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Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with PBS and DNA content were visualized by DAPI (1 μM; Cat# D9542, Millipore Sigma, I h) staining at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were amputated to induce Erk activation and then dissociated at 3 hpa in CMF supplemented with a 1:10 dilution of Liberase TH (Millipore Sigma, 5401135001) into single cells (Witchley et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours post-knockdown and induction coverslips were fixed in -20oC methanol for 3 minutes and stained for ms-gamma tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig CENP-C 1:25 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... Cover glasses were then activated by silanization with an ethanol solution supplemented with 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and stained for 15 minutes in Alcian Blue solution (1% Alcian Blue in 3% solution of acetic acid) (Cat. # A5268, Sigma Aldrich, UK). Slides were then washed in running tap water for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 1xPBS + 0.5% Tritton X-100 + 3% Bovine Serum Albumin before adding the primary antibody (HPA036814, Sigma-Aldrich – 1/1000) for 48h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted to a starting OD600 of 0.01 in 3 mL of BHIS supplemented with increasing concentrations of 1) bile salt mixture (50% cholate and 50% deoxycholate; Sigma-Aldrich B8756), 2 ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G ...
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bioRxiv - Plant Biology 2024Quote: ... and combined with 0.2 mL of a 3 mg L-1 TAG C15:0 solution (Tripentadecanoin, reference T4257, Sigma-Aldrich™, USA) in anhydrous hexane 95 % (296090 ...
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bioRxiv - Cancer Biology 2021Quote: Organoids were washed once with and collected in ice-cold PBS, supplemented with 5 mM NaF (Vwr, #1.06449.0350) and 1 mM NaVO3 (Sigma, #S6508). Organoids were centrifuged and pellets were incubated in Cell Recovery Solution ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% skim milk and probed with primary antibodies: anti-FLAG (1:1000) (Sigma-Aldrich, F1804), anti-MRG-1 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% milk in PBS-T and then blotted with a mouse anti-HTT (MAB2166. dilution: 1:5,000; Millipore), rabbit anti β--catenin (Ab2365 ...
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bioRxiv - Immunology 2020Quote: ... Hypochlorous acid (HOCl) was prepared by 1/5 dilution of NaOCl solution with available chlorine 4.00-4.99 % (Sigma Aldrich) in ddH2O containing 0.1M NaOH ...
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bioRxiv - Neuroscience 2019Quote: ... and transferred into ice-cold Hibernate A/B27 medium (50 ml Hibernate A medium with 1 ml B27 and 0.125 ml Glutamax) containing 5 μg/ml actinomycin D (Sigma). Hippocampi were microdissected and incubated for 20 min on ice in Hibernate A/B27 medium containing 1 μM TTX (Tocris) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested after 16 hours and resuspended into 5 mL lysis buffer (200 mM Tris pH 8.0, 500 mM NaCl, 1% (v/v) Triton X-100 (Sigma), 0.02 mg/mL leupeptin ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed in modified Laemmli buffer (5% SDS, 10% glycerol, 32.9 mM Tris-HCl pH 6.8) supplemented with 1 mM DTT (Sigma-Aldrich), and their extracts (15 µG ...
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bioRxiv - Neuroscience 2019Quote: ... source-space power was averaged within standard frequency bands (Delta: 1-4Hz, Theta: 5-8Hz, Alpha: 8.5-12Hz, Sigma: 12.5-17Hz ...
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bioRxiv - Neuroscience 2019Quote: ... R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy] acetic acid (DIOA; Sigma-Aldrich) and VU0240551 (VU ...
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bioRxiv - Genomics 2021Quote: ... One aliquot from each of the two nuclear isolation conditions was then resuspended in 1 mL Nuclear Permeabilization Buffer (1X PBS, 5% Bovine Serum Albumin, 0.2% IGEPAL CA-630 (Sigma), 1 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in TBS-T containing 5% non-fat milk and probed against the primary antibodies: anti-PrP 8H4 (1: 10,000, Millipore), anti-PrP 3F4 (1 ...
