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Citations for Millipore Sigma :
7101 - 7138 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... buffer the membranes were incubated for 1 hour at room temperature with anti-rabbit/mouse/goat IgG secondary antibody conjugated with horseradish peroxidase (1:5000; Sigma–Aldrich, St Louis, MO, USA). Signals was detected with Clarity enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2023Quote: ... used in this study were previously cultivated in a rabbit kidney cell lineage (RK-13, ATCC CCL-37) in RPMI medium supplemented with 10% fetal calf serum (FCS-Sigma-Aldrich, St. Louis, MO, USA) and gentamicin at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.4) for 1 h at room temperature and were subsequently incubated with primary antibodies rabbit anti-claudin-5 (Sigma-Aldrich, cat# SAB4502981, AB_10753223, 1:300), mouse anti-claudin-5 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit α-MORN1 (kindly provided by Dr. Marc-Jan Gubbels, Boston College, MA; 1:100) and mouse α-Centrin1 (clone 20H5; Millipore Sigma; 1:100). Stained gels were washed three times with PBST (1xPBS + 0.1% Tween20 ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 ng of F(ab’)2-Rabbit anti-Rabbit IgG (H+L) (CST, 4412S, Lane Danvers, MA, USA) was diluted in 0.1% Triton X-100 (Sigma-Aldrich, T8787, St. Louis, MO, USA) and incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Neuroscience 2023Quote: ... First antibody (Guinea pig anti-Fos, 1:500 dilution, Synaptic Systems, Cat. # 226005; Rabbit anti-Esr1, 1:3000 dilution, Millipore, Cat. # 06-935, Lot # 3243424) was diluted in PBST with 3% normal donkey serum and incubated overnight (12-16 hours ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% [v/v] Triton X–100) and incubated at 37°C for 3 h either with α–mouse plus and α–rabbit minus for PLA assay (Sigma–Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Bioengineering 2023Quote: ... In a subset of IHC experiments we performed co-immunostaning with additional cell type marker antibodies including the following: rabbit anti-GLUT1 (Millipore catalog #07-1401, 1:1000), rabbit anti-Olig2 (Abcam catalog #AB9610 ...
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bioRxiv - Systems Biology 2023Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signalling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA) for 1 hour at 24°C with agitation ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp., Darmstadt, Germany; rabbit polyclonal antibody, Cat. # C6219, Millipore Sigma, St. Louis, MO), N-cadherin (N-cad ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301, Goat α-mouse HRP 1:1000– Sigma Aldrich/GERPN4401), revealed using ECL reagent (BioRad ...
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bioRxiv - Systems Biology 2023Quote: ... well-validated custom and commercial primary antibodies against proteins of interest were carefully selected and experimental parameters rigorously optimized to ensure high fidelity labeling: Connexin 43 (Cx43; mouse monoclonal antibody, Cat. # MAB3067, EMD Millipore Corp., Darmstadt, Germany; rabbit polyclonal antibody, Cat. # C6219, Millipore Sigma, St. Louis, MO), N-cadherin (N-cad ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 5% skim milk for 1 hour and incubated at 4 °C overnight with antibodies including α-c-Myc produced in rabbit (Sigma-Aldrich, C3956, 1:2,000), and α-HA high affinity produced in rat (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... rabbit anti-NDC80 (dilution 1:1000) (Oliveira et al. 2024) and mouse anti αtubulin (dilution 1:100, Sigma-Aldrich, St. Louis, MO; catalog number T6199). Antibodies against KNL1 and NDC80 were originally developed using respective peptides EDHFFGPVSPSFIRPGRLSDC and EQGINARDAERMKRELQALEG from Cuscuta sp ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies followed by anti-mouse (#A4416) and anti-rabbit (#A0545) secondary antibodies conjugated to horseradish peroxidase (both are from Millipore-Sigma and used at 1:10,000). WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (goat anti-cholineacetyl transferase [ChAT; 1:100; Millipore, Burlington, MA, USA], or rabbit anti-tryptophan hydroxylase [TPH; 1:500; Sigma-Aldrich, St Louis, MO, USA] antibody) were added and tissue was incubated overnight at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated for 24 h at 25°C on a rotary shaker with rabbit anti-NG2 chondroitin sulfate proteoglycan (Millipore, cat no. AB5320, 1:200 dilution) and FITC-isolectin B4 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 3h at 4°C under rotating with 10 µg of primary antibody (Supplementary Table S4; anti-mouse and anti-rabbit whole IgG (Millipore 12-371 and 12-370 respectively)) ...
