti-cytosolic tryparedoxin peroxidase (cTXNPx ...
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bioRxiv - Biochemistry 2022Quote: ... and phosphatases (1.15 mM sodium molybdate, 4 mM sodium tartrate dihydrate, 1 mM freshly prepared sodium orthovanadate, 5 mM glycerophosphate, all Sigma), and 100 mM N-Ethylmaleimide (NEM ...
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bioRxiv - Immunology 2022Quote: ... Mice were culled 24 hours later and the air pouch flushed with 5 ml of flow cytometry buffer (PBS containing 1 mM EDTA (Sigma) and 1% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Biophysics 2022Quote: ... 5% v/v glycerol and 1 mM TCEP and then concentrated using 10 kDa MWCO Amicon Centrifugal Filters (Millipore Sigma) according to manufacturer’s directions ...
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bioRxiv - Plant Biology 2024Quote: ... v) dilution in PBST (1× PBS, nonfat dry milk [5%, w/v], and Tween 20 [0.1%, v/v; Sigma-Aldrich]). The JMJ27-GFP fusion protein was detected using the anti-GFP (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 µl of the reaction mix were added to 95 µl 5 mM Iodonitrotetrazolium chloride which is a cheaper alternative to WST-1 (INT, Sigma) and 5 µg/ml mPMS in DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... Disulfide bonds were reduced and alkylated by incubation for 1 h at 37 °C in 5 mM DTT (Sigma Aldrich) with subsequent incubation in 15 mM iodoacetamide for 30 min in the dark at room temperature (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in MACS buffer (1×PBS, 0.5%BSA and 2mM EDTA) and blocked with 5% goat serum (Sigma; NS02L) in MACS buffer at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were reconstituted in 50 uL water and 2 uL were injected onto a SeQuant® ZIC®-pHILIC 150 x 2.1 mm analytical column equipped with a 2.1 x 20 mm guard column (both 5 mm particle size; EMD Millipore). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Neuroscience 2024Quote: ... On the next day the slides were incubated with PLUS and MINUS PLA probes (1:5; Sigma; DUO92001 and DUO92005) for 1 h at 37°C followed by ligation with 1X Ligation Buffer (1:40 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... media was changed once per week with the addition of 1 μM 5-Fluoro-2′-deoxyuridine (Cat.#F0503, Sigma-Aldrich) and 1 μM Uridine (Cat.#U3003 ...
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bioRxiv - Biochemistry 2023Quote: ... Polar Samples were chromatographically separated by injecting a sample volume of 1 µL into a SeQuant ZIC-pHILIC Polymeric column (2.1 x 150 mm 5 mM, EMD Millipore). The flow rate was set to 150 mL/min ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.15 M KCl TRAP wash buffer (10 mM HEPES-KOH at pH 7.4, 5 mM MgCl2, 150 mM KCl, 1% NP-40, supplemented with 100 µg/mL cycloheximide [Millipore Sigma C7698-1g] in methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl sample were injected onto a SeQuant® ZIC®-pHILIC 150 x 2.1 mm analytical column equipped with a 2.1 x 20 mm guard column (both 5 mm particle size; EMD Millipore). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of excystation mixture (taken 30 min after induction) was loaded onto a 1 ml syringe and filtered with a 5 μm disposable filter Millex-SV (Millipore) to remove residual non-excysted and empty oocysts ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were washed in MACS buffer (1× PBS, 0.5%BSA, and 2mM EDTA) and blocked with 5% goat serum (Sigma; NS02L) in MACS buffer at 4°C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 5% milk-PBST). Anti-ATS and anti-NapL were detected as previously described (Nacer et al ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were chromatographically separated by injecting a sample volume of 1 μL into a SeQuant ZIC-pHILIC Polymeric column (150 x 2.1 mm 5 µM, EMD Millipore). The flow rate was set to 150 µL/min ...
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bioRxiv - Cell Biology 2023Quote: ... After nanobody staining cells were washed 3 times for 5 minutes with 150 µl PBS and then incubated for 5 minutes with 100 µl of DAPI 1:1000 in PBS for nuclear staining (DAPI from Sigma). The last 3 washes were then performed and cells were imaged directly at the 96-well plate with an inverted DMi8 microscope (Leica) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μL of each sample was injected onto a SeQuant® ZIC®-pHILIC 150 x 2.1 mm analytical column equipped with a 2.1 x 20 mm guard column (both 5 mm particle size; EMD Millipore). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Plant Biology 2022Quote: ... The quantification was conducted by means of standard curves (1, 2.5, 5, 10, 25, 50, 75, 100, 200 and 500 ng/mL of ABA, JA and SA, Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... EVs were obtained by incubating red blood cell with CaCl (5 mM) and other cell types with the calcium ionophore A23187 (1 mM, C9275, Sigma) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... BSA for 15 min at room temperature before incubating with primary anti-Flag antibody (diluted with PBS containing 5% BSA at a ratio of 1:300, Sigma) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and stained with a 1:5000 dilution of DAPI in PBS for 5-10 minutes at RT (DAPI was purchased from SIGMA). Alternatively ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were then isolated with CD14+ microbeads (MACS Miltenyi) and incubated for 5 days in RPMI/10% fetal calf serum medium and 1% penicillin/streptomycin solution (Sigma) supplemented with 25 ng/ml human macrophages colony-stimulating factor (M-CSF ...
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bioRxiv - Biochemistry 2023Quote: ... Afterwards, membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...