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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Slides were washed 5 times with PBS and blocked with blocking buffer (PBS + 1% bovine serum albumin (BSA, Sigma, cat no ...
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bioRxiv - Developmental Biology 2019Quote: ... the samples were incubated with the blocking buffer (0.1% Triton X-100, 5% normal donkey serum (NDS, Stratech) and 1% bovine serum albumin (BSA, Sigma)) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM EGTA, 10% glycerol, 50 mM NaF, 5 mM NaPPi, 1 mM PMSF, 0.5 mM DTT, 200 mM Na3VO4, Sigma protease and phosphatase inhibitor solutions) ...
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bioRxiv - Biochemistry 2021Quote: ... were coated with 10 mM DOPA-Tet and incubated for 5 days at 37 °C in 1 mL of either 50 mM MES pH 4.5 (Millipore-Sigma), 100 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The Ape1-thrombin mixture was incubated with p-aminobenzamidine-agarose beads (5 mg thrombin:1 mL beads ratio; Sigma) at 4 °C for 15 min to remove thrombin and was then filtered through a 0.22 μm PVDF membrane ...
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bioRxiv - Microbiology 2021Quote: ... D-glucose (5 mg.ml−1) or nothing (no-amendment control) (all amendment chemicals were from Sigma–Aldrich, except D(+)-galactose which was purchased from Carl Roth GmbH) ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated with PBS 1× supplemented with 5% rabbit serum (cat. SCBD33ISV - Sigma Aldrich, St. Louis, MO, USA) to reduce non-specific reactions ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 37 °C and with proteinase K buffer (50 mM Tris-HCl pH 8.0, 5% glycerol, 2.5 mM CaCl2, 1 mg/ml proteinase K (Sigma P2308)) for 30 min at 50 °C ...
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bioRxiv - Microbiology 2020Quote: Total intracellular and extracellular ROS was measured by luminol (5-amino-2,3-dihydro-1,4-phthalazinedione; 1 mM; Sigma-Aldrich) enhanced chemiluminescence as previously described [14] ...
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bioRxiv - Immunology 2020Quote: ... A 5 μm section was deparaffinized and stained with anti-MAP2 antibody (Sigma-Aldrich, 1:1000 dilution, Cat# M4403) (the details of this procedure are described elsewhere (Shichita et al ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Odyssey blocking buffer TBS (Licor) or 5% non-fat milk or 1% BSA (Sigma, A7906) for 1 hour before over-night incubation at 4°C with primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... at 1×106 cells/ml followed by 90 min incubation with Hoechst 33342 (5 μg/ml, Bisbenzimide, Ho342; Sigma) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The MisKO pre and batch cultures were conducted in YPD with 5 mmol L-1 hypoxanthine (Sigma-Aldrich GmbH) and 100 mg L-1 nourseothricin ...
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bioRxiv - Neuroscience 2022Quote: ... in ice-cold homogenization buffer (5 mM HEPES pH 7.4, 1 mM MgCl2, 0.5 mM CaCl2, supplemented with phosphatase (Sigma 4906837001) and protease inhibitors (Sigma 4693159001)) ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
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bioRxiv - Microbiology 2022Quote: ... followed by 4x 8 min in PBS and then incubated in PBS-5% dried semi-skimmed milk with 1:10,000 anti-mouse HRP-linked secondary antibody (A9044, Sigma) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 105 to 1 × 106 cells/ml were resuspended in freezing media (FBS + 10% Dimethyl Sulfoxide, DMSO, Sigma, D2660) and frozen down.
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bioRxiv - Genetics 2022Quote: ... Coverslips were washed twice with 1x PBS for 5 min followed by 1 min incubation in permeabilisation buffer PBT (1x PBS, 0.5% Triton x100 (Sigma)) ...
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bioRxiv - Pathology 2022Quote: ... Slides were then washed in tap water followed by a 5-minute incubation in 1% periodic acid (Sigma; 375810). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in Cell Block (1X PBS with 1% BSA and 5% normal goat serum) with 0.4% Saponin (Sigma) for one hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...
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bioRxiv - Neuroscience 2022Quote: ... mixed glial cultures were washed in Dulbecco’s modified Eagle’s medium (DMEM) before incubation in a 5:1 mix of DMEM and trypsin-EDTA (Sigma #T4174). After detachment of astrocytes ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets from a total of ∼5ODs were resuspended in 400μl lysis buffer (150 mM NaCl, 50mM Tris-HCl pH 8.0, 5% Glycerol, 1% digitonin (Sigma, #D141), 1mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... testes were incubated in medium containing 1 mM 5-ethynyl uridine (EU) for 20 min at 25°C and fixed with 3.7% Formaldehyde (Sigma), 1 % Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and an extract of total cellular proteins was prepared by harvesting the cells by centrifugation and resuspending cell pellets in 50 mM Tris-HCl (pH 8.0) (200 µL per OD unit) and incubated with 5 µg mL-1 lysostaphin (Sigma) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Next the membrane was blocked with PBS-milk 5% and incubated with primary antibodies s9.6 1:500 (Millipore, MABE1095) and dsDNA 1:3000 (Abcam ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Microbiology 2024Quote: ... Blots were blocked in 5% skim milk (Carnation) in Tris-buffered saline (TBS)/Tween (TBS + 1% Tween 20; Sigma) overnight at 4 °C with gentle shaking ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 1 hr with 5 mg/mL collagenase type I (Sigma, cat#C0130-500MG, St Louis, MO) diluted in HBSS supplemented with 0.1 ug/mL TLCK trypsin inhibitor (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 1 hr in TBST (Tris-buffered saline with 0.1 % Tween 20) supplemented with 5 % bovine serum albumin (Sigma) and then incubated overnight at 4 °C with anti-Kv1.1 primary antibody (Rb-Af400 ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 1 ml lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40, protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... Membrane was washed 3 times for 5 minutes with TBS-T and incubated in secondary antibody 1:10,000 dilution goat anti-mouse HRP (Sigma).
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... ADP 15N5 at 0.1 µg/mL (dissolved at 1 mg/mL in 5 mM Tris-HCl, Sigma-Aldrich, 741167), and EquiSPLASH™ LIPIDOMIX at 0.02 µg/mL (prepared as 100 µg/mL ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... ATP 13C10 at 0.1 µg/mL (dissolved at 1 mg/mL in 5 mM Tris-HCl, Sigma-Aldrich, 710695), AMP 13C10 ...
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bioRxiv - Microbiology 2023Quote: ... Amplified DNA was analyzed by electrophoresis in 1.5% agarose gel in 0.5 x TBE buffer stained with 5 mg mL-1 of ethidium bromide (Sigma-Aldrich). Gels were developed for 6-7 h at 100 V at room temperature (approx ...
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bioRxiv - Microbiology 2023Quote: ... and an extract of total cellular proteins was prepared by harvesting the cells by centrifugation and resuspending cell pellets in 50 mM Tris-HCl (pH 8.0) (200 µl per OD unit) and incubating with 5 µg ml-1 lysostaphin (Sigma) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA fibers were then denatured in 2.5 M HCl solution for 1 hr at room temperature and blocked with 5% bovine serum albumin (BSA; Sigma) in 1xPBS for 45 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
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Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disordersbioRxiv - Molecular Biology 2023Quote: ... Cells were maintained at 37 °C in the presence of 5% CO2 and incubated in high-glucose Dulbecco’s modified Eagle medium supplemented with 1% penicillin/streptomycin (Sigma), 15% fetal calf serum (heat inactivated ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with 5% normal donkey serum for 1h at room temperature and incubated with Nissl (1:2000, in 0.1M PB, Sigma) for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 5 % w/v milk powder for 1 h at room temperature after staining in Ponceau-S (Sigma Aldrich). Incubation by primary antibodies (Supplementary Tab ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Cell Biology 2023Quote: ... was followed by 5 % milk block as well as primary antibodies FLAG-M2-Peroxidase (1:2500, Sigma-Aldrich, USA), TULP3 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were then probed overnight at 4°C with the following antibodies diluted in 5% BSA-TBST: Mouse anti-FLAG monoclonal antibody (1:10,000 dilution, Cat #F1804, Clone M2, RRID: AB_262044, Sigma Millipore), Mouse anti-Menin monoclonal antibody (1:2,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-Sox4 protein was eluted by 5 rounds of competitive elution with 1× column volume (0.6 ml) of 100 μg/ml FLAG peptide (Sigma) in TBS ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were rinsed three times with PBS and blocked for 1 h in 5% BSA (w/v) (Millipore, 126579) in 0.2% Triton-X100/PBS (blocking buffer) ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...