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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... Cell cycle was examined by flow cytometry following 5′-bromo-2′-deoxyuridine (BrdU) labeling (BrdU: Sigma-Aldrich, MB5002 ...
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bioRxiv - Cancer Biology 2022Quote: ... S-phase populations were identified by incorporation of 10 μM 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 45 min before fixation ...
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bioRxiv - Cell Biology 2022Quote: ... A small piece of frozen BAT (2 – 5 mg) was transferred into a BeadBug tube (Sigma Aldrich) containing 1.0 mm zirconium beads (Merck ...
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bioRxiv - Developmental Biology 2022Quote: Time-pregnant mice were injected with 5-bromo-2’deoxyuridine (BrdU) (50 mg/kg body weight, Sigma) 1 h before the harvest of embryos ...
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bioRxiv - Neuroscience 2023Quote: Mice were injected intraperitoneally (i.p.) once with 150 mg/kg of 5-bromo-2′-deoxyuridine (BrdU; Sigma) 24 hours before sacrifice for cell proliferation assessment ...
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bioRxiv - Neuroscience 2022Quote: ... and ≥99% 4-methyl-3-heptanol and 99% 6-methyl-5-hepten-2-one from Sigma-Aldrich (Item numbers M48309 and M48805-100ML ...
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bioRxiv - Cell Biology 2023Quote: ... The dermis was injected with approximately 2-5 μl TAT-Cre recombinase (200 U/ml; Sigma-Aldrich) using a glass microcapillary pipette ...
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bioRxiv - Genomics 2023Quote: ... about 107 exponentially growing cells were pulse-labelled with 5-Bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich #B9285) for 1 h and sorted into four S-phase fractions ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA was stained with 5 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, D9542-5MG, SIGMA) diluted in 2% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... and (+−)-6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. Sodium hydroxide (cat ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins were loaded in the Laemmli sample buffer supplemented with 5% 2-mercaptoethanol (Sigma, catalog #: M3148) to reduce the disulfide bonds ...
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bioRxiv - Pathology 2024Quote: ... and treated with or without 5 ng/mL TGFβ1 and GSK2193874 (2 and 10 μM, Sigma Aldrich) for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Day 4 and Day 8 were treated with 10 μM 5-bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich) for 1 hour at 37 °C in a CO2 incubator ...
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bioRxiv - Immunology 2024Quote: ... paraffin sections were first blocked with 5% goat serum and 2% fish gelatin (both from Sigma-Aldrich) at 25°C for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were immediately placed in a solution of 50 µm 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) and were allowed to develop in this solution.
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bioRxiv - Developmental Biology 2024Quote: ... cells were labeled with 20 mM 5-chloro-2-deoxyuridine (CldU, Sigma-Aldrich by Merck, Darmstadt, Germany) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg of protein was reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma Aldrich) for 2 min and alkylated with 20 mM chloroacetamide (CAA ...
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bioRxiv - Neuroscience 2024Quote: ... 8-cyclopentyl-1,3-dimethylxanthine (CPT; 3 nmol in 5 μl saline of 2% DMSO; #C102; Sigma-Aldrich; injection was given immediately before the start of exposure to restraint stress or 30 min before intrathecal injection of NA).
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bioRxiv - Neuroscience 2024Quote: ... the NMDA receptor blocker DL-2-Amino-5-phosphonopentanoic acid at 50 µM (APV; A5282, Sigma-Aldrich), the AMPA receptor blocker 2,3-dioxo-6-nitro-7-sulfamoyl-benzo[f]quinoxaline at 10 µM] (NBQX ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by a second 20-minute pulse with 250 µM 5-chloro-2-deoxyuridine (C6891, Sigma-Aldrich). The labeled cells were then washed twice with ice-cold 1X PBS ...
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bioRxiv - Physiology 2024Quote: 5-Bromo-2’-Deoxyuridine (BrdU) incorporation assay was performed in accordance with the manufacturer’s instruction (Millipore, #2752) 18 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mL of linear gradient of 0 mM to 2 mM of mannose 6-phosphate (Sigma, M3655) by mixing buffer A and buffer B (50 mM NaOAc ...