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bioRxiv - Microbiology 2020Quote: ... 2 hours), α-SodA (1:5000, 30 minutes), α-VSV-G (1:3000, 1.5 hours) and a goat α-rabbit secondary antibody (Sigma, 1:5000, 45 minutes). Clarity Max Western ECL substrate (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen, Karolinska Institute, Sweden) or 4 µg/IP anti-rabbit IgG control (Sigma-Aldrich Cat#I5006; RRID: AB_1163659). Two IPs were performed for LXR and pooled prior to DNA purification.
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bioRxiv - Biochemistry 2021Quote: ... Blots were stripped with low pH stripping buffer and then re-probed for total RSK using RSK1/RSK2/RSK3 rabbit monoclonal primary antibody at 1:2500 dilution (CST, Cat. no. 9355S,) or for β-actin (Sigma, Cat. no. A3854, 1:50000). Phospho-site specific signal was normalized with respect to the total RSK or β-actin signal.
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bioRxiv - Neuroscience 2022Quote: ... rabbit polyclonal anti-CGRP (cat.# T-4032; Peninsula, San Carlos, CA, 1:1000 in diluting buffer, rabbit polyclonal anti-NK1R (cat.#S8305; Sigma-Aldrich; 1:1000 in diluting buffer). Slices were washed in diluting buffer and then incubated in secondary antibody (goat anti-rabbit 647 (cat ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 ℃ in an antibody diluent (1.1% Triton-X and 2% normal goat serum in PBS) containing primary antibodies anti-TUBB3 (mouse, 1:1000, Sigma-Aldrich, T8660; rabbit, 1:300, Abcam, ab18207), anti-SOX2 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers, MA, USA; sheep anti-αMSH, 1:1000, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal anti-mouse IBA1 (Wako ref. 019-19741, RRID AB_839504, 1:1500) and rabbit polyclonal anti-mouse NF200 (Sigma-Aldrich ref. N4142, RRID AB_477272, 1:500). The following day slices were washed with PBS (2×10 min ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin, mouse, Sigma-Aldrich A2228 at 1:2000) was incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-SEC31A (Cell Signaling Technologies, E1J5M, Lot #0, 1:800 for IF and 1:1000 in 5% Bovine Serum Albumin (Sigma, Cat no. A9418-50G) for WB) ...
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bioRxiv - Cell Biology 2020Quote: ... were subjected to immunoblot analysis with specific primary antibodies (Table 1) and secondary antibodies (anti-mouse/rabbit IgG coupled to horseradish peroxidase; Sigma Aldrich; 1:5000 dilution in blocking buffer). Detection of chemi-luminescent signal was performed with Clarity ECL Western Blotting substrate (BioRad ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... for 1h at room temperature before incubation in primary antibodies (rabbit anti-Synapsin I, ab64581, Abcam; guinea pig anti-GLT-1, ab1783, EMD Millipore; mouse anti-FLAG, F1804, Sigma-Aldrich) at 1:1000 in block for 48h ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked and incubated with primary antibodies rabbit polyclonal anti-Rab35 (Antibody Facility MPI-CBG Dresden, F18256, 1:1000) and mouse monoclonal anti-γ-tubulin (Sigma-Aldrich, Cat. No. T6557, 1:2000) and secondary HPR-conjugated antibodies (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed for 10 minutes thrice with 1×TBST before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA) for 1 hour at room temperature with agitation ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were incubated during 48 h at 4°C in a blocking solution containing the primary antibody against TH (rabbit anti-TH, Millipore AB152, lot 2795024 and 3114503 (1:1500) RRID ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Molecular Biology 2021Quote: ... or GAPDH (mouse monoclonal anti-GAPDH antibody, 1:5000, Thermo Fisher Scientific Cat# AM4300, for fibroblasts and rabbit monoclonal Sigma-Aldrich Cat# G9545, 1:10000 for LX2 cells). Appropriate HRP-conjugated secondary antibodies were used (1:10000 Promega) ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000, Sigma-Aldrich; in 5% milk, 1xTBS,0,1% Tween) and washed 3 times with 1xTBS/0.1% Tween ...
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bioRxiv - Cell Biology 2022Quote: ... incubated at room temperature with secondary antibodies coupled to horseradish peroxidase (1:20,000 Thermo Fisher goat-anti-rabbit, or 1:20,000 Millipore Sigma goat-anti-rat in 5% nonfat dry milk). Membranes were washed again in PBS containing 0.1% Tween 4 x 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and incubated with 1x PLA Plus and Minus probes (Duolink® In Situ PLA® Probe Anti-Rabbit PLUS and Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Sigma-Aldrich, DUO-92002 and DUO92004) were added to each well and incubated 37C for 1h ...