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bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Microbiology 2021Quote: ... The culture was then plated on SA (1% glucose) agar plates supplemented with 10 μg/mL 5-Fluoroorotate (Sigma). Plates were incubated at 30 °C until colonies emerge ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich), and subsequently incubated overnight at 4 °C with respective primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells) or for 1 hour with 200 µM EU (HCT116 cells) before fixation with 3.7% formaldehyde (FA, Sigma) in PBS for 15 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024 ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane were then blocked for 1 hr with 5% milk in Tris-buffered saline plus 0.02% Tween20 (Sigma-Aldrich). Membranes were incubated with the primary antibodies O/N at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and then probed with 5 % skim milk (in 1x TBS-T) containing anti-GFP antibody (1:1,000; Sigma-Aldrich) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were minced and digested for 1 hour at 37C in digestion buffer (DMEM, 5% FBS, penicillin, streptomycin, 2.5 mg/ml Collagenase D (Sigma), 0.5 mg/ml Liberase DL (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Neuroscience 2019Quote: ... NaCl, 60; sucrose, 100; KCL, 2.5; NaH2PO4, 1.25; NaHCO3, 26; CaCl2, 1; MgCl2, 5; glucose, 20; from Sigma-Aldrich) and sliced to 300µm ...
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bioRxiv - Genetics 2021Quote: ... in PBS for 20 minutes at 4°C followed by a 1 hour room temperature incubation in blocking solution of 5% donkey serum (Biosera) in 0.3% Triton-X-100 (Sigma) in PBS (0.3% PBST) ...
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bioRxiv - Biochemistry 2021Quote: ... An equal volume of NUN buffer (20mM HEPES pH 7.5, 10mM MgCl2, 300mM NaCl, 0.2mM EDTA, 1M urea, 1% NP-40, 1mM DTT, 1x SigmaFast Protease inhibitor [Sigma]) was gently added to the nuclear suspension and incubated on ice for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and later blocked in 5% milk and probed with the following antibodies: phospo-CREB (1:6000, Millipore #05-667), total-CREB (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspension was transferred to the FACS tube and stained with 5 µl Hoechst (1 mg/ml, Sigma #33342) as a measure for DNA cell content ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with protease inhibitors (2.5 mM sodium fluoride, 1 mM sodium orthovanadate, 0.5 mM phenylmethylsulphonyl fluoride, 100 l of protease inhibitor cocktail [Sigma]). Lysates were rotated for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: Proteins were separated using SDS-PAGE gel composed of bisacrylamide (37:5:1) and blotted onto a polyvinylidene difuoride membrane (0.45-µm pore size; Millipore). Nonspecifc sites were blocked in skimmed milk in the presence of 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2019Quote: ... transferred to a nitrocellulose or PVDF membrane and blocked for 1 hour in 5% bovine serum albumin (Sigma, A7906) in TBS-T ...
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bioRxiv - Pathology 2019Quote: ... 20 mM Tris-HCl [pH 7.4], 1 mM EDTA, 5 mM DTT, and 50 µL protease inhibitor cocktail [Sigma]) and homogenized on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... and used to infect 5×105 MCF10A cells (seeded 1 day before) with polybrene (Sigma-Aldrich, Cat. No. S2667) (8 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma] ...
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bioRxiv - Immunology 2020Quote: ... Fluorophore inactivation with H2O2 was conducted as described previously (5) with tissue sections being treated for 1 hour at room temperature with 4.5% H2O2 (Sigma) prepared in an alkaline solution ...
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bioRxiv - Synthetic Biology 2021Quote: ... The material was then rehydrated by adding 1 × PBS for 5 min followed by incubation with 2% driselase (Sigma) for 60 min at 37°C in a moisture chamber to remove the cell wall ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 ml of fresh media and treated with CCCP (0.1 mM, RT for 5 min, Sigma #C2759) for PMF decoupling ...
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bioRxiv - Microbiology 2020Quote: ... Actin was detected using anti-actin antibody (1:2000 in 5% BSA/TBS-T, Clone AC-74, Sigma-Aldrich). Antibodies were detected using Goat anti-Rabbit IgG-HRP conjugate (1:10000 ...