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bioRxiv - Biophysics 2024Quote: ... Cells were suspended in PBS +2%FBS with 5 mg/mL FITC-conjugated dextran (Sigma, FD2000S-250MG). When no cell was present at the detection region ...
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bioRxiv - Cancer Biology 2024Quote: Zebrafish larvae were dechorionated at 2 dpf using fine forceps (Jewelers forceps Dumont No.5, Sigma-Aldrich) under stereo microscope and anesthetized in 0.002% MS-222 (tricaine methanesulfonate ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µl of a 12% (w/v) solution of poly(2-hydroxyethyl methacrylate) (poly-HEMA; Sigma Aldrich) dissolved in ethanol and 1 µl of a 10% (w/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by five minutes in 2% peracetic acid (Supelco, Denmark: Cas number: 79-21-0) and a rinse in 5 mL sterile water (Sigma-Aldrich, Germany, Cas number: 7732-18-5) as per the protocol by Tegtmeier et al ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were counterstained with 5 μM 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, Cat# D9542) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... P3 and P6 pups were injected intraperitoneally with 50 mg/kg of EdU (5-ethyl-2-deoxyuridine; Sigma). Brains were harvested 2h after injection and processed for frozen tissue sections ...
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bioRxiv - Biochemistry 2021Quote: ... with 10% ADC supplement (5% BSA, 0.85% NaCl, 2% dextrose) and 0.05% tyloxapol or 7H10-ADC agar medium (Sigma) at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Neuroscience 2020Quote: ... (+)-Bicuculline (No. 14340) and DL-2-amino-5-phosphonopentanoinc acid (APV; No. A5282) were obtained from Sigma-Aldrich; 6,7-dinitroquinoxaline-2,3(1H,4H)-dione disodium salt (DNQX ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue was then incubated in CUBIC-1A solution (10% Triton, 5% NNNN-tetrakis (2-HP) ethylenediamine (Sigma, 122262), 10% Urea ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were stimulated with 50 U/ml IL-2 and 5◻μg/ml phytohemagglutinin (PHA; Sigma-Aldrich) for 72 h prior to infection or transduction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with 0.1 mL serially diluted supernatant containing SARS-CoV-2 for 30 minutes at 37°C and 5% CO2 before being left in 1.5mL overlay (DMEM, 2% FBS, 1.2% cellulose microcrystalline (Sigma)) at 37°C and 5% CO2 for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... After a 2 h blocking step in blocking buffer (PBX with 5% normal goat serum, Sigma-Aldrich, G9023) at RT ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2) for 2 min and then the DNA digested with 20U DNaseI (cat #: D4527, Sigma-Aldrich) in 1 mL of digestion buffer for 40 sec at RT ...
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bioRxiv - Genetics 2022Quote: ... Experiments for Figure 5 were as Figure 2 with the following changes: 10 μg/mL tunicamycin (InSolution, Sigma) was used ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 5% SDS supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich). The following antibodies were purchased from Cell Signaling Technology (Danvers) ...
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bioRxiv - Plant Biology 2021Quote: ... The full length coding sequence of AdACS1/2/3 and AdACO3/5 were inserted into pET-32a (Novagen) vector and then transferred into Escherichia coli strain BL21 (DE3) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody in 250 μl of cold antibody buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 2 mM EDTA, 0.1% digitonin, and protease inhibitor cocktails from Sigma) was added to the tubes and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spinesbioRxiv - Neuroscience 2021Quote: ... and in addition of 5′fluoro-2-deoxyuridine + uridine (FUDR) (Sigma; 20 μg and 50 μg/ml, respectively), to block glial proliferation ...
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bioRxiv - Physiology 2022Quote: ... L4 staged worms were picked onto NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma F0503), 50 μg/ml carbenicillin ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 5 min with 10 μg/ml 4’,6-Diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to stain cell nuclei ...
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bